More than 300 homologs were found in PanDaTox collection
for query gene Smed_4278 on replicon NC_009620
Organism: Sinorhizobium medicae WSM419



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Replicon accession

Locus tag

Product

% identity

Alignment on query gene

Alignment on homolog gene

Length

Bit score

E-value

Organism name

Kingdom

Plasmid clonability

Plasmid unclonability p-value

Fosmid clonability

Fosmid unclonability p-value

Coverage Plot

PanDaTox homologs

Experimental validation
NC_009620  Smed_4278  putative PAS/PAC sensor protein  100 
 
 
1027 aa  2099    Sinorhizobium medicae WSM419  Bacteria  normal  normal 
 
 
-
 
NC_011004  Rpal_3739  MCP methyltransferase, CheR-type  42.51 
 
 
1045 aa  744    Rhodopseudomonas palustris TIE-1  Bacteria  normal  n/a   
 
 
-
 
NC_007908  Rfer_2355  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  44.67 
 
 
1008 aa  662    Rhodoferax ferrireducens T118  Bacteria  normal  0.71741  n/a   
 
 
-
 
NC_009622  Smed_6341  putative PAS/PAC sensor protein  70.37 
 
 
629 aa  876    Sinorhizobium medicae WSM419  Bacteria  normal  0.433344  normal  0.230755 
 
 
-
 
NC_007961  Nham_4605  MCP methyltransferase, CheR-type  39.88 
 
 
1092 aa  743    Nitrobacter hamburgensis X14  Bacteria  normal  0.315944  n/a   
 
 
-
 
NC_010625  Bphy_6399  signal transduction histidine kinase with CheB and CheR activity  42.21 
 
 
1361 aa  649    Burkholderia phymatum STM815  Bacteria  normal  0.191888  normal 
 
 
-
 
NC_010511  M446_6211  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  40.24 
 
 
1061 aa  664    Methylobacterium sp. 4-46  Bacteria  normal  0.820077  normal  0.703655 
 
 
-
 
NC_011894  Mnod_3755  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  40.36 
 
 
1063 aa  664    Methylobacterium nodulans ORS 2060  Bacteria  normal  n/a   
 
 
-
 
NC_010505  Mrad2831_5709  MCP methyltransferase, CheR-type  51.8 
 
 
853 aa  777    Methylobacterium radiotolerans JCM 2831  Bacteria  normal  normal  0.729466 
 
 
-
 
NC_009712  Mboo_0327  putative PAS/PAC sensor protein  42.23 
 
 
1008 aa  631  1e-179  Candidatus Methanoregula boonei 6A8  Archaea  normal  normal 
 
 
-
 
NC_011891  A2cp1_3377  signal transduction histidine kinase with CheB and CheR activity  43 
 
 
1242 aa  629  1e-178  Anaeromyxobacter dehalogenans 2CP-1  Bacteria  normal  n/a   
 
 
-
 
NC_007760  Adeh_3183  signal transduction histidine kinase  43.11 
 
 
1279 aa  628  1e-178  Anaeromyxobacter dehalogenans 2CP-C  Bacteria  normal  n/a   
 
 
-
 
NC_010581  Bind_0707  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  43.43 
 
 
1190 aa  627  1e-178  Beijerinckia indica subsp. indica ATCC 9039  Bacteria  normal  normal 
 
 
-
 
NC_011145  AnaeK_0372  signal transduction histidine kinase with CheB and CheR activity  43.17 
 
 
1233 aa  627  1e-178  Anaeromyxobacter sp. K  Bacteria  normal  n/a   
 
 
-
 
NC_011368  Rleg2_5237  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  41.45 
 
 
1324 aa  622  1e-176  Rhizobium leguminosarum bv. trifolii WSM2304  Bacteria  normal  normal  0.395635 
 
 
-
 
NC_007298  Daro_2045  CheB methylesterase:MCP methyltransferase, CheR-type  42.29 
 
 
868 aa  613  9.999999999999999e-175  Dechloromonas aromatica RCB  Bacteria  normal  normal 
 
