More than 300 homologs were found in PanDaTox collection
for query gene Mflv_3054 on replicon NC_009338
Organism: Mycobacterium gilvum PYR-GCK



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Replicon accession
Locus tag
Product
% identity
Alignment on query gene
Alignment on homolog gene
Length
Bit score

E-value
Organism name
Kingdom
Plasmid clonability
Plasmid unclonability p-value
Fosmid clonability
Fosmid unclonability p-value
Coverage Plot
PanDaTox homologs
Experimental validation
NC_009338  Mflv_3054  HAD family hydrolase  100 
 
 		 224 aa  450  1.0000000000000001e-126  Mycobacterium gilvum PYR-GCK  Bacteria  normal  0.751068  normal  0.223571 
 
 
-
 
NC_008726  Mvan_3473  HAD family hydrolase  82.59 
 
 		 243 aa  366  1e-100  Mycobacterium vanbaalenii PYR-1  Bacteria  normal  1  normal  1 
 
 
-
 
NC_009077  Mjls_3160  HAD family hydrolase  67.56 
 
 		 228 aa  274  6e-73  Mycobacterium sp. JLS  Bacteria  normal  0.0720917  normal  0.533727 
 
 
-
 
NC_008146  Mmcs_3148  HAD family hydrolase  67.56 
 
 		 228 aa  274  6e-73  Mycobacterium sp. MCS  Bacteria  normal  0.0274068  n/a   
 
 
-
 
NC_008705  Mkms_3210  HAD family hydrolase  67.56 
 
 		 228 aa  274  6e-73  Mycobacterium sp. KMS  Bacteria  normal  0.742759  normal  0.278604 
 
 
-
 
NC_013093  Amir_2212  HAD-superfamily hydrolase, subfamily IA, variant 3  51.74 
 
 		 220 aa  199  3e-50  Actinosynnema mirum DSM 43827  Bacteria  normal  1  n/a   
 
 
-
 
NC_013947  Snas_4498  HAD-superfamily hydrolase subfamily IA, variant 3  45.45 
 
 		 223 aa  174  9.999999999999999e-43  Stackebrandtia nassauensis DSM 44728  Bacteria  normal  0.511661  normal  0.0143777 
 
 
-
 
NC_013159  Svir_22200  haloacid dehalogenase superfamily protein, subfamily IA, variant 3 with third motif having DD or ED  47.76 
 
 		 217 aa  168  6e-41  Saccharomonospora viridis DSM 43017  Bacteria  normal  0.172111  normal  1 
 
 
-
 
NC_013441  Gbro_2452  HAD-superfamily hydrolase, subfamily IA, variant 3  47.62 
 
 		 232 aa  167  2e-40  Gordonia bronchialis DSM 43247  Bacteria  normal  1  n/a   
 
 
-
 
NC_014158  Tpau_2146  HAD-superfamily hydrolase, subfamily IA, variant 3  42.65 
 
 		 213 aa  160  1e-38  Tsukamurella paurometabola DSM 20162  Bacteria  normal  1  n/a   
 
 
-
 
NC_013235  Namu_2787  HAD-superfamily hydrolase, subfamily IA, variant 3  44.24 
 
 		 230 aa  154  1e-36  Nakamurella multipartita DSM 44233  Bacteria  hitchhiker  0.00137861  hitchhiker  0.0000132599 
 
 
-
 
NC_009953  Sare_2349  HAD family hydrolase  48.68 
 
 		 215 aa  143  2e-33  Salinispora arenicola CNS-205  Bacteria  normal  1  normal  0.355354 
 
 
-
 
NC_009664  Krad_1843  HAD-superfamily hydrolase, subfamily IA, variant 3  41.81 
 
 		 241 aa  142  4e-33  Kineococcus radiotolerans SRS30216  Bacteria  normal  0.101722  normal  1 
 
