More than 300 homologs were found in PanDaTox collection
for query gene RPB_3522 on replicon NC_007778
Organism: Rhodopseudomonas palustris HaA2



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Replicon accession

Locus tag

Product

% identity

Alignment on query gene

Alignment on homolog gene

Length

Bit score

E-value

Organism name

Kingdom

Plasmid clonability

Plasmid unclonability p-value

Fosmid clonability

Fosmid unclonability p-value

Coverage Plot

PanDaTox homologs

Experimental validation
NC_007778  RPB_3522  methyl-accepting chemotaxis sensory transducer  100 
 
 
471 aa  937    Rhodopseudomonas palustris HaA2  Bacteria  normal  0.392623  normal  0.542302 
 
 
-
 
NC_009485  BBta_5250  hypothetical protein  43.04 
 
 
466 aa  323  3e-87  Bradyrhizobium sp. BTAi1  Bacteria  normal  0.869882  normal  0.134387 
 
 
-
 
NC_008789  Hhal_2165  methyl-accepting chemotaxis sensory transducer  36.64 
 
 
470 aa  252  1e-65  Halorhodospira halophila SL1  Bacteria  normal  0.623772  n/a   
 
 
-
 
NC_010814  Glov_3525  methyl-accepting chemotaxis sensory transducer  34.1 
 
 
658 aa  181  2.9999999999999997e-44  Geobacter lovleyi SZ  Bacteria  normal  n/a   
 
 
-
 
NC_002939  GSU1032  methyl-accepting chemotaxis protein  31.86 
 
 
645 aa  175  9.999999999999999e-43  Geobacter sulfurreducens PCA  Bacteria  normal  n/a   
 
 
-
 
NC_011830  Dhaf_4430  methyl-accepting chemotaxis sensory transducer  31.13 
 
 
641 aa  163  7e-39  Desulfitobacterium hafniense DCB-2  Bacteria  normal  n/a   
 
 
-
 
NC_009483  Gura_0375  methyl-accepting chemotaxis sensory transducer  31.38 
 
 
674 aa  162  1e-38  Geobacter uraniireducens Rf4  Bacteria  normal  0.129925  n/a   
 
 
-
 
NC_012918  GM21_0365  methyl-accepting chemotaxis sensory transducer  30.07 
 
 
700 aa  160  4e-38  Geobacter sp. M21  Bacteria  n/a    normal  0.0784998 
 
 
-
 
NC_009483  Gura_3394  methyl-accepting chemotaxis sensory transducer  33.24 
 
 
664 aa  160  6e-38  Geobacter uraniireducens Rf4  Bacteria  decreased coverage  0.00000171268  n/a   
 
 
-
 
NC_011146  Gbem_0383  methyl-accepting chemotaxis sensory transducer  32.04 
 
 
700 aa  159  1e-37  Geobacter bemidjiensis Bem  Bacteria  normal  0.424843  n/a   
 
 
-
 
NC_007520  Tcr_1897  methyl-accepting chemotaxis sensory transducer with Pas/Pac sensor  31.59 
 
 
1170 aa  146  6e-34  Thiomicrospira crunogena XCL-2  Bacteria  normal  n/a   
 
 
-
 
NC_010725  Mpop_2766  methyl-accepting chemotaxis sensory transducer  33.69 
 
 
457 aa  139  1e-31  Methylobacterium populi BJ001  Bacteria  normal  normal  0.626967 
 
 
-
 
NC_010505  Mrad2831_4648  methyl-accepting chemotaxis sensory transducer  36.89 
 
 
414 aa  133  5e-30  Methylobacterium radiotolerans JCM 2831  Bacteria  normal  normal  0.087359 
 
 
-
 
NC_011757  Mchl_2869  methyl-accepting chemotaxis sensory transducer  35.05 
 
 
457 aa  133  6.999999999999999e-30  Methylobacterium chloromethanicum CM4  Bacteria  normal  0.394576  normal 
 
 
-
 
NC_010172  Mext_2643  chemotaxis sensory transducer  35.05 
 
 
457 aa  133  6.999999999999999e-30  Methylobacterium extorquens PA1  Bacteria  normal  normal  0.861208 
 
 
-
 
NC_010001  Cphy_0614  methyl-accepting chemotaxis sensory transducer  29.77 
 
 
429 aa  132  1.0000000000000001e-29  Clostridium phytofermentans ISDg  Bacteria  normal  0.0371385  n/a   
 