 
-
 
NC_007348  Reut_B4915  PAS  40.67 
 
 
1384 aa  610  1e-173  Ralstonia eutropha JMP134  Bacteria  normal  n/a   
 
 
-
 
NC_008639  Cpha266_1788  putative PAS/PAC sensor protein  39.93 
 
 
998 aa  611  1e-173  Chlorobium phaeobacteroides DSM 266  Bacteria  normal  n/a   
 
 
-
 
NC_013173  Dbac_2162  MCP methyltransferase/methylesterase, CheR/CheB  41.37 
 
 
879 aa  608  9.999999999999999e-173  Desulfomicrobium baculatum DSM 4028  Bacteria  normal  n/a   
 
 
-
 
NC_007355  Mbar_A2183  hypothetical protein  39.35 
 
 
980 aa  605  1.0000000000000001e-171  Methanosarcina barkeri str. Fusaro  Archaea  normal  normal  0.850093 
 
 
-
 
NC_013037  Dfer_5571  signal transduction histidine kinase with CheB and CheR activity  36.52 
 
 
1218 aa  602  1e-170  Dyadobacter fermentans DSM 18053  Bacteria  normal  0.273526  normal 
 
 
-
 
NC_008709  Ping_2549  fused CheR-type MCP methyltransferase and PAS sensor protein  39.91 
 
 
1006 aa  601  1e-170  Psychromonas ingrahamii 37  Bacteria  normal  normal 
 
 
-
 
NC_011832  Mpal_2460  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  38.43 
 
 
971 aa  599  1e-170  Methanosphaerula palustris E1-9c  Archaea  normal  normal 
 
 
-
 
NC_008781  Pnap_1703  signal transduction histidine kinase with CheB and CheR activity  40.68 
 
 
1408 aa  602  1e-170  Polaromonas naphthalenivorans CJ2  Bacteria  normal  0.433463  normal 
 
 
-
 
NC_007908  Rfer_3013  MCP methyltransferase, CheR-type  41.96 
 
 
877 aa  596  1e-169  Rhodoferax ferrireducens T118  Bacteria  normal  0.404945  n/a   
 
 
-
 
NC_013132  Cpin_4422  signal transduction histidine kinase with CheB and CheR activity  39.93 
 
 
1274 aa  590  1e-167  Chitinophaga pinensis DSM 2588  Bacteria  normal  0.381764  normal 
 
 
-
 
NC_007517  Gmet_0780  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  39.86 
 
 
887 aa  592  1e-167  Geobacter metallireducens GS-15  Bacteria  normal  normal 
 
 
-
 
NC_011060  Ppha_0229  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  40.28 
 
 
1138 aa  592  1e-167  Pelodictyon phaeoclathratiforme BU-1  Bacteria  normal  n/a   
 
 
-
 
NC_011060  Ppha_1653  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  39.76 
 
 
993 aa  590  1e-167  Pelodictyon phaeoclathratiforme BU-1  Bacteria  normal  n/a   
 
 
-
 
NC_010725  Mpop_1297  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  41.38 
 
 
1168 aa  591  1e-167  Methylobacterium populi BJ001  Bacteria  normal  normal 
 
 
-
 
NC_014248  Aazo_2376  signal transduction histidine kinase with CheB and CheR activity  37.68 
 
 
1407 aa  587  1e-166  'Nostoc azollae' 0708  Bacteria  normal  n/a   
 
 
-
 
NC_007484  Noc_1601  CheB methylesterase, CheR methyltransferase, hybrid histidine kinase  40 
 
 
1222 aa  584  1.0000000000000001e-165  Nitrosococcus oceani ATCC 19707  Bacteria  normal  0.472526  n/a   
 
 
-
 
NC_010086  Bmul_3193  signal transduction histidine kinase with CheB and CheR activity  41.12 
 
 
1380 aa  583  1.0000000000000001e-165  Burkholderia multivorans ATCC 17616  Bacteria  normal  normal  0.112764 
 
 
-
 
NC_007413  Ava_0314  Signal transduction histidine kinase (STHK) with CheB and CheR activity  38 
 