 
-
 
NC_012669  Bcav_2245  HAD-superfamily hydrolase, subfamily IA, variant 3  41.31 
 
 		 247 aa  138  8.999999999999999e-32  Beutenbergia cavernae DSM 12333  Bacteria  normal  0.56873  normal  1 
 
 
-
 
NC_009380  Strop_2232  HAD family hydrolase  46.56 
 
 		 233 aa  132  3.9999999999999996e-30  Salinispora tropica CNB-440  Bacteria  normal  0.470588  normal  1 
 
 
-
 
NC_014165  Tbis_1853  HAD-superfamily hydrolase  39.15 
 
 		 213 aa  127  2.0000000000000002e-28  Thermobispora bispora DSM 43833  Bacteria  normal  1  normal  1 
 
 
-
 
NC_008699  Noca_2632  HAD family hydrolase  38.94 
 
 		 218 aa  123  2e-27  Nocardioides sp. JS614  Bacteria  normal  1  n/a   
 
 
-
 
NC_013131  Caci_2412  HAD-superfamily hydrolase, subfamily IA, variant 3  36.92 
 
 		 222 aa  123  2e-27  Catenulispora acidiphila DSM 44928  Bacteria  normal  1  normal  1 
 
 
-
 
NC_013595  Sros_5916  HAD family hydrolase  36.04 
 
 		 218 aa  120  9.999999999999999e-27  Streptosporangium roseum DSM 43021  Bacteria  normal  0.0345843  normal  0.0351614 
 
 
-
 
NC_013521  Sked_20940  haloacid dehalogenase superfamily enzyme, subfamily IA  35.75 
 
 		 273 aa  119  3.9999999999999996e-26  Sanguibacter keddieii DSM 10542  Bacteria  normal  1  normal  0.650807 
 
 
-
 
NC_007777  Francci3_2637  HAD family hydrolase  37.43 
 
 		 286 aa  117  9.999999999999999e-26  Frankia sp. CcI3  Bacteria  normal  0.403398  normal  0.292856 
 
 
-
 
NC_013595  Sros_7385  HAD-superfamily hydrolase, subfamily IA  35.65 
 
 		 248 aa  115  3.9999999999999997e-25  Streptosporangium roseum DSM 43021  Bacteria  normal  1  normal  1 
 
 
-
 
NC_013169  Ksed_13910  haloacid dehalogenase superfamily protein, subfamily IA, variant 3 with third motif having DD or ED  37.13 
 
 		 232 aa  114  1.0000000000000001e-24  Kytococcus sedentarius DSM 20547  Bacteria  normal  0.491881  normal  1 
 
 
-
 
NC_014210  Ndas_1550  HAD-superfamily hydrolase, subfamily IA, variant 3  39.47 
 
 		 249 aa  114  2.0000000000000002e-24  Nocardiopsis dassonvillei subsp. dassonvillei DSM 43111  Bacteria  normal  1  normal  0.204001 
 
 
-
 
NC_013757  Gobs_2673  HAD-superfamily hydrolase, subfamily IA, variant 3  40.21 
 
 		 248 aa  111  8.000000000000001e-24  Geodermatophilus obscurus DSM 43160  Bacteria  normal  0.649761  n/a   
 
 
-
 
NC_011886  Achl_1913  HAD-superfamily hydrolase, subfamily IA, variant 3  40.4 
 
 		 245 aa  110  1.0000000000000001e-23  Arthrobacter chlorophenolicus A6  Bacteria  n/a    hitchhiker  0.00000000014808 
 
 
-
 
NC_009921  Franean1_4889  HAD family hydrolase  39.36 
 
 		 233 aa  110  2.0000000000000002e-23  Frankia sp. EAN1pec  Bacteria  normal  0.351284  normal  0.0730929 
 
 
-
 
NC_008148  Rxyl_0905  HAD family hydrolase  38.98 
 
 		 204 aa  110  2.0000000000000002e-23  Rubrobacter xylanophilus DSM 9941  Bacteria  normal  0.427836  n/a   
 
