More than 300 homologs were found in PanDaTox collection
for query gene Vapar_3595 on replicon NC_012791
Organism: Variovorax paradoxus S110



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Replicon accession
Locus tag
Product
% identity
Alignment on query gene
Alignment on homolog gene
Length
Bit score

E-value
Organism name
Kingdom
Plasmid clonability
Plasmid unclonability p-value
Fosmid clonability
Fosmid unclonability p-value
Coverage Plot
PanDaTox homologs
Experimental validation
NC_012791  Vapar_3595  two component transcriptional regulator, LuxR family  100 
 
 		 225 aa  451  1.0000000000000001e-126  Variovorax paradoxus S110  Bacteria  normal  0.414086  n/a   
 
 
-
 
NC_012791  Vapar_3592  two component transcriptional regulator, LuxR family  57.02 
 
 		 235 aa  239  2e-62  Variovorax paradoxus S110  Bacteria  normal  0.356082  n/a   
 
 
-
 
NC_007947  Mfla_0447  two component LuxR family transcriptional regulator  41.43 
 
 		 244 aa  154  1e-36  Methylobacillus flagellatus KT  Bacteria  normal  0.013193  normal  0.6128 
 
 
-
 
NC_007973  Rmet_0612  two component LuxR family transcriptional regulator  38.43 
 
 		 231 aa  148  8e-35  Cupriavidus metallidurans CH34  Bacteria  normal  1  normal  0.105579 
 
 
-
 
NC_007974  Rmet_5165  two component LuxR family transcriptional regulator  40.57 
 
 		 231 aa  134  9.999999999999999e-31  Cupriavidus metallidurans CH34  Bacteria  normal  1  normal  0.162111 
 
 
-
 
NC_007347  Reut_A1734  two component LuxR family transcriptional regulator  38.57 
 
 		 257 aa  116  1.9999999999999998e-25  Ralstonia eutropha JMP134  Bacteria  normal  0.159279  n/a   
 
 
-
 
NC_012791  Vapar_4439  two component transcriptional regulator, LuxR family  36.49 
 
 		 224 aa  109  4.0000000000000004e-23  Variovorax paradoxus S110  Bacteria  normal  1  n/a   
 
 
-
 
NC_010524  Lcho_3952  two component LuxR family transcriptional regulator  34.1 
 
 		 222 aa  97.8  1e-19  Leptothrix cholodnii SP-6  Bacteria  n/a    normal  0.173353 
 
 
-
 
NC_013525  Tter_1109  two component transcriptional regulator, LuxR family  30.33 
 
 		 218 aa  95.9  4e-19  Thermobaculum terrenum ATCC BAA-798  Bacteria  n/a    n/a   
 
 
-
 
NC_008825  Mpe_A3237  response regulator  35 
 
 		 219 aa  94  2e-18  Methylibium petroleiphilum PM1  Bacteria  normal  1  normal  1 
 
 
-
 
NC_014248  Aazo_0236  LuxR family two component transcriptional regulator  28.23 
 
 		 218 aa  93.2  3e-18  'Nostoc azollae' 0708  Bacteria  normal  1  n/a   
 
 
-
 
NC_013510  Tcur_1532  two component transcriptional regulator, LuxR family  30.65 
 
 		 220 aa  92.4  5e-18  Thermomonospora curvata DSM 43183  Bacteria  normal  0.0114765  n/a   
 
 
-
 
NC_013595  Sros_0553  response regulator receiver protein  30.5 
 
 		 213 aa  92  6e-18  Streptosporangium roseum DSM 43021  Bacteria  normal  0.82888  normal  1 
 
 
-
 
NC_009012  Cthe_1267  two component LuxR family transcriptional regulator  27.36 
 
 		 216 aa  90.9  1e-17  Clostridium thermocellum ATCC 27405  Bacteria  normal  1  n/a   
 
 
-
 
NC_008148  Rxyl_1550  two component LuxR family transcriptional regulator  32.51 
 
 		 220 aa  89  5e-17  Rubrobacter xylanophilus DSM 9941  Bacteria  normal  1  n/a   
 
 
-
 
NC_013947  Snas_5788  two component transcriptional regulator, LuxR family  33.33 
 
 		 214 aa  89  5e-17  Stackebrandtia nassauensis DSM 44728  Bacteria  normal  1  normal  0.785726 
 