 
1399 aa  581  1e-164  Anabaena variabilis ATCC 29413  Bacteria  normal  normal 
 
 
-
 
NC_013037  Dfer_5176  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  39.25 
 
 
1404 aa  580  1e-164  Dyadobacter fermentans DSM 18053  Bacteria  normal  normal  0.190634 
 
 
-
 
NC_008340  Mlg_1118  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  40.02 
 
 
852 aa  581  1e-164  Alkalilimnicola ehrlichii MLHE-1  Bacteria  normal  normal  0.742809 
 
 
-
 
NC_010803  Clim_1600  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  38.87 
 
 
1000 aa  576  1.0000000000000001e-163  Chlorobium limicola DSM 245  Bacteria  normal  n/a   
 
 
-
 
NC_009621  Smed_5776  putative PAS/PAC sensor protein  39.48 
 
 
1160 aa  575  1.0000000000000001e-162  Sinorhizobium medicae WSM419  Bacteria  normal  0.491611  normal  0.0137055 
 
 
-
 
NC_010510  Mrad2831_6264  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  41.97 
 
 
1163 aa  575  1.0000000000000001e-162  Methylobacterium radiotolerans JCM 2831  Bacteria  normal  normal 
 
 
-
 
NC_008752  Aave_2417  signal transduction histidine kinase with CheB and CheR activity  39.59 
 
 
1535 aa  566  1e-160  Acidovorax citrulli AAC00-1  Bacteria  normal  0.117494  normal 
 
 
-
 
NC_010580  Bind_3835  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  38.61 
 
 
1167 aa  563  1.0000000000000001e-159  Beijerinckia indica subsp. indica ATCC 9039  Bacteria  normal  normal 
 
 
-
 
NC_007514  Cag_0563  putative PAS/PAC sensor protein  39.23 
 
 
1035 aa  563  1.0000000000000001e-159  Chlorobium chlorochromatii CaD3  Bacteria  normal  n/a   
 
 
-
 
NC_008709  Ping_1840  MCP methyltransferase, CheR-type  38.74 
 
 
840 aa  564  1.0000000000000001e-159  Psychromonas ingrahamii 37  Bacteria  normal  0.59391  normal  0.603716 
 
 
-
 
NC_010322  PputGB1_3018  signal transduction histidine kinase with CheB and CheR activity  38.97 
 
 
1371 aa  561  1e-158  Pseudomonas putida GB-1  Bacteria  normal  normal  0.980757 
 
 
-
 
NC_008709  Ping_3128  MCP methyltransferase, CheR-type  38.2 
 
 
840 aa  558  1e-157  Psychromonas ingrahamii 37  Bacteria  normal  normal 
 
 
-
 
NC_013173  Dbac_1410  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  37 
 
 
1120 aa  542  9.999999999999999e-153  Desulfomicrobium baculatum DSM 4028  Bacteria  normal  0.132886  n/a   
 
 
-
 
NC_007643  Rru_A1500  Signal transduction histidine kinase (STHK) with CheB and CheR activity  37.37 
 
 
1483 aa  536  1e-151  Rhodospirillum rubrum ATCC 11170  Bacteria  normal  n/a   
 
 
-
 
NC_013440  Hoch_3250  signal transduction histidine kinase with CheB and CheR activity  37.89 
 
 
1348 aa  538  1e-151  Haliangium ochraceum DSM 14365  Bacteria  normal  0.534329  normal  0.0270765 
 
 
-
 
NC_008609  Ppro_3165  signal transduction histidine kinase with CheB and CheR activity  36.7 
 
 
1306 aa  538  1e-151  Pelobacter propionicus DSM 2379  Bacteria  normal  n/a   
 
 
-
 
NC_010725  Mpop_0310  MCP methyltransferase, CheR-type  39.6 
 
 
1149 aa  530  1e-149  Methylobacterium populi BJ001  Bacteria  normal  0.512157  normal 
 
 
-
 
NC_009467  Acry_3169  signal transduction histidine kinase with CheB and CheR activity  37.86 
 