 
-
 
NC_013510  Tcur_2293  HAD-superfamily hydrolase, subfamily IA, variant 3  40.78 
 
 		 218 aa  109  4.0000000000000004e-23  Thermomonospora curvata DSM 43183  Bacteria  hitchhiker  0.0000494788  n/a   
 
 
-
 
NC_007333  Tfu_1824  HAD family hydrolase  40.96 
 
 		 222 aa  108  5e-23  Thermobifida fusca YX  Bacteria  normal  1  n/a   
 
 
-
 
NC_013174  Jden_1197  HAD-superfamily hydrolase, subfamily IA, variant 3  32.21 
 
 		 225 aa  105  8e-22  Jonesia denitrificans DSM 20603  Bacteria  normal  0.0955551  normal  0.148864 
 
 
-
 
NC_011004  Rpal_4817  HAD-superfamily hydrolase, subfamily IA, variant 3  36.76 
 
 		 235 aa  104  9e-22  Rhodopseudomonas palustris TIE-1  Bacteria  normal  0.821962  n/a   
 
 
-
 
NC_007958  RPD_3933  HAD family hydrolase  35.78 
 
 		 271 aa  102  3e-21  Rhodopseudomonas palustris BisB5  Bacteria  normal  1  normal  1 
 
 
-
 
NC_008541  Arth_2170  HAD family hydrolase  36.74 
 
 		 263 aa  100  2e-20  Arthrobacter sp. FB24  Bacteria  normal  0.156461  n/a   
 
 
-
 
NC_008578  Acel_1175  HAD family hydrolase  35.98 
 
 		 263 aa  100  3e-20  Acidothermus cellulolyticus 11B  Bacteria  normal  1  normal  1 
 
 
-
 
NC_002947  PP_2052  HAD superfamily hydrolase  35.07 
 
 		 707 aa  99.4  4e-20  Pseudomonas putida KT2440  Bacteria  normal  0.380026  normal  1 
 
 
-
 
NC_013526  Tter_2485  HAD-superfamily hydrolase, subfamily IA, variant 3  35.52 
 
 		 238 aa  98.2  8e-20  Thermobaculum terrenum ATCC BAA-798  Bacteria  normal  1  n/a   
 
 
-
 
NC_013530  Xcel_1481  HAD-superfamily hydrolase, subfamily IA, variant 3  34.27 
 
 		 233 aa  98.2  9e-20  Xylanimonas cellulosilytica DSM 15894  Bacteria  normal  1  n/a   
 
 
-
 
NC_009512  Pput_3689  HAD family hydrolase  35.08 
 
 		 707 aa  98.2  1e-19  Pseudomonas putida F1  Bacteria  normal  1  normal  1 
 
 
-
 
NC_012803  Mlut_11830  haloacid dehalogenase superfamily protein, subfamily IA, variant 3 with third motif having DD or ED  38.03 
 
 		 246 aa  97.8  1e-19  Micrococcus luteus NCTC 2665  Bacteria  normal  0.0867005  n/a   
 
 
-
 
NC_007778  RPB_1286  HAD family hydrolase  34.8 
 
 		 229 aa  97.1  2e-19  Rhodopseudomonas palustris HaA2  Bacteria  normal  1  normal  1 
 
 
-
 
NC_010322  PputGB1_1571  HAD family hydrolase  35.08 
 
 		 714 aa  97.4  2e-19  Pseudomonas putida GB-1  Bacteria  normal  1  normal  0.882263 
 
 
-
 
NC_010501  PputW619_2172  HAD family hydrolase  35.21 
 
 		 710 aa  96.7  3e-19  Pseudomonas putida W619  Bacteria  normal  1  normal  1 
 
 
-
 
NC_014151  Cfla_2008  HAD-superfamily hydrolase, subfamily IA, variant 3  35.71 
 
 		 213 aa  95.9  5e-19  Cellulomonas flavigena DSM 20109  Bacteria  normal  0.296794  normal  1 
 