 
-
 
NC_007413  Ava_2028  two component LuxR family transcriptional regulator  26.67 
 
 		 219 aa  87.4  1e-16  Anabaena variabilis ATCC 29413  Bacteria  normal  0.515359  normal  1 
 
 
-
 
NC_013595  Sros_1417  response regulator receiver protein  29.56 
 
 		 234 aa  87.8  1e-16  Streptosporangium roseum DSM 43021  Bacteria  normal  1  normal  0.0224272 
 
 
-
 
NC_007974  Rmet_4986  two component LuxR family transcriptional regulator  30.81 
 
 		 237 aa  87.8  1e-16  Cupriavidus metallidurans CH34  Bacteria  normal  0.170484  normal  0.313407 
 
 
-
 
NC_013124  Afer_1446  two component transcriptional regulator, LuxR family  33.76 
 
 		 223 aa  88.2  1e-16  Acidimicrobium ferrooxidans DSM 10331  Bacteria  normal  1  n/a   
 
 
-
 
NC_011729  PCC7424_0807  two component transcriptional regulator, LuxR family  25.24 
 
 		 228 aa  86.7  3e-16  Cyanothece sp. PCC 7424  Bacteria  n/a    normal  0.218712 
 
 
-
 
NC_011831  Cagg_0340  two component transcriptional regulator, LuxR family  28.71 
 
 		 217 aa  85.9  4e-16  Chloroflexus aggregans DSM 9485  Bacteria  hitchhiker  0.00614189  hitchhiker  0.00317709 
 
 
-
 
NC_007404  Tbd_2464  two component LuxR family transcriptional regulator  28.84 
 
 		 221 aa  85.9  5e-16  Thiobacillus denitrificans ATCC 25259  Bacteria  normal  1  normal  1 
 
 
-
 
NC_013521  Sked_03540  response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain  28.63 
 
 		 226 aa  84.7  0.000000000000001  Sanguibacter keddieii DSM 10542  Bacteria  normal  1  normal  1 
 
 
-
 
NC_008148  Rxyl_2809  two component LuxR family transcriptional regulator  31.03 
 
 		 219 aa  83.6  0.000000000000002  Rubrobacter xylanophilus DSM 9941  Bacteria  normal  1  n/a   
 
 
-
 
NC_008789  Hhal_1965  two component LuxR family transcriptional regulator  31.88 
 
 		 217 aa  84  0.000000000000002  Halorhodospira halophila SL1  Bacteria  normal  1  n/a   
 
 
-
 
NC_013595  Sros_3331  response regulator receiver protein  29.15 
 
 		 227 aa  83.6  0.000000000000002  Streptosporangium roseum DSM 43021  Bacteria  normal  0.832029  normal  0.250763 
 
 
-
 
NC_013525  Tter_0300  two component transcriptional regulator, LuxR family  25.63 
 
 		 224 aa  82.8  0.000000000000003  Thermobaculum terrenum ATCC BAA-798  Bacteria  n/a    n/a   
 
 
-
 
NC_014165  Tbis_0775  LuxR family two component transcriptional regulator  29.56 
 
 		 234 aa  83.2  0.000000000000003  Thermobispora bispora DSM 43833  Bacteria  normal  1  normal  0.336237 
 
 
-
 
NC_008025  Dgeo_0555  two component LuxR family transcriptional regulator  31.65 
 
 		 228 aa  82.8  0.000000000000004  Deinococcus geothermalis DSM 11300  Bacteria  normal  1  normal  1 
 
 
-
 
NC_008578  Acel_0518  two component LuxR family transcriptional regulator  32.72 
 
 		 225 aa  82.8  0.000000000000004  Acidothermus cellulolyticus 11B  Bacteria  normal  1  normal  0.665179 
 
 
-
 
NC_011901  Tgr7_2171  two component transcriptional regulator, LuxR family  30.39 
 
 		 227 aa  82  0.000000000000006  Thioalkalivibrio sp. HL-EbGR7  Bacteria  normal  1  n/a   
 
 
-
 
NC_013947  Snas_3040  two component transcriptional regulator, LuxR family  30.45 
 
 		 224 aa  82.4  0.000000000000006  Stackebrandtia nassauensis DSM 44728  Bacteria  normal  0.0154763  normal  1 
 
