More than 300 homologs were found in PanDaTox collection
for query gene Slin_2657 on replicon NC_013730
Organism: Spirosoma linguale DSM 74



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Replicon accession
Locus tag
Product
% identity
Alignment on query gene
Alignment on homolog gene
Length
Bit score

E-value
Organism name
Kingdom
Plasmid clonability
Plasmid unclonability p-value
Fosmid clonability
Fosmid unclonability p-value
Coverage Plot
PanDaTox homologs
Experimental validation
NC_013730  Slin_2657  response regulator receiver protein  100 
 
 		 128 aa  260  4.999999999999999e-69  Spirosoma linguale DSM 74  Bacteria  normal  0.0140925  normal  0.150861 
 
 
-
 
NC_013730  Slin_1037  response regulator receiver protein  51.26 
 
 		 247 aa  127  4.0000000000000003e-29  Spirosoma linguale DSM 74  Bacteria  normal  1  normal  1 
 
 
-
 
NC_010571  Oter_0804  two component LuxR family transcriptional regulator  43.8 
 
 		 204 aa  119  9.999999999999999e-27  Opitutus terrae PB90-1  Bacteria  normal  1  normal  1 
 
 
-
 
NC_013730  Slin_1040  response regulator receiver sensor signal transduction histidine kinase  46.28 
 
 		 365 aa  117  4.9999999999999996e-26  Spirosoma linguale DSM 74  Bacteria  normal  1  normal  1 
 
 
-
 
NC_011729  PCC7424_2022  response regulator receiver modulated diguanylate cyclase/phosphodiesterase  46.28 
 
 		 589 aa  110  5e-24  Cyanothece sp. PCC 7424  Bacteria  n/a    normal  1 
 
 
-
 
NC_007413  Ava_0649  response regulator receiver Signal transduction histidine kinase  43.55 
 
 		 387 aa  109  1.0000000000000001e-23  Anabaena variabilis ATCC 29413  Bacteria  normal  0.736737  normal  0.226718 
 
 
-
 
NC_014248  Aazo_0463  response regulator receiver modulated diguanylate phosphodiesterase  44.26 
 
 		 403 aa  104  3e-22  'Nostoc azollae' 0708  Bacteria  normal  1  n/a   
 
 
-
 
NC_007413  Ava_0648  diguanylate phosphodiesterase  44.54 
 
 		 403 aa  104  5e-22  Anabaena variabilis ATCC 29413  Bacteria  normal  1  normal  0.215101 
 
 
-
 
NC_011884  Cyan7425_0584  response regulator receiver protein  44.63 
 
 		 216 aa  102  2e-21  Cyanothece sp. PCC 7425  Bacteria  normal  0.0767221  normal  1 
 
 
-
 
NC_007413  Ava_4889  two component AraC family transcriptional regulator  45.24 
 
 		 288 aa  101  3e-21  Anabaena variabilis ATCC 29413  Bacteria  normal  0.298891  hitchhiker  0.00503781 
 
 
-
 
NC_008255  CHU_1132  transcriptional regulator  44.07 
 
 		 355 aa  101  3e-21  Cytophaga hutchinsonii ATCC 33406  Bacteria  normal  1  normal  0.99008 
 
 
-
 
NC_013037  Dfer_5316  transcriptional regulator, Crp/Fnr family  39.02 
 
 		 354 aa  101  4e-21  Dyadobacter fermentans DSM 18053  Bacteria  normal  1  normal  0.100222 
 
 
-
 
NC_013730  Slin_1271  transcriptional regulator, Crp/Fnr family  44.07 
 
 		 350 aa  99.4  1e-20  Spirosoma linguale DSM 74  Bacteria  normal  1  normal  1 
 
 
-
 
NC_013132  Cpin_5556  transcriptional regulator, Crp/Fnr family  40.34 
 
 		 352 aa  99.8  1e-20  Chitinophaga pinensis DSM 2588  Bacteria  normal  0.487576  normal  0.0420739 
 
 
-
 
NC_007413  Ava_0065  response regulator receiver domain-containing protein  41.32 
 
 		 196 aa  98.6  3e-20  Anabaena variabilis ATCC 29413  Bacteria  normal  0.670969  normal  0.0252366 
 