 
-
 
NC_007519  Dde_2523  HAD family hydrolase  34.92 
 
 		 219 aa  94.4  1e-18  Desulfovibrio desulfuricans subsp. desulfuricans str. G20  Bacteria  hitchhiker  0.000000000119514  n/a   
 
 
-
 
NC_014148  Plim_3898  HAD-superfamily hydrolase, subfamily IA, variant 3  31.52 
 
 		 233 aa  94.4  1e-18  Planctomyces limnophilus DSM 3776  Bacteria  normal  1  n/a   
 
 
-
 
NC_010581  Bind_0935  HAD family hydrolase  36.02 
 
 		 235 aa  93.6  2e-18  Beijerinckia indica subsp. indica ATCC 9039  Bacteria  normal  0.941543  normal  1 
 
 
-
 
NC_009654  Mmwyl1_2542  HAD family hydrolase  33.88 
 
 		 214 aa  93.6  2e-18  Marinomonas sp. MWYL1  Bacteria  normal  1  normal  0.214098 
 
 
-
 
NC_008044  TM1040_1460  HAD family hydrolase  35.23 
 
 		 229 aa  92.8  3e-18  Ruegeria sp. TM1040  Bacteria  hitchhiker  0.00552213  normal  0.266811 
 
 
-
 
NC_009456  VC0395_0037  haloacid dehalogenase/epoxide hydrolase family protein  33.68 
 
 		 219 aa  92.4  4e-18  Vibrio cholerae O395  Bacteria  normal  1  n/a   
 
 
-
 
NC_011661  Dtur_0461  HAD-superfamily hydrolase, subfamily IA, variant 3  31.86 
 
 		 217 aa  92.4  5e-18  Dictyoglomus turgidum DSM 6724  Bacteria  normal  1  n/a   
 
 
-
 
NC_013159  Svir_32070  haloacid dehalogenase superfamily enzyme, subfamily IA  34.59 
 
 		 227 aa  91.7  8e-18  Saccharomonospora viridis DSM 43017  Bacteria  normal  0.466176  normal  0.444114 
 
 
-
 
NC_013947  Snas_4073  HAD-superfamily hydrolase subfamily IA, variant 3  34.95 
 
 		 219 aa  91.7  9e-18  Stackebrandtia nassauensis DSM 44728  Bacteria  normal  0.358464  normal  1 
 
 
-
 
NC_009784  VIBHAR_05174  phosphoglycolate phosphatase  32.07 
 
 		 256 aa  90.9  1e-17  Vibrio harveyi ATCC BAA-1116  Bacteria  n/a    n/a   
 
 
-
 
NC_013172  Bfae_25290  haloacid dehalogenase superfamily enzyme, subfamily IA  34.15 
 
 		 244 aa  90.5  2e-17  Brachybacterium faecium DSM 4810  Bacteria  normal  0.0218735  n/a   
 
 
-
 
NC_010003  Pmob_0452  HAD family hydrolase  28.89 
 
 		 221 aa  90.1  3e-17  Petrotoga mobilis SJ95  Bacteria  normal  1  n/a   
 
 
-
 
NC_009485  BBta_1791  putative haloacid dehalogenase/epoxide hydrolase  35.64 
 
 		 218 aa  89.4  4e-17  Bradyrhizobium sp. BTAi1  Bacteria  normal  1  normal  1 
 
 
-
 
NC_009012  Cthe_0780  HAD family hydrolase  29.57 
 
 		 227 aa  89  5e-17  Clostridium thermocellum ATCC 27405  Bacteria  normal  1  n/a   
 
 
-
 
NC_007947  Mfla_1729  HAD family hydrolase  34.46 
 
 		 728 aa  89  6e-17  Methylobacillus flagellatus KT  Bacteria  normal  0.696153  hitchhiker  0.000289006 
 