 
-
 
NC_013132  Cpin_3102  two component transcriptional regulator, LuxR family  27.57 
 
 		 222 aa  82  0.000000000000006  Chitinophaga pinensis DSM 2588  Bacteria  normal  0.774177  normal  0.15342 
 
 
-
 
NC_009767  Rcas_3262  two component LuxR family transcriptional regulator  32.47 
 
 		 222 aa  82  0.000000000000006  Roseiflexus castenholzii DSM 13941  Bacteria  normal  1  normal  0.764026 
 
 
-
 
NC_007333  Tfu_2950  LuxR response regulator receiver  29.31 
 
 		 221 aa  82  0.000000000000007  Thermobifida fusca YX  Bacteria  normal  1  n/a   
 
 
-
 
NC_009523  RoseRS_1633  two component LuxR family transcriptional regulator  33.94 
 
 		 254 aa  82  0.000000000000007  Roseiflexus sp. RS-1  Bacteria  normal  1  normal  1 
 
 
-
 
NC_007333  Tfu_2491  LuxR response regulator receiver  31.43 
 
 		 242 aa  81.6  0.000000000000009  Thermobifida fusca YX  Bacteria  normal  0.470277  n/a   
 
 
-
 
NC_007519  Dde_0977  two component LuxR family transcriptional regulator  26.96 
 
 		 223 aa  81.6  0.000000000000009  Desulfovibrio desulfuricans subsp. desulfuricans str. G20  Bacteria  decreased coverage  0.00800748  n/a   
 
 
-
 
NC_011831  Cagg_1769  two component transcriptional regulator, LuxR family  30 
 
 		 219 aa  81.6  0.000000000000009  Chloroflexus aggregans DSM 9485  Bacteria  normal  0.221401  normal  0.0578747 
 
 
-
 
NC_013595  Sros_1446  response regulator receiver protein  29.82 
 
 		 209 aa  81.3  0.00000000000001  Streptosporangium roseum DSM 43021  Bacteria  normal  0.0167897  normal  0.0778626 
 
 
-
 
NC_007948  Bpro_2543  two component LuxR family transcriptional regulator  28.17 
 
 		 230 aa  81.3  0.00000000000001  Polaromonas sp. JS666  Bacteria  normal  0.149636  normal  0.0443917 
 
 
-
 
NC_009767  Rcas_1142  two component LuxR family transcriptional regulator  29.13 
 
 		 222 aa  81.3  0.00000000000001  Roseiflexus castenholzii DSM 13941  Bacteria  normal  0.661777  normal  1 
 
 
-
 
NC_009523  RoseRS_3421  two component LuxR family transcriptional regulator  30.1 
 
 		 223 aa  81.3  0.00000000000001  Roseiflexus sp. RS-1  Bacteria  normal  1  normal  0.0556432 
 
 
-
 
NC_009523  RoseRS_1984  two component LuxR family transcriptional regulator  31.82 
 
 		 220 aa  80.9  0.00000000000001  Roseiflexus sp. RS-1  Bacteria  normal  0.178457  normal  0.123783 
 
 
-
 
NC_013124  Afer_0180  two component transcriptional regulator, LuxR family  39.39 
 
 		 216 aa  81.3  0.00000000000001  Acidimicrobium ferrooxidans DSM 10331  Bacteria  normal  0.245465  n/a   
 
 
-
 
NC_003909  BCE_1102  LuxR family DNA-binding response regulator  26.37 
 
 		 214 aa  80.9  0.00000000000002  Bacillus cereus ATCC 10987  Bacteria  normal  1  n/a   
 
 
-
 
NC_007519  Dde_2674  two component LuxR family transcriptional regulator  27.36 
 
 		 215 aa  80.9  0.00000000000002  Desulfovibrio desulfuricans subsp. desulfuricans str. G20  Bacteria  normal  0.721789  n/a   
 
 
-
 
NC_009972  Haur_3654  two component LuxR family transcriptional regulator  30.97 
 
 		 221 aa  80.1  0.00000000000002  Herpetosiphon aurantiacus ATCC 23779  Bacteria  hitchhiker  0.0000210563  n/a   
 
 
-
 
NC_007777  Francci3_1039  two component LuxR family transcriptional regulator  28.64 
 