 
-
 
NC_013061  Phep_2492  response regulator receiver  40 
 
 		 352 aa  97.4  5e-20  Pedobacter heparinus DSM 2366  Bacteria  normal  0.570528  normal  0.0101485 
 
 
-
 
NC_013730  Slin_3553  transcriptional regulator, Crp/Fnr family  40.68 
 
 		 348 aa  94.7  4e-19  Spirosoma linguale DSM 74  Bacteria  normal  1  normal  0.528491 
 
 
-
 
NC_011729  PCC7424_2267  two component transcriptional regulator, AraC family  35.54 
 
 		 265 aa  92  2e-18  Cyanothece sp. PCC 7424  Bacteria  n/a    hitchhiker  0.00808217 
 
 
-
 
NC_014248  Aazo_3807  AraC family two component transcriptional regulator  38.4 
 
 		 289 aa  90.1  9e-18  'Nostoc azollae' 0708  Bacteria  normal  0.536318  n/a   
 
 
-
 
NC_013132  Cpin_3979  two component transcriptional regulator, LuxR family  32.77 
 
 		 220 aa  89.7  1e-17  Chitinophaga pinensis DSM 2588  Bacteria  normal  0.487116  normal  0.704163 
 
 
-
 
NC_013132  Cpin_2180  two component transcriptional regulator, LuxR family  33.6 
 
 		 222 aa  89.4  2e-17  Chitinophaga pinensis DSM 2588  Bacteria  normal  1  normal  0.384012 
 
 
-
 
NC_013037  Dfer_5352  two component transcriptional regulator, winged helix family  40.16 
 
 		 224 aa  88.6  3e-17  Dyadobacter fermentans DSM 18053  Bacteria  normal  1  normal  1 
 
 
-
 
NC_010718  Nther_0251  response regulator receiver protein  36.13 
 
 		 126 aa  85.5  2e-16  Natranaerobius thermophilus JW/NM-WN-LF  Bacteria  normal  1  normal  0.102048 
 
 
-
 
NC_012856  Rpic12D_2528  two component transcriptional regulator, LuxR family  36.72 
 
 		 201 aa  83.2  0.000000000000001  Ralstonia pickettii 12D  Bacteria  normal  1  normal  0.0341229 
 
 
-
 
NC_010683  Rpic_4984  two component transcriptional regulator, LuxR family  36.72 
 
 		 201 aa  83.2  0.000000000000001  Ralstonia pickettii 12J  Bacteria  normal  1  normal  0.0145131 
 
 
-
 
NC_007954  Sden_1699  ATP-binding region, ATPase-like protein  35.77 
 
 		 1396 aa  82  0.000000000000002  Shewanella denitrificans OS217  Bacteria  normal  0.170141  n/a   
 
 
-
 
NC_012850  Rleg_3564  response regulator receiver protein  35.83 
 
 		 122 aa  82  0.000000000000002  Rhizobium leguminosarum bv. trifolii WSM1325  Bacteria  normal  1  normal  0.0923704 
 
 
-
 
NC_011145  AnaeK_0824  response regulator receiver protein  39.13 
 
 		 122 aa  82  0.000000000000003  Anaeromyxobacter sp. K  Bacteria  normal  0.0263767  n/a   
 
 
-
 
NC_011891  A2cp1_0828  response regulator receiver protein  39.13 
 
 		 122 aa  81.6  0.000000000000003  Anaeromyxobacter dehalogenans 2CP-1  Bacteria  hitchhiker  0.0000089442  n/a   
 
 
-
 
NC_011884  Cyan7425_5215  two component transcriptional regulator, LuxR family  34.4 
 
 		 217 aa  81.6  0.000000000000003  Cyanothece sp. PCC 7425  Bacteria  normal  1  normal  1 
 
 
-
 
NC_010682  Rpic_2943  two component transcriptional regulator, LuxR family  36.72 
 
 		 201 aa  81.6  0.000000000000003  Ralstonia pickettii 12J  Bacteria  normal  1  normal  1 
 
 
-
 
NC_010682  Rpic_1673  two component transcriptional regulator, LuxR family  36.72 
 
 		 201 aa  81.6  0.000000000000003  Ralstonia pickettii 12J  Bacteria  normal  0.619826  normal  1 
 
 
-
 
NC_010338  Caul_0611  response regulator receiver protein  35.54 
 
 		 121 aa  80.5  0.000000000000006  Caulobacter sp. K31  Bacteria  normal  1  normal  1 
 