 
-
 
NC_013457  VEA_001299  CbbY family protein  32.26 
 
 		 216 aa  88.2  8e-17  Vibrio sp. Ex25  Bacteria  normal  0.916657  n/a   
 
 
-
 
NC_013721  HMPREF0424_1278  HAD hydrolase, family IA, variant 3  29.69 
 
 		 247 aa  88.2  8e-17  Gardnerella vaginalis 409-05  Bacteria  n/a    normal  1 
 
 
-
 
NC_011071  Smal_0452  HAD-superfamily hydrolase, subfamily IA, variant 3  34.03 
 
 		 227 aa  88.2  1e-16  Stenotrophomonas maltophilia R551-3  Bacteria  normal  1  normal  1 
 
 
-
 
NC_013093  Amir_7029  HAD-superfamily hydrolase, subfamily IA, variant 3  34.39 
 
 		 213 aa  87.4  2e-16  Actinosynnema mirum DSM 43827  Bacteria  normal  1  n/a   
 
 
-
 
NC_010001  Cphy_2790  HAD family hydrolase  27.1 
 
 		 223 aa  86.7  3e-16  Clostridium phytofermentans ISDg  Bacteria  normal  1  n/a   
 
 
-
 
NC_007925  RPC_4133  HAD family hydrolase  36.76 
 
 		 223 aa  86.7  3e-16  Rhodopseudomonas palustris BisB18  Bacteria  normal  1  normal  1 
 
 
-
 
NC_008709  Ping_3528  HAD family hydrolase  30.96 
 
 		 222 aa  86.3  3e-16  Psychromonas ingrahamii 37  Bacteria  normal  0.894258  hitchhiker  0.00350401 
 
 
-
 
NC_011676  PHATRDRAFT_35659  predicted protein  30.77 
 
 		 267 aa  86.3  4e-16  Phaeodactylum tricornutum CCAP 1055/1  Eukaryota  normal  1  n/a   
 
 
-
 
NC_013517  Sterm_2266  HAD-superfamily hydrolase, subfamily IA, variant 3  30.85 
 
 		 220 aa  84.7  0.000000000000001  Sebaldella termitidis ATCC 33386  Bacteria  normal  1  n/a   
 
 
-
 
NC_010717  PXO_03084  HAD superfamily hydrolase  32.69 
 
 		 226 aa  83.6  0.000000000000002  Xanthomonas oryzae pv. oryzae PXO99A  Bacteria  normal  1  n/a   
 
 
-
 
NC_011772  BCG9842_B0922  hydrolase, haloacid dehalogenase-like family  30.32 
 
 		 235 aa  84  0.000000000000002  Bacillus cereus G9842  Bacteria  normal  1  normal  1 
 
 
-
 
NC_013517  Sterm_2267  HAD-superfamily hydrolase, subfamily IA, variant 3  29.79 
 
 		 219 aa  83.6  0.000000000000002  Sebaldella termitidis ATCC 33386  Bacteria  normal  1  n/a   
 
 
-
 
NC_009012  Cthe_3073  HAD family hydrolase  30.98 
 
 		 223 aa  84  0.000000000000002  Clostridium thermocellum ATCC 27405  Bacteria  hitchhiker  0.00582956  n/a   
 
 
-
 
NC_011725  BCB4264_A4313  hydrolase, haloacid dehalogenase-like family  29.79 
 
 		 235 aa  83.2  0.000000000000003  Bacillus cereus B4264  Bacteria  normal  1  n/a   
 
 
-
 
NC_005945  BAS4107  HAD superfamily hydrolase  27.27 
 
 		 221 aa  83.2  0.000000000000003  Bacillus anthracis str. Sterne  Bacteria  normal  1  n/a   
 
 
-
 
NC_007530  GBAA_4427  HAD superfamily hydrolase  27.27 
 
 		 221 aa  83.2  0.000000000000003  Bacillus anthracis str. 'Ames Ancestor'  Bacteria  normal  1  n/a   
 
 
-
 
NC_003909  BCE_4276  HAD superfamily hydrolase  28.16 
 
 		 220 aa  82.8  0.000000000000004  Bacillus cereus ATCC 10987  Bacteria  normal  1  n/a   
 