 
-
 
NC_010524  Lcho_2167  methyl-accepting chemotaxis sensory transducer  32.77 
 
 
678 aa  132  1.0000000000000001e-29  Leptothrix cholodnii SP-6  Bacteria  n/a    normal  0.195593 
 
 
-
 
NC_008789  Hhal_1714  methyl-accepting chemotaxis sensory transducer  35.76 
 
 
673 aa  129  8.000000000000001e-29  Halorhodospira halophila SL1  Bacteria  normal  0.712235  n/a   
 
 
-
 
NC_007517  Gmet_2939  methyl-accepting chemotaxis sensory transducer  34.81 
 
 
696 aa  129  1.0000000000000001e-28  Geobacter metallireducens GS-15  Bacteria  normal  hitchhiker  0.00000015868 
 
 
-
 
NC_009831  Ssed_3705  methyl-accepting chemotaxis sensory transducer  35.1 
 
 
541 aa  127  5e-28  Shewanella sediminis HAW-EB3  Bacteria  normal  0.0963791  normal 
 
 
-
 
NC_013456  VEA_004525  methyl-accepting chemotaxis protein  35.5 
 
 
418 aa  125  1e-27  Vibrio sp. Ex25  Bacteria  normal  n/a   
 
 
-
 
NC_008576  Mmc1_0310  methyl-accepting chemotaxis sensory transducer  25.88 
 
 
994 aa  125  2e-27  Magnetococcus sp. MC-1  Bacteria  normal  normal 
 
 
-
 
NC_009783  VIBHAR_00866  hypothetical protein  35.11 
 
 
418 aa  124  5e-27  Vibrio harveyi ATCC BAA-1116  Bacteria  n/a    n/a   
 
 
-
 
NC_009456  VC0395_0185  methyl-accepting chemotaxis protein  34.2 
 
 
770 aa  122  9.999999999999999e-27  Vibrio cholerae O395  Bacteria  normal  n/a   
 
 
-
 
NC_013174  Jden_0359  methyl-accepting chemotaxis sensory transducer  32.06 
 
 
542 aa  122  9.999999999999999e-27  Jonesia denitrificans DSM 20603  Bacteria  normal  normal 
 
 
-
 
NC_010501  PputW619_1254  methyl-accepting chemotaxis sensory transducer  36.92 
 
 
639 aa  122  1.9999999999999998e-26  Pseudomonas putida W619  Bacteria  normal  0.523103  normal  0.0331864 
 
 
-
 
NC_010814  Glov_0882  methyl-accepting chemotaxis sensory transducer  33.55 
 
 
540 aa  121  1.9999999999999998e-26  Geobacter lovleyi SZ  Bacteria  normal  0.566239  n/a   
 
 
-
 
NC_011126  HY04AAS1_1329  methyl-accepting chemotaxis sensory transducer  31.19 
 
 
473 aa  121  3e-26  Hydrogenobaculum sp. Y04AAS1  Bacteria  normal  n/a   
 
 
-
 
NC_007954  Sden_0712  chemotaxis sensory transducer  34.77 
 
 
541 aa  120  4.9999999999999996e-26  Shewanella denitrificans OS217  Bacteria  hitchhiker  0.0000566108  n/a   
 
 
-
 
NC_011883  Ddes_2112  methyl-accepting chemotaxis sensory transducer  33.33 
 
 
775 aa  119  7.999999999999999e-26  Desulfovibrio desulfuricans subsp. desulfuricans str. ATCC 27774  Bacteria  normal  n/a   
 
 
-
 
NC_009997  Sbal195_0827  methyl-accepting chemotaxis sensory transducer  33.92 
 
 
529 aa  119  9.999999999999999e-26  Shewanella baltica OS195  Bacteria  normal  normal  0.456094 
 
 
-
 
NC_002939  GSU1374  methyl-accepting chemotaxis protein  32.76 
 
 
541 aa  118  1.9999999999999998e-25  Geobacter sulfurreducens PCA  Bacteria  normal  n/a   
 
 
-
 
NC_009831  Ssed_4404  methyl-accepting chemotaxis sensory transducer  29.9 
 
 
638 aa  119  1.9999999999999998e-25  Shewanella sediminis HAW-EB3  Bacteria  normal  normal  0.0156105 
 