 		 238 aa  80.1  0.00000000000002  Frankia sp. CcI3  Bacteria  normal  1  normal  0.135118 
 
 
-
 
NC_013526  Tter_2548  two component transcriptional regulator, LuxR family  27.7 
 
 		 218 aa  80.1  0.00000000000002  Thermobaculum terrenum ATCC BAA-798  Bacteria  normal  1  n/a   
 
 
-
 
NC_010571  Oter_0829  two component LuxR family transcriptional regulator  28.66 
 
 		 219 aa  80.9  0.00000000000002  Opitutus terrae PB90-1  Bacteria  normal  1  normal  0.0121621 
 
 
-
 
NC_008781  Pnap_3628  two component LuxR family transcriptional regulator  29.55 
 
 		 292 aa  80.5  0.00000000000002  Polaromonas naphthalenivorans CJ2  Bacteria  normal  1  normal  0.350857 
 
 
-
 
NC_010002  Daci_3380  two component LuxR family transcriptional regulator  27.62 
 
 		 259 aa  80.1  0.00000000000003  Delftia acidovorans SPH-1  Bacteria  normal  1  normal  0.277634 
 
 
-
 
NC_009972  Haur_1563  two component LuxR family transcriptional regulator  29.38 
 
 		 257 aa  80.1  0.00000000000003  Herpetosiphon aurantiacus ATCC 23779  Bacteria  normal  0.781509  n/a   
 
 
-
 
NC_009921  Franean1_5840  two component LuxR family transcriptional regulator  31.61 
 
 		 237 aa  79.7  0.00000000000003  Frankia sp. EAN1pec  Bacteria  normal  0.0206065  normal  0.380036 
 
 
-
 
NC_010184  BcerKBAB4_0919  two component LuxR family transcriptional regulator  26.7 
 
 		 214 aa  79.3  0.00000000000004  Bacillus weihenstephanensis KBAB4  Bacteria  normal  1  n/a   
 
 
-
 
NC_013501  Rmar_1005  two component transcriptional regulator, LuxR family  33.13 
 
 		 211 aa  79.3  0.00000000000004  Rhodothermus marinus DSM 4252  Bacteria  normal  1  n/a   
 
 
-
 
NC_014210  Ndas_4749  two component transcriptional regulator, LuxR family  29.22 
 
 		 223 aa  79.3  0.00000000000004  Nocardiopsis dassonvillei subsp. dassonvillei DSM 43111  Bacteria  normal  1  normal  1 
 
 
-
 
NC_014148  Plim_0373  response regulator receiver  29.89 
 
 		 220 aa  79.3  0.00000000000005  Planctomyces limnophilus DSM 3776  Bacteria  normal  1  n/a   
 
 
-
 
NC_013595  Sros_5304  response regulator receiver protein  29.58 
 
 		 220 aa  79  0.00000000000006  Streptosporangium roseum DSM 43021  Bacteria  normal  0.128918  normal  1 
 
 
-
 
NC_009921  Franean1_5512  two component LuxR family transcriptional regulator  29.7 
 
 		 250 aa  78.6  0.00000000000007  Frankia sp. EAN1pec  Bacteria  normal  1  normal  0.631776 
 
 
-
 
NC_013093  Amir_5375  two component transcriptional regulator, LuxR family  30.1 
 
 		 218 aa  78.6  0.00000000000008  Actinosynnema mirum DSM 43827  Bacteria  normal  1  n/a   
 
 
-
 
NC_011992  Dtpsy_1772  two component transcriptional regulator, LuxR family  28.3 
 
 		 262 aa  78.2  0.00000000000009  Acidovorax ebreus TPSY  Bacteria  normal  1  n/a   
 
 
-
 
NC_008146  Mmcs_1056  two component LuxR family transcriptional regulator  30.85 
 
 		 219 aa  78.2  0.00000000000009  Mycobacterium sp. MCS  Bacteria  normal  1  n/a   
 
 
-
 
NC_008705  Mkms_1072  two component LuxR family transcriptional regulator  30.85 
 
 		 219 aa  78.2  0.00000000000009  Mycobacterium sp. KMS  Bacteria  normal  0.562029  normal  0.0679961 
 
 
-
 
NC_009077  Mjls_1083  two component LuxR family transcriptional regulator  30.85 
 
 		 219 aa  78.2  0.00000000000009  Mycobacterium sp. JLS  Bacteria  normal  0.605869  normal  1 
 