 
1445 aa  522  1e-146  Acidiphilium cryptum JF-5  Bacteria  normal  n/a   
 
 
-
 
NC_008789  Hhal_1716  MCP methyltransferase, CheR-type  38.01 
 
 
856 aa  518  1.0000000000000001e-145  Halorhodospira halophila SL1  Bacteria  normal  0.92135  n/a   
 
 
-
 
NC_007955  Mbur_0399  MCP methyltransferase, CheR-type  34.89 
 
 
820 aa  511  1e-143  Methanococcoides burtonii DSM 6242  Archaea  normal  n/a   
 
 
-
 
NC_013205  Aaci_0673  signal transduction histidine kinase with CheB and CheR activity  35.49 
 
 
1215 aa  509  1e-143  Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446  Bacteria  normal  n/a   
 
 
-
 
NC_007912  Sde_3046  hypothetical protein  36.02 
 
 
1010 aa  507  9.999999999999999e-143  Saccharophagus degradans 2-40  Bacteria  normal  hitchhiker  0.00164382 
 
 
-
 
NC_013061  Phep_3913  PAS sensor protein  35.65 
 
 
1337 aa  509  9.999999999999999e-143  Pedobacter heparinus DSM 2366  Bacteria  normal  0.290527  normal  0.28306 
 
 
-
 
NC_007493  RSP_2229  methylesterase CheB/methylase CheR  37.35 
 
 
1170 aa  496  1e-139  Rhodobacter sphaeroides 2.4.1  Bacteria  normal  0.840236  n/a   
 
 
-
 
NC_009049  Rsph17029_0905  putative PAS/PAC sensor protein  37.35 
 
 
1170 aa  496  1e-139  Rhodobacter sphaeroides ATCC 17029  Bacteria  normal  normal  0.968727 
 
 
-
 
NC_010505  Mrad2831_4430  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  39.01 
 
 
1158 aa  494  9.999999999999999e-139  Methylobacterium radiotolerans JCM 2831  Bacteria  normal  normal 
 
 
-
 
NC_009428  Rsph17025_2264  putative PAS/PAC sensor protein  37.68 
 
 
1165 aa  494  9.999999999999999e-139  Rhodobacter sphaeroides ATCC 17025  Bacteria  normal  0.620072  normal  0.0600346 
 
 
-
 
NC_007953  Bxe_C0521  multi sensor hybrid histidine kinase  37.18 
 
 
1344 aa  492  1e-137  Burkholderia xenovorans LB400  Bacteria  normal  normal 
 
 
-
 
NC_013132  Cpin_7206  signal transduction histidine kinase with CheB and CheR activity  35.82 
 
 
1499 aa  488  1e-136  Chitinophaga pinensis DSM 2588  Bacteria  normal  normal 
 
 
-
 
NC_013440  Hoch_4639  signal transduction histidine kinase with CheB and CheR activity  35.36 
 
 
1453 aa  488  1e-136  Haliangium ochraceum DSM 14365  Bacteria  normal  0.432269  normal 
 
 
-
 
NC_013440  Hoch_1404  signal transduction histidine kinase with CheB and CheR activity  36.95 
 
 
1759 aa  476  1e-132  Haliangium ochraceum DSM 14365  Bacteria  normal  normal 
 
 
-
 
NC_013440  Hoch_3278  signal transduction histidine kinase with CheB and CheR activity  36.41 
 
 
2468 aa  470  1.0000000000000001e-131  Haliangium ochraceum DSM 14365  Bacteria  normal  normal  0.0267066 
 
 
-
 
NC_013440  Hoch_5252  signal transduction histidine kinase with CheB and CheR activity  36.75 
 
 
1698 aa  472  1.0000000000000001e-131  Haliangium ochraceum DSM 14365  Bacteria  normal  normal 
 
 
-
 
NC_013730  Slin_3102  signal transduction histidine kinase with CheB and CheR activity  36.89 
 
 
1418 aa  461  9.999999999999999e-129  Spirosoma linguale DSM 74  Bacteria  normal  normal 
 