 
-
 
NC_013501  Rmar_0343  methyl-accepting chemotaxis sensory transducer  32.31 
 
 
1079 aa  118  1.9999999999999998e-25  Rhodothermus marinus DSM 4252  Bacteria  normal  n/a   
 
 
-
 
NC_009665  Shew185_0795  methyl-accepting chemotaxis sensory transducer  33.92 
 
 
529 aa  119  1.9999999999999998e-25  Shewanella baltica OS185  Bacteria  normal  n/a   
 
 
-
 
NC_009052  Sbal_3564  methyl-accepting chemotaxis sensory transducer  33.92 
 
 
529 aa  119  1.9999999999999998e-25  Shewanella baltica OS155  Bacteria  normal  n/a   
 
 
-
 
NC_011663  Sbal223_0820  methyl-accepting chemotaxis sensory transducer  33.92 
 
 
529 aa  118  1.9999999999999998e-25  Shewanella baltica OS223  Bacteria  normal  hitchhiker  0.00000000219782 
 
 
-
 
NC_011146  Gbem_0809  methyl-accepting chemotaxis sensory transducer  35 
 
 
532 aa  118  3e-25  Geobacter bemidjiensis Bem  Bacteria  normal  0.826751  n/a   
 
 
-
 
NC_009654  Mmwyl1_0219  methyl-accepting chemotaxis sensory transducer  36.67 
 
 
773 aa  118  3e-25  Marinomonas sp. MWYL1  Bacteria  normal  0.952357  normal 
 
 
-
 
NC_009483  Gura_3045  methyl-accepting chemotaxis sensory transducer  33.13 
 
 
563 aa  118  3e-25  Geobacter uraniireducens Rf4  Bacteria  hitchhiker  0.0000317109  n/a   
 
 
-
 
NC_008346  Swol_0971  putative methyl-accepting chemotaxis sensory transducer  28.13 
 
 
777 aa  117  3e-25  Syntrophomonas wolfei subsp. wolfei str. Goettingen  Bacteria  normal  0.271758  n/a   
 
 
-
 
NC_010511  M446_0394  methyl-accepting chemotaxis sensory transducer  32.2 
 
 
452 aa  117  3.9999999999999997e-25  Methylobacterium sp. 4-46  Bacteria  normal  normal  0.716667 
 
 
-
 
NC_011769  DvMF_1372  methyl-accepting chemotaxis sensory transducer with Pas/Pac sensor  37.32 
 
 
811 aa  117  3.9999999999999997e-25  Desulfovibrio vulgaris str. 'Miyazaki F'  Bacteria  n/a    normal 
 
 
-
 
NC_009654  Mmwyl1_0685  methyl-accepting chemotaxis sensory transducer  33.33 
 
 
620 aa  117  3.9999999999999997e-25  Marinomonas sp. MWYL1  Bacteria  normal  normal 
 
 
-
 
NC_008322  Shewmr7_1313  methyl-accepting chemotaxis sensory transducer  34.43 
 
 
667 aa  117  3.9999999999999997e-25  Shewanella sp. MR-7  Bacteria  decreased coverage  0.00146838  normal 
 
 
-
 
NC_008577  Shewana3_1293  methyl-accepting chemotaxis sensory transducer  34.43 
 
 
667 aa  117  3.9999999999999997e-25  Shewanella sp. ANA-3  Bacteria  normal  normal 
 
 
-
 
NC_008321  Shewmr4_1243  methyl-accepting chemotaxis sensory transducer  34.43 
 
 
667 aa  117  5e-25  Shewanella sp. MR-4  Bacteria  normal  normal 
 
 
-
 
NC_008345  Sfri_0080  methyl-accepting chemotaxis sensory transducer  30.58 
 
 
636 aa  117  5e-25  Shewanella frigidimarina NCIMB 400  Bacteria  normal  n/a   
 
 
-
 
NC_010506  Swoo_0808  methyl-accepting chemotaxis sensory transducer  33.88 
 
 
541 aa  117  6e-25  Shewanella woodyi ATCC 51908  Bacteria  normal  normal 
 
 
-
 
NC_010322  PputGB1_4027  methyl-accepting chemotaxis sensory transducer with Pas/Pac sensor  34.63 
 