 
-
 
NC_007974  Rmet_5323  transcriptional regulator two component heavy metal regulatory response ZniR  37.6 
 
 		 206 aa  80.5  0.000000000000007  Cupriavidus metallidurans CH34  Bacteria  normal  1  normal  0.844748 
 
 
-
 
NC_009767  Rcas_3177  adenylate/guanylate cyclase with GAF and PAS/PAC sensors  35.04 
 
 		 971 aa  80.5  0.000000000000008  Roseiflexus castenholzii DSM 13941  Bacteria  normal  1  normal  1 
 
 
-
 
NC_010333  Caul_5319  response regulator receiver protein  35.96 
 
 		 137 aa  80.1  0.000000000000009  Caulobacter sp. K31  Bacteria  normal  0.0886883  normal  0.780288 
 
 
-
 
NC_013037  Dfer_1944  histidine kinase  36.21 
 
 		 1278 aa  80.1  0.000000000000009  Dyadobacter fermentans DSM 18053  Bacteria  normal  1  normal  0.866742 
 
 
-
 
NC_007643  Rru_A1398  response regulator receiver domain-containing protein  34.48 
 
 		 123 aa  80.1  0.00000000000001  Rhodospirillum rubrum ATCC 11170  Bacteria  normal  0.503734  n/a   
 
 
-
 
NC_007760  Adeh_0776  response regulator receiver domain-containing protein  38.26 
 
 		 122 aa  79.7  0.00000000000001  Anaeromyxobacter dehalogenans 2CP-C  Bacteria  normal  0.0195762  n/a   
 
 
-
 
NC_013422  Hneap_0134  response regulator receiver protein  35.54 
 
 		 123 aa  79.7  0.00000000000001  Halothiobacillus neapolitanus c2  Bacteria  normal  0.81317  n/a   
 
 
-
 
NC_011884  Cyan7425_1390  multi-sensor hybrid histidine kinase  33.9 
 
 		 1977 aa  79.3  0.00000000000001  Cyanothece sp. PCC 7425  Bacteria  normal  1  normal  1 
 
 
-
 
NC_011729  PCC7424_2881  two component transcriptional regulator, LuxR family  34.88 
 
 		 212 aa  79.3  0.00000000000002  Cyanothece sp. PCC 7424  Bacteria  n/a    normal  1 
 
 
-
 
NC_012918  GM21_1135  response regulator receiver protein  37.5 
 
 		 283 aa  79.3  0.00000000000002  Geobacter sp. M21  Bacteria  n/a    normal  1 
 
 
-
 
NC_011369  Rleg2_3265  response regulator receiver protein  34.17 
 
 		 122 aa  79  0.00000000000002  Rhizobium leguminosarum bv. trifolii WSM2304  Bacteria  normal  0.571434  normal  0.349593 
 
 
-
 
NC_009767  Rcas_3221  response regulator receiver modulated diguanylate cyclase  32.23 
 
 		 443 aa  79.3  0.00000000000002  Roseiflexus castenholzii DSM 13941  Bacteria  normal  1  normal  0.0497788 
 
 
-
 
NC_007604  Synpcc7942_1110  response regulator receiver domain-containing protein  31.15 
 
 		 208 aa  79.3  0.00000000000002  Synechococcus elongatus PCC 7942  Bacteria  hitchhiker  0.00277439  normal  1 
 
 
-
 
NC_007760  Adeh_1556  response regulator receiver modulated diguanylate cyclase  34.82 
 
 		 300 aa  79  0.00000000000002  Anaeromyxobacter dehalogenans 2CP-C  Bacteria  normal  1  n/a   
 
 
-
 
NC_011769  DvMF_1993  two component, sigma54 specific, transcriptional regulator, Fis family  33.61 
 
 		 486 aa  79.3  0.00000000000002  Desulfovibrio vulgaris str. 'Miyazaki F'  Bacteria  n/a    normal  1 
 
 
-
 
NC_008751  Dvul_2853  two component, sigma54 specific, Fis family transcriptional regulator  30.33 
 
 		 476 aa  79.3  0.00000000000002  Desulfovibrio vulgaris DP4  Bacteria  normal  1  normal  1 
 