 
-
 
NC_013530  Xcel_1619  two component transcriptional regulator, LuxR family  31.76 
 
 		 234 aa  77.8  0.0000000000001  Xylanimonas cellulosilytica DSM 15894  Bacteria  normal  1  n/a   
 
 
-
 
NC_009767  Rcas_1355  two component LuxR family transcriptional regulator  26.27 
 
 		 230 aa  77.8  0.0000000000001  Roseiflexus castenholzii DSM 13941  Bacteria  normal  1  normal  1 
 
 
-
 
NC_013595  Sros_7606  response regulator receiver protein  33.94 
 
 		 214 aa  77.8  0.0000000000001  Streptosporangium roseum DSM 43021  Bacteria  normal  1  normal  1 
 
 
-
 
NC_010625  Bphy_5911  two component LuxR family transcriptional regulator  30.26 
 
 		 219 aa  77.8  0.0000000000001  Burkholderia phymatum STM815  Bacteria  normal  1  normal  1 
 
 
-
 
NC_008781  Pnap_1851  two component LuxR family transcriptional regulator  28.57 
 
 		 262 aa  77.8  0.0000000000001  Polaromonas naphthalenivorans CJ2  Bacteria  normal  0.467917  normal  0.53175 
 
 
-
 
NC_013159  Svir_38420  response regulator containing a CheY-like receiver domain protein and an HTH DNA-binding domain protein  31.19 
 
 		 226 aa  77.8  0.0000000000001  Saccharomonospora viridis DSM 43017  Bacteria  normal  1  normal  1 
 
 
-
 
NC_013131  Caci_5202  two component transcriptional regulator, LuxR family  31.13 
 
 		 225 aa  78.2  0.0000000000001  Catenulispora acidiphila DSM 44928  Bacteria  normal  0.677169  normal  0.920773 
 
 
-
 
NC_009455  DehaBAV1_0632  two component LuxR family transcriptional regulator  27.43 
 
 		 224 aa  77.8  0.0000000000001  Dehalococcoides sp. BAV1  Bacteria  hitchhiker  0.00385785  n/a   
 
 
-
 
NC_009921  Franean1_3185  two component LuxR family transcriptional regulator  29.76 
 
 		 223 aa  77  0.0000000000002  Frankia sp. EAN1pec  Bacteria  normal  0.704721  normal  1 
 
 
-
 
NC_011830  Dhaf_2789  two component transcriptional regulator, LuxR family  27.06 
 
 		 212 aa  77.4  0.0000000000002  Desulfitobacterium hafniense DCB-2  Bacteria  normal  0.246733  n/a   
 
 
-
 
NC_009767  Rcas_2258  two component LuxR family transcriptional regulator  34.36 
 
 		 254 aa  77.4  0.0000000000002  Roseiflexus castenholzii DSM 13941  Bacteria  normal  1  hitchhiker  0.00245701 
 
 
-
 
NC_007333  Tfu_2582  LuxR response regulator receiver  28.4 
 
 		 233 aa  77  0.0000000000002  Thermobifida fusca YX  Bacteria  normal  1  n/a   
 
 
-
 
NC_013947  Snas_2579  two component transcriptional regulator, LuxR family  27.8 
 
 		 213 aa  77.4  0.0000000000002  Stackebrandtia nassauensis DSM 44728  Bacteria  normal  1  normal  0.0500394 
 
 
-
 
NC_014158  Tpau_4145  two component transcriptional regulator, LuxR family  30.52 
 
 		 216 aa  77.4  0.0000000000002  Tsukamurella paurometabola DSM 20162  Bacteria  normal  1  n/a   
 
 
-
 
NC_008697  Noca_4902  response regulator receiver  28.71 
 
 		 216 aa  77.4  0.0000000000002  Nocardioides sp. JS614  Bacteria  normal  0.443208  normal  0.676579 
 
 
-
 
NC_009338  Mflv_5014  two component LuxR family transcriptional regulator  32.67 
 
 		 209 aa  77  0.0000000000002  Mycobacterium gilvum PYR-GCK  Bacteria  normal  0.103708  normal  0.29037 
 
 
-
 
NC_013947  Snas_5595  two component transcriptional regulator, LuxR family  29.47 
 