 
521 aa  116  6.9999999999999995e-25  Pseudomonas putida GB-1  Bacteria  normal  0.664053  normal  0.0821551 
 
 
-
 
NC_012918  GM21_3453  methyl-accepting chemotaxis sensory transducer  36.07 
 
 
532 aa  116  7.999999999999999e-25  Geobacter sp. M21  Bacteria  n/a    normal  0.0235494 
 
 
-
 
NC_008577  Shewana3_2017  methyl-accepting chemotaxis sensory transducer  32.79 
 
 
543 aa  116  7.999999999999999e-25  Shewanella sp. ANA-3  Bacteria  normal  0.75882  hitchhiker  0.00000661881 
 
 
-
 
NC_012918  GM21_0039  methyl-accepting chemotaxis sensory transducer  30.43 
 
 
515 aa  115  1.0000000000000001e-24  Geobacter sp. M21  Bacteria  n/a    normal  0.0381098 
 
 
-
 
NC_013501  Rmar_2782  methyl-accepting chemotaxis sensory transducer  33.14 
 
 
601 aa  115  1.0000000000000001e-24  Rhodothermus marinus DSM 4252  Bacteria  normal  0.163256  n/a   
 
 
-
 
NC_010506  Swoo_0164  methyl-accepting chemotaxis sensory transducer with Pas/Pac sensor  36.12 
 
 
1123 aa  116  1.0000000000000001e-24  Shewanella woodyi ATCC 51908  Bacteria  normal  normal 
 
 
-
 
NC_009092  Shew_0111  methyl-accepting chemotaxis sensory transducer with Pas/Pac sensor  36.56 
 
 
864 aa  116  1.0000000000000001e-24  Shewanella loihica PV-4  Bacteria  normal  normal 
 
 
-
 
NC_010501  PputW619_3813  methyl-accepting chemotaxis sensory transducer with Pas/Pac sensor  33.57 
 
 
521 aa  116  1.0000000000000001e-24  Pseudomonas putida W619  Bacteria  normal  0.629648  normal  0.0156802 
 
 
-
 
NC_007954  Sden_0099  chemotaxis sensory transducer  30.58 
 
 
639 aa  115  1.0000000000000001e-24  Shewanella denitrificans OS217  Bacteria  unclonable  0.0000000152719  n/a   
 
 
-
 
NC_007958  RPD_3907  chemotaxis sensory transducer  35.77 
 
 
424 aa  116  1.0000000000000001e-24  Rhodopseudomonas palustris BisB5  Bacteria  normal  normal  0.827608 
 
 
-
 
NC_004347  SO_4454  methyl-accepting chemotaxis protein  31.19 
 
 
642 aa  115  2.0000000000000002e-24  Shewanella oneidensis MR-1  Bacteria  n/a    n/a   
 
 
-
 
NC_007492  Pfl01_1833  chemotaxis sensory transducer, Pas/Pac sensor  34.39 
 
 
521 aa  115  2.0000000000000002e-24  Pseudomonas fluorescens Pf0-1  Bacteria  normal  0.884809  normal 
 
 
-
 
NC_008700  Sama_2607  putative methyl-accepting chemotaxis sensory transducer  34.63 
 
 
541 aa  115  2.0000000000000002e-24  Shewanella amazonensis SB2B  Bacteria  hitchhiker  0.00122932  normal 
 
 
-
 
NC_008609  Ppro_3465  methyl-accepting chemotaxis sensory transducer  36.82 
 
 
423 aa  115  2.0000000000000002e-24  Pelobacter propionicus DSM 2379  Bacteria  normal  n/a   
 
 
-
 
NC_009512  Pput_1390  methyl-accepting chemotaxis sensory transducer  34.63 
 
 
534 aa  115  2.0000000000000002e-24  Pseudomonas putida F1  Bacteria  normal  normal 
 
 
-
 
NC_008322  Shewmr7_3750  methyl-accepting chemotaxis sensory transducer  32.77 
 
 
638 aa  115  2.0000000000000002e-24  Shewanella sp. MR-7  Bacteria  normal  0.666265  normal  0.186245 
 
 
-
 
NC_009457  VC0395_A2016  methyl-accepting chemotaxis protein  33.46 
 
 
433 aa  115  2.0000000000000002e-24  Vibrio cholerae O395  Bacteria  hitchhiker  0.0000104003  n/a   
 