 
-
 
NC_008751  Dvul_2487  signal transduction histidine kinase with CheB and CheR activity  35.63 
 
 
1535 aa  460  9.999999999999999e-129  Desulfovibrio vulgaris DP4  Bacteria  normal  0.162467  normal  0.455724 
 
 
-
 
NC_014230  CA2559_12648  Signal Transduction Histidine Kinase (STHK) with CheB and CheRactivity  32 
 
 
1200 aa  456  1e-127  Croceibacter atlanticus HTCC2559  Bacteria  normal  n/a   
 
 
-
 
NC_007952  Bxe_B1520  signal transduction histidine kinase (STHK) with CheB and CheR activity  36.42 
 
 
1303 aa  451  1e-125  Burkholderia xenovorans LB400  Bacteria  normal  0.124967  normal 
 
 
-
 
NC_002977  MCA1246  methyltransferase CheR, putative  37.52 
 
 
1378 aa  441  9.999999999999999e-123  Methylococcus capsulatus str. Bath  Bacteria  normal  0.271052  n/a   
 
 
-
 
NC_008255  CHU_1237  chemotaxis response regulator (methyltransferase)  32.74 
 
 
1618 aa  421  1e-116  Cytophaga hutchinsonii ATCC 33406  Bacteria  normal  normal 
 
 
-
 
NC_010508  Bcenmc03_1302  signal transduction histidine kinase with CheB and CheR activity  32.21 
 
 
1220 aa  417  9.999999999999999e-116  Burkholderia cenocepacia MC0-3  Bacteria  normal  normal  0.772751 
 
 
-
 
NC_010086  Bmul_4696  signal transduction histidine kinase with CheB and CheR activity  32.91 
 
 
1214 aa  411  1e-113  Burkholderia multivorans ATCC 17616  Bacteria  normal  normal 
 
 
-
 
NC_007802  Jann_2564  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  33.8 
 
 
1089 aa  407  1.0000000000000001e-112  Jannaschia sp. CCS1  Bacteria  normal  normal 
 
 
-
 
NC_009622  Smed_6342  CheB methylesterase  69.9 
 
 
336 aa  404  1e-111  Sinorhizobium medicae WSM419  Bacteria  normal  0.582664  normal  0.191496 
 
 
-
 
NC_008044  TM1040_0460  MCP methyltransferase, CheR-type  34.19 
 
 
837 aa  398  1e-109  Ruegeria sp. TM1040  Bacteria  normal  0.604812  normal 
 
 
-
 
NC_008044  TM1040_1028  signal transduction histidine kinase with CheB and CheR activity  32.36 
 
 
1248 aa  397  1e-109  Ruegeria sp. TM1040  Bacteria  normal  normal  0.507304 
 
 
-
 
NC_008044  TM1040_2227  MCP methyltransferase, CheR-type  33 
 
 
824 aa  391  1e-107  Ruegeria sp. TM1040  Bacteria  normal  normal 
 
 
-
 
NC_008789  Hhal_0370  protein-glutamate O-methyltransferase  37.73 
 
 
639 aa  387  1e-106  Halorhodospira halophila SL1  Bacteria  normal  0.181962  n/a   
 
 
-
 
NC_007348  Reut_B5191  CheB methylesterase:MCP methyltransferase, CheR-type  31.77 
 
 
813 aa  380  1e-104  Ralstonia eutropha JMP134  Bacteria  normal  n/a   
 
 
-
 
NC_008576  Mmc1_3390  MCP methyltransferase, CheR-type  37.73 
 
 
617 aa  383  1e-104  Magnetococcus sp. MC-1  Bacteria  normal  normal 
 
 
-
 
NC_010623  Bphy_5175  MCP methyltransferase, CheR-type  30.7 
 
 
823 aa  370  1e-101  Burkholderia phymatum STM815  Bacteria  normal  normal  0.367936 
 
 
-
 
NC_013730  Slin_1989  MCP methyltransferase, CheR-type with PAS/PAC sensor  33.76 
 