 
-
 
NC_013501  Rmar_0589  response regulator receiver modulated serine phosphatase  37.29 
 
 		 371 aa  78.6  0.00000000000003  Rhodothermus marinus DSM 4252  Bacteria  normal  0.420228  n/a   
 
 
-
 
NC_013037  Dfer_1607  histidine kinase  38.84 
 
 		 1366 aa  78.6  0.00000000000003  Dyadobacter fermentans DSM 18053  Bacteria  normal  1  normal  0.89561 
 
 
-
 
NC_013132  Cpin_6438  putative two component, sigma54 specific, transcriptional regulator, Fis family  33.06 
 
 		 394 aa  78.6  0.00000000000003  Chitinophaga pinensis DSM 2588  Bacteria  decreased coverage  0.00615091  normal  0.383003 
 
 
-
 
NC_013132  Cpin_0693  two component transcriptional regulator, winged helix family  34.68 
 
 		 229 aa  78.6  0.00000000000003  Chitinophaga pinensis DSM 2588  Bacteria  normal  1  normal  1 
 
 
-
 
NC_008010  Dgeo_2844  two component transcriptional regulator  33.33 
 
 		 248 aa  78.2  0.00000000000003  Deinococcus geothermalis DSM 11300  Bacteria  normal  1  n/a   
 
 
-
 
NC_009675  Anae109_2756  response regulator receiver protein  33.61 
 
 		 136 aa  78.2  0.00000000000004  Anaeromyxobacter sp. Fw109-5  Bacteria  normal  1  normal  1 
 
 
-
 
NC_013173  Dbac_2662  two component transcriptional regulator, winged helix family  34.68 
 
 		 231 aa  77.8  0.00000000000004  Desulfomicrobium baculatum DSM 4028  Bacteria  normal  1  n/a   
 
 
-
 
NC_009485  BBta_1549  LuxR transcriptional regulator  37.6 
 
 		 201 aa  78.2  0.00000000000004  Bradyrhizobium sp. BTAi1  Bacteria  normal  0.355184  normal  0.152747 
 
 
-
 
NC_009523  RoseRS_2694  putative PAS/PAC sensor protein  34.17 
 
 		 969 aa  77.8  0.00000000000004  Roseiflexus sp. RS-1  Bacteria  normal  0.0176534  normal  1 
 
 
-
 
NC_011146  Gbem_3127  response regulator receiver protein  36.54 
 
 		 283 aa  77.8  0.00000000000005  Geobacter bemidjiensis Bem  Bacteria  normal  1  n/a   
 
 
-
 
NC_007925  RPC_4677  response regulator receiver domain-containing protein  33.06 
 
 		 121 aa  77.8  0.00000000000005  Rhodopseudomonas palustris BisB18  Bacteria  normal  1  normal  1 
 
 
-
 
NC_013522  Taci_0090  two component transcriptional regulator, winged helix family  31.09 
 
 		 228 aa  77.8  0.00000000000005  Thermanaerovibrio acidaminovorans DSM 6589  Bacteria  normal  1  n/a   
 
 
-
 
NC_011729  PCC7424_0178  response regulator receiver modulated diguanylate cyclase  36.59 
 
 		 322 aa  77.4  0.00000000000006  Cyanothece sp. PCC 7424  Bacteria  n/a    normal  0.895619 
 
 
-
 
NC_009523  RoseRS_3924  response regulator receiver modulated diguanylate cyclase  31.97 
 
 		 443 aa  77.4  0.00000000000006  Roseiflexus sp. RS-1  Bacteria  normal  0.350169  normal  1 
 
 
-
 
NC_007413  Ava_3628  two component LuxR family transcriptional regulator  32.8 
 
 		 225 aa  77  0.00000000000008  Anabaena variabilis ATCC 29413  Bacteria  normal  0.717242  normal  0.142765 
 
 
-
 
NC_014248  Aazo_2887  LuxR family two component transcriptional regulator  32.8 
 
 		 225 aa  76.6  0.00000000000009  'Nostoc azollae' 0708  Bacteria  normal  0.526109  n/a   
 
 
-
 
NC_009767  Rcas_3308  response regulator receiver protein  31.97 
 
 		 130 aa  76.6  0.0000000000001  Roseiflexus castenholzii DSM 13941  Bacteria  normal  0.678314  normal  0.215079 
 