 
-
 
NC_002947  PP_4521  aerotaxis receptor, putative  34.28 
 
 
521 aa  114  3e-24  Pseudomonas putida KT2440  Bacteria  normal  normal 
 
 
-
 
NC_011146  Gbem_2857  methyl-accepting chemotaxis sensory transducer  30.7 
 
 
392 aa  114  3e-24  Geobacter bemidjiensis Bem  Bacteria  normal  0.0205381  n/a   
 
 
-
 
NC_004347  SO_3282  methyl-accepting chemotaxis protein  30.58 
 
 
667 aa  114  3e-24  Shewanella oneidensis MR-1  Bacteria  n/a    n/a   
 
 
-
 
NC_013173  Dbac_0026  methyl-accepting chemotaxis sensory transducer  32.72 
 
 
686 aa  114  3e-24  Desulfomicrobium baculatum DSM 4028  Bacteria  normal  n/a   
 
 
-
 
NC_009901  Spea_3491  methyl-accepting chemotaxis sensory transducer with Cache sensor  34.11 
 
 
626 aa  114  3e-24  Shewanella pealeana ATCC 700345  Bacteria  normal  n/a   
 
 
-
 
NC_011146  Gbem_3600  methyl-accepting chemotaxis sensory transducer  32.28 
 
 
546 aa  114  3e-24  Geobacter bemidjiensis Bem  Bacteria  normal  n/a   
 
 
-
 
NC_011894  Mnod_6046  methyl-accepting chemotaxis sensory transducer  36.87 
 
 
452 aa  114  3e-24  Methylobacterium nodulans ORS 2060  Bacteria  normal  0.890066  n/a   
 
 
-
 
NC_011313  VSAL_II1022  methyl-accepting chemotaxis protein  34.51 
 
 
628 aa  115  3e-24  Aliivibrio salmonicida LFI1238  Bacteria  normal  0.771007  n/a   
 
 
-
 
NC_007908  Rfer_2350  methyl-accepting chemotaxis sensory transducer  34.02 
 
 
515 aa  114  3e-24  Rhodoferax ferrireducens T118  Bacteria  normal  n/a   
 
 
-
 
NC_008321  Shewmr4_0271  methyl-accepting chemotaxis sensory transducer  32.77 
 
 
638 aa  114  3e-24  Shewanella sp. MR-4  Bacteria  normal  0.758035  hitchhiker  0.0000678809 
 
 
-
 
NC_009092  Shew_0085  methyl-accepting chemotaxis sensory transducer  30.82 
 
 
638 aa  114  3e-24  Shewanella loihica PV-4  Bacteria  normal  0.0647334  normal 
 
 
-
 
NC_008577  Shewana3_0272  methyl-accepting chemotaxis sensory transducer  32.77 
 
 
638 aa  114  3e-24  Shewanella sp. ANA-3  Bacteria  decreased coverage  0.00090491  hitchhiker  0.000000349734 
 
 
-
 
NC_010506  Swoo_4186  methyl-accepting chemotaxis sensory transducer with Pas/Pac sensor  34.02 
 
 
526 aa  114  3e-24  Shewanella woodyi ATCC 51908  Bacteria  normal  normal 
 
 
-
 
NC_011901  Tgr7_0157  methyl-accepting chemotaxis sensory transducer  33 
 
 
646 aa  114  4.0000000000000004e-24  Thioalkalivibrio sp. HL-EbGR7  Bacteria  normal  0.805637  n/a   
 
 
-
 
NC_011830  Dhaf_2112  methyl-accepting chemotaxis sensory transducer  38.07 
 
 
571 aa  114  4.0000000000000004e-24  Desulfitobacterium hafniense DCB-2  Bacteria  normal  n/a   
 
 
-
 
NC_009784  VIBHAR_06314  histidine kinase  35.14 
 
 
623 aa  114  4.0000000000000004e-24  Vibrio harveyi ATCC BAA-1116  Bacteria  n/a    n/a   
 
 
-
 
NC_008463  PA14_30820  putative methyl-accepting chemotaxis transducer  34.12 
 
 
535 aa  114  4.0000000000000004e-24  Pseudomonas aeruginosa UCBPP-PA14  Bacteria  normal  0.0142633  hitchhiker  0.0000000000042709 
 