 
1110 aa  362  1e-98  Spirosoma linguale DSM 74  Bacteria  normal  0.175442  normal  0.013335 
 
 
-
 
NC_010717  PXO_03023  methyltransferase  42.96 
 
 
604 aa  354  4e-96  Xanthomonas oryzae pv. oryzae PXO99A  Bacteria  normal  0.361036  n/a   
 
 
-
 
NC_010814  Glov_0566  MCP methyltransferase, CheR-type with PAS/PAC sensor  35.66 
 
 
1193 aa  350  1e-94  Geobacter lovleyi SZ  Bacteria  normal  n/a   
 
 
-
 
NC_010814  Glov_2975  diguanylate cyclase with PAS/PAC sensor  35.84 
 
 
1084 aa  348  4e-94  Geobacter lovleyi SZ  Bacteria  normal  n/a   
 
 
-
 
NC_007964  Nham_3990  MCP methyltransferase, CheR-type  40.45 
 
 
432 aa  305  2.0000000000000002e-81  Nitrobacter hamburgensis X14  Bacteria  normal  n/a   
 
 
-
 
NC_013440  Hoch_3427  MCP methyltransferase, CheR-type with PAS/PAC sensor  29.72 
 
 
2031 aa  282  3e-74  Haliangium ochraceum DSM 14365  Bacteria  normal  normal  0.0889636 
 
 
-
 
NC_013132  Cpin_3640  MCP methyltransferase, CheR-type with PAS/PAC sensor  32.47 
 
 
1303 aa  280  1e-73  Chitinophaga pinensis DSM 2588  Bacteria  normal  normal  0.581797 
 
 
-
 
NC_014248  Aazo_0227  MCP methyltransferase  31.2 
 
 
631 aa  276  1.0000000000000001e-72  'Nostoc azollae' 0708  Bacteria  normal  0.993793  n/a   
 
 
-
 
NC_011729  PCC7424_0433  MCP methyltransferase, CheR-type with PAS/PAC sensor  31.7 
 
 
618 aa  254  6e-66  Cyanothece sp. PCC 7424  Bacteria  n/a    normal 
 
 
-
 
NC_011884  Cyan7425_3121  MCP methyltransferase, CheR-type with PAS/PAC sensor  32.28 
 
 
617 aa  245  3.9999999999999997e-63  Cyanothece sp. PCC 7425  Bacteria  normal  0.0152355  normal  0.805919 
 
 
-
 
NC_009485  BBta_6552  signal transduction histidine kinase  40.79 
 
 
489 aa  169  2e-40  Bradyrhizobium sp. BTAi1  Bacteria  normal  normal  0.433672 
 
 
-
 
NC_009712  Mboo_1581  protein-glutamate O-methyltransferase  39.68 
 
 
269 aa  169  2.9999999999999998e-40  Candidatus Methanoregula boonei 6A8  Archaea  normal  0.175681  normal  0.125652 
 
 
-
 
NC_007488  RSP_3982  hypothetical protein  37.61 
 
 
478 aa  169  2.9999999999999998e-40  Rhodobacter sphaeroides 2.4.1  Bacteria  normal  n/a   
 
 
-
 
NC_012858  Rleg_6933  signal transduction histidine kinase  33.55 
 
 
488 aa  166  3e-39  Rhizobium leguminosarum bv. trifolii WSM1325  Bacteria  normal  0.0223762  normal 
 
 
-
 
NC_009429  Rsph17025_3313  hypothetical protein  32.66 
 
 
480 aa  164  9e-39  Rhodobacter sphaeroides ATCC 17025  Bacteria  normal  normal  0.29262 
 
 
-
 
NC_011894  Mnod_0965  signal transduction histidine kinase  40.64 
 
 
850 aa  160  1e-37  Methylobacterium nodulans ORS 2060  Bacteria  normal  n/a   
 
 
-
 
NC_010172  Mext_1881  PAS sensor protein  37.27 
 
 
872 aa  158  5.0000000000000005e-37  Methylobacterium extorquens PA1  Bacteria  normal  normal 
 
 
-
 
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