 		 215 aa  77.4  0.0000000000002  Stackebrandtia nassauensis DSM 44728  Bacteria  normal  1  normal  1 
 
 
-
 
NC_014212  Mesil_2510  two component transcriptional regulator, LuxR family  30 
 
 		 217 aa  76.6  0.0000000000002  Meiothermus silvanus DSM 9946  Bacteria  normal  1  normal  1 
 
 
-
 
NC_013061  Phep_1616  response regulator receiver  23.81 
 
 		 210 aa  76.3  0.0000000000003  Pedobacter heparinus DSM 2366  Bacteria  normal  0.869432  normal  1 
 
 
-
 
NC_013947  Snas_6085  two component transcriptional regulator, LuxR family  31.71 
 
 		 217 aa  76.6  0.0000000000003  Stackebrandtia nassauensis DSM 44728  Bacteria  normal  1  normal  1 
 
 
-
 
NC_009632  SaurJH1_1936  response regulator receiver  22.55 
 
 		 207 aa  76.6  0.0000000000003  Staphylococcus aureus subsp. aureus JH1  Bacteria  unclonable  0.000000150819  n/a   
 
 
-
 
NC_013170  Ccur_06630  response regulator containing a CheY-like receiver domain protein and an HTH DNA-binding domain protein  25.49 
 
 		 215 aa  76.3  0.0000000000003  Cryptobacterium curtum DSM 15641  Bacteria  normal  0.252447  normal  0.303532 
 
 
-
 
NC_009972  Haur_1600  two component LuxR family transcriptional regulator  31.16 
 
 		 207 aa  76.6  0.0000000000003  Herpetosiphon aurantiacus ATCC 23779  Bacteria  normal  1  n/a   
 
 
-
 
NC_007777  Francci3_3253  two component LuxR family transcriptional regulator  29.49 
 
 		 213 aa  76.6  0.0000000000003  Frankia sp. CcI3  Bacteria  normal  1  normal  1 
 
 
-
 
NC_013530  Xcel_0190  two component transcriptional regulator, LuxR family  31.82 
 
 		 219 aa  76.3  0.0000000000003  Xylanimonas cellulosilytica DSM 15894  Bacteria  normal  0.146862  n/a   
 
 
-
 
NC_013131  Caci_2656  two component transcriptional regulator, LuxR family  31.34 
 
 		 226 aa  76.6  0.0000000000003  Catenulispora acidiphila DSM 44928  Bacteria  normal  0.161573  normal  1 
 
 
-
 
NC_009487  SaurJH9_1902  two component LuxR family transcriptional regulator  22.55 
 
 		 207 aa  76.6  0.0000000000003  Staphylococcus aureus subsp. aureus JH9  Bacteria  hitchhiker  0.00000041006  n/a   
 
 
-
 
NC_013385  Adeg_1034  two component transcriptional regulator, LuxR family  26.76 
 
 		 213 aa  75.9  0.0000000000004  Ammonifex degensii KC4  Bacteria  normal  1  n/a   
 
 
-
 
NC_007777  Francci3_0765  two component LuxR family transcriptional regulator  30.23 
 
 		 237 aa  76.3  0.0000000000004  Frankia sp. CcI3  Bacteria  normal  0.272345  normal  1 
 
 
-
 
NC_013235  Namu_4738  two component transcriptional regulator, LuxR family  29.45 
 
 		 233 aa  76.3  0.0000000000004  Nakamurella multipartita DSM 44233  Bacteria  normal  1  normal  1 
 
 
-
 
NC_011899  Hore_19700  two component transcriptional regulator, LuxR family  24.85 
 
 		 209 aa  76.3  0.0000000000004  Halothermothrix orenii H 168  Bacteria  normal  1  n/a   
 
 
-
 
NC_008726  Mvan_1358  two component LuxR family transcriptional regulator  29.95 
 
 		 220 aa  76.3  0.0000000000004  Mycobacterium vanbaalenii PYR-1  Bacteria  normal  0.110964  normal  1 
 
 
-
 
NC_011725  BCB4264_A5535  DNA-binding response regulator  26.11 
 
 		 215 aa  76.3  0.0000000000004  Bacillus cereus B4264  Bacteria  normal  1  n/a   
 
 
-
 
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