 
-
 
NC_008463  PA14_56000  chemotactic transducer PctA  33.73 
 
 
629 aa  114  4.0000000000000004e-24  Pseudomonas aeruginosa UCBPP-PA14  Bacteria  normal  0.0512673  normal 
 
 
-
 
NC_013173  Dbac_2646  methyl-accepting chemotaxis sensory transducer  32.35 
 
 
625 aa  114  5e-24  Desulfomicrobium baculatum DSM 4028  Bacteria  normal  n/a   
 
 
-
 
NC_003910  CPS_4372  methyl-accepting chemotaxis protein  33.88 
 
 
619 aa  114  5e-24  Colwellia psychrerythraea 34H  Bacteria  normal  n/a   
 
 
-
 
NC_007298  Daro_2834  histidine kinase, HAMP region: chemotaxis sensory transducer  39.11 
 
 
671 aa  114  5e-24  Dechloromonas aromatica RCB  Bacteria  normal  0.188903  normal 
 
 
-
 
NC_009438  Sputcn32_2422  methyl-accepting chemotaxis sensory transducer  32.48 
 
 
484 aa  114  5e-24  Shewanella putrefaciens CN-32  Bacteria  normal  n/a   
 
 
-
 
NC_010506  Swoo_3576  methyl-accepting chemotaxis sensory transducer  34.48 
 
 
772 aa  114  5e-24  Shewanella woodyi ATCC 51908  Bacteria  normal  normal  0.0207298 
 
 
-
 
NC_009438  Sputcn32_3017  methyl-accepting chemotaxis sensory transducer  32.13 
 
 
630 aa  114  5e-24  Shewanella putrefaciens CN-32  Bacteria  normal  n/a   
 
 
-
 
NC_008043  TM1040_3177  methyl-accepting chemotaxis sensory transducer  33 
 
 
749 aa  114  5e-24  Ruegeria sp. TM1040  Bacteria  normal  0.726311  normal 
 
 
-
 
NC_008740  Maqu_1216  methyl-accepting chemotaxis sensory transducer  27.93 
 
 
452 aa  114  6e-24  Marinobacter aquaeolei VT8  Bacteria  normal  0.133682  n/a   
 
 
-
 
NC_008345  Sfri_0581  methyl-accepting chemotaxis sensory transducer  33.46 
 
 
554 aa  113  6e-24  Shewanella frigidimarina NCIMB 400  Bacteria  normal  n/a   
 
 
-
 
NC_009485  BBta_6950  putative methyl-accepting chemotaxis receptor/sensory transducer  37.75 
 
 
661 aa  113  7.000000000000001e-24  Bradyrhizobium sp. BTAi1  Bacteria  normal  normal 
 
 
-
 
NC_011312  VSAL_I2193  methyl-accepting chemotaxis protein  34.93 
 
 
697 aa  113  7.000000000000001e-24  Aliivibrio salmonicida LFI1238  Bacteria  normal  n/a   
 
 
-
 
NC_009483  Gura_3311  methyl-accepting chemotaxis sensory transducer  33.7 
 
 
547 aa  113  7.000000000000001e-24  Geobacter uraniireducens Rf4  Bacteria  normal  0.691655  n/a   
 
 
-
 
NC_011663  Sbal223_3725  methyl-accepting chemotaxis sensory transducer with Pas/Pac sensor  34.11 
 
 
524 aa  113  8.000000000000001e-24  Shewanella baltica OS223  Bacteria  normal  normal 
 
 
-
 
NC_009831  Ssed_0184  methyl-accepting chemotaxis sensory transducer with PAS/Pac sensor  36.12 
 
 
863 aa  113  8.000000000000001e-24  Shewanella sediminis HAW-EB3  Bacteria  normal  normal 
 
 
-
 
NC_009052  Sbal_0543  methyl-accepting chemotaxis sensory transducer  34.11 
 
 
524 aa  113  8.000000000000001e-24  Shewanella baltica OS155  Bacteria  normal  n/a   
 
 
-
 
NC_009901  Spea_2471  methyl-accepting chemotaxis sensory transducer  34.48 
 
 
499 aa  113  8.000000000000001e-24  Shewanella pealeana ATCC 700345  Bacteria  normal  n/a   
 
 
-
 
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