 
-
 
NC_009675  Anae109_0442  response regulator receiver protein  38.46 
 
 		 126 aa  76.3  0.0000000000001  Anaeromyxobacter sp. Fw109-5  Bacteria  normal  0.0489543  normal  1 
 
 
-
 
NC_009943  Dole_0622  two component transcriptional regulator  32.8 
 
 		 240 aa  76.3  0.0000000000001  Desulfococcus oleovorans Hxd3  Bacteria  normal  1  n/a   
 
 
-
 
NC_009441  Fjoh_5047  two component, sigma54 specific, Fis family transcriptional regulator  36.59 
 
 		 387 aa  76.3  0.0000000000001  Flavobacterium johnsoniae UW101  Bacteria  normal  0.0897789  n/a   
 
 
-
 
NC_011729  PCC7424_3150  response regulator receiver protein  33.6 
 
 		 152 aa  75.9  0.0000000000002  Cyanothece sp. PCC 7424  Bacteria  n/a    normal  0.14204 
 
 
-
 
NC_007413  Ava_2239  adenylate/guanylate cyclase  36.75 
 
 		 1156 aa  76.3  0.0000000000002  Anabaena variabilis ATCC 29413  Bacteria  normal  0.159061  normal  1 
 
 
-
 
NC_007908  Rfer_0510  two component transcriptional regulator  37.9 
 
 		 234 aa  75.5  0.0000000000002  Rhodoferax ferrireducens T118  Bacteria  normal  1  n/a   
 
 
-
 
NC_014151  Cfla_0717  two component transcriptional regulator, winged helix family  32.77 
 
 		 242 aa  75.5  0.0000000000002  Cellulomonas flavigena DSM 20109  Bacteria  normal  1  hitchhiker  0.00000387016 
 
 
-
 
NC_013947  Snas_3775  response regulator receiver and ANTAR domain-containing protein  35.83 
 
 		 207 aa  75.9  0.0000000000002  Stackebrandtia nassauensis DSM 44728  Bacteria  normal  0.665226  normal  0.0118196 
 
 
-
 
NC_010505  Mrad2831_4812  response regulator receiver protein  29.91 
 
 		 121 aa  75.9  0.0000000000002  Methylobacterium radiotolerans JCM 2831  Bacteria  normal  1  hitchhiker  0.00197217 
 
 
-
 
NC_013730  Slin_0550  histidine kinase  36.59 
 
 		 1388 aa  76.3  0.0000000000002  Spirosoma linguale DSM 74  Bacteria  normal  0.23656  normal  0.89256 
 
 
-
 
NC_009523  RoseRS_3877  response regulator receiver protein  33.63 
 
 		 130 aa  75.5  0.0000000000002  Roseiflexus sp. RS-1  Bacteria  normal  1  normal  1 
 
 
-
 
NC_011145  AnaeK_2309  response regulator receiver modulated diguanylate cyclase  32.76 
 
 		 434 aa  74.7  0.0000000000003  Anaeromyxobacter sp. K  Bacteria  normal  0.484645  n/a   
 
 
-
 
NC_011989  Avi_4317  chemotaxis receiver protein  35.78 
 
 		 122 aa  75.1  0.0000000000003  Agrobacterium vitis S4  Bacteria  normal  0.257676  n/a   
 
 
-
 
NC_013730  Slin_2507  two component, sigma54 specific, transcriptional regulator, Fis family  35.83 
 
 		 488 aa  75.1  0.0000000000003  Spirosoma linguale DSM 74  Bacteria  normal  1  normal  0.182398 
 
 
-
 
NC_009943  Dole_1341  response regulator receiver protein  31.15 
 
 		 301 aa  75.5  0.0000000000003  Desulfococcus oleovorans Hxd3  Bacteria  normal  1  n/a   
 
 
-
 
NC_009943  Dole_2174  response regulator receiver modulated metal dependent phosphohydrolase  32.48 
 
 		 474 aa  75.1  0.0000000000003  Desulfococcus oleovorans Hxd3  Bacteria  normal  1  n/a   
 
 
-
 
NC_011891  A2cp1_2397  response regulator receiver modulated diguanylate cyclase  32.76 
 
 		 434 aa  74.7  0.0000000000004  Anaeromyxobacter dehalogenans 2CP-1  Bacteria  normal  0.593336  n/a   
 
 
-
 
NC_010814  Glov_1377  two component, sigma54 specific, transcriptional regulator, Fis family  33.62 
 
 		 462 aa  74.7  0.0000000000004  Geobacter lovleyi SZ  Bacteria  normal  0.244526  n/a   
 
 
-
 
NC_013037  Dfer_0536  histidine kinase  32.2 
 
 		 1324 aa  74.7  0.0000000000004  Dyadobacter fermentans DSM 18053  Bacteria  normal  0.0692259  normal  0.421547 
 
 
-
 
NC_010814  Glov_0588  two component transcriptional regulator, winged helix family  35.59 
 
 		 226 aa  74.7  0.0000000000004  Geobacter lovleyi SZ  Bacteria  normal  0.944339  n/a   
 
 
-
 
NC_007404  Tbd_2551  response regulator receiver domain-containing protein  38.14 
 
 		 121 aa  74.3  0.0000000000005  Thiobacillus denitrificans ATCC 25259  Bacteria  normal  1  normal  1 
 
 
-
 
NC_009675  Anae109_2257  response regulator receiver modulated diguanylate cyclase  30.77 
 
 		 509 aa  74.3  0.0000000000005  Anaeromyxobacter sp. Fw109-5  Bacteria  normal  1  normal  0.0520127 
 
 
-
 
NC_011884  Cyan7425_2947  multi-sensor signal transduction histidine kinase  30.89 
 
 		 503 aa  74.3  0.0000000000005  Cyanothece sp. PCC 7425  Bacteria  normal  1  normal  1 
 
 
-
 
NC_011365  Gdia_3466  response regulator receiver protein  34.17 
 
 		 127 aa  74.3  0.0000000000005  Gluconacetobacter diazotrophicus PAl 5  Bacteria  normal  1  normal  0.12169 
 
 
-
 
NC_008312  Tery_4323  two component LuxR family transcriptional regulator  33.88 
 
 		 215 aa  73.9  0.0000000000006  Trichodesmium erythraeum IMS101  Bacteria  normal  0.115662  normal  0.727411 
 
 
-
 
NC_013730  Slin_1735  histidine kinase  34.75 
 
 		 1343 aa  74.3  0.0000000000006  Spirosoma linguale DSM 74  Bacteria  normal  0.260049  normal  1 
 
 
-
 
NC_014248  Aazo_1477  response regulator receiver modulated diguanylate cyclase  33.33 
 
 		 317 aa  73.9  0.0000000000007  'Nostoc azollae' 0708  Bacteria  normal  1  n/a   
 
 
-
 
NC_003296  RSp0655  two component response regulator transcription regulator protein  34.17 
 
 		 228 aa  73.9  0.0000000000007  Ralstonia solanacearum GMI1000  Bacteria  normal  1  normal  0.81937 
 
 
-
 
NC_013730  Slin_4604  histidine kinase  35.83 
 
 		 663 aa  73.9  0.0000000000007  Spirosoma linguale DSM 74  Bacteria  normal  1  normal  1 
 
 
-
 
NC_011831  Cagg_1706  response regulator receiver protein  31.97 
 
 		 131 aa  73.9  0.0000000000007  Chloroflexus aggregans DSM 9485  Bacteria  normal  1  normal  1 
 
 
-
 
NC_009675  Anae109_0824  response regulator receiver protein  41.03 
 
 		 130 aa  73.6  0.0000000000008  Anaeromyxobacter sp. Fw109-5  Bacteria  normal  0.494027  normal  0.693271 
 
 
-
 
NC_007413  Ava_2324  two component LuxR family transcriptional regulator  33.06 
 
 		 245 aa  73.9  0.0000000000008  Anabaena variabilis ATCC 29413  Bacteria  normal  0.42123  normal  1 
 
 
-
 
NC_011831  Cagg_0599  response regulator receiver modulated diguanylate cyclase  28.69 
 
 		 313 aa  73.6  0.0000000000008  Chloroflexus aggregans DSM 9485  Bacteria  normal  0.464892  normal  0.548622 
 
 
-
 
NC_008688  Pden_5113  response regulator receiver protein  32.2 
 
 		 121 aa  73.6  0.0000000000008  Paracoccus denitrificans PD1222  Bacteria  normal  1  normal  1 
 
 
-
 
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