 
-
 
NC_010338  Caul_3346  HAD family hydrolase  30.69 
 
 		 221 aa  82.8  0.000000000000004  Caulobacter sp. K31  Bacteria  normal  0.363754  normal  1 
 
 
-
 
NC_007955  Mbur_1556  HAD family hydrolase  29.57 
 
 		 232 aa  82.4  0.000000000000004  Methanococcoides burtonii DSM 6242  Archaea  decreased coverage  0.0000000000353855  n/a   
 
 
-
 
NC_013421  Pecwa_1118  fructose-1-phosphatase  32.62 
 
 		 188 aa  82  0.000000000000006  Pectobacterium wasabiae WPP163  Bacteria  decreased coverage  0.000766383  n/a   
 
 
-
 
NC_005957  BT9727_3945  HAD superfamily hydrolase  26.82 
 
 		 221 aa  81.6  0.000000000000009  Bacillus thuringiensis serovar konkukian str. 97-27  Bacteria  normal  1  n/a   
 
 
-
 
NC_006274  BCZK3956  HAD superfamily hydrolase  26.82 
 
 		 220 aa  81.6  0.000000000000009  Bacillus cereus E33L  Bacteria  normal  0.945579  n/a   
 
 
-
 
NC_007413  Ava_3074  HAD family hydrolase  31.79 
 
 		 222 aa  81.6  0.000000000000009  Anabaena variabilis ATCC 29413  Bacteria  normal  1  normal  1 
 
 
-
 
NC_012912  Dd1591_0985  fructose-1-phosphatase  34.39 
 
 		 188 aa  81.3  0.00000000000001  Dickeya zeae Ech1591  Bacteria  decreased coverage  0.000117667  n/a   
 
 
-
 
NC_007404  Tbd_2229  phosphoglycolate phosphatase  31.58 
 
 		 227 aa  81.3  0.00000000000001  Thiobacillus denitrificans ATCC 25259  Bacteria  normal  0.40738  normal  1 
 
 
-
 
NC_011773  BCAH820_4222  hydrolase, haloacid dehalogenase-like family  26.82 
 
 		 220 aa  81.6  0.00000000000001  Bacillus cereus AH820  Bacteria  n/a    normal  1 
 
 
-
 
NC_011899  Hore_10160  HAD-superfamily hydrolase, subfamily IA, variant 3  28.02 
 
 		 217 aa  80.9  0.00000000000001  Halothermothrix orenii H 168  Bacteria  normal  0.64096  n/a   
 
 
-
 
NC_012917  PC1_3209  fructose-1-phosphatase  33.16 
 
 		 188 aa  81.3  0.00000000000001  Pectobacterium carotovorum subsp. carotovorum PC1  Bacteria  unclonable  0.0000636798  n/a   
 
 
-
 
NC_008819  NATL1_06771  phosphatase/phosphohexomutase  31.35 
 
 		 226 aa  81.6  0.00000000000001  Prochlorococcus marinus str. NATL1A  Bacteria  normal  1  normal  1 
 
 
-
 
NC_007335  PMN2A_0056  HAD family hydrolase  31.79 
 
 		 226 aa  80.9  0.00000000000002  Prochlorococcus marinus str. NATL2A  Bacteria  normal  1  n/a   
 
 
-
 
NC_007434  BURPS1710b_1038  HAD-superfamily hydrolase  34.97 
 
 		 232 aa  80.1  0.00000000000002  Burkholderia pseudomallei 1710b  Bacteria  normal  1  n/a   
 
 
-
 
NC_009076  BURPS1106A_0879  HAD family hydrolase  34.97 
 
 		 232 aa  80.1  0.00000000000002  Burkholderia pseudomallei 1106a  Bacteria  normal  1  n/a   
 
 
-
 
NC_011989  Avi_1034  hydrolase  32.09 
 
 		 217 aa  79.7  0.00000000000003  Agrobacterium vitis S4  Bacteria  normal  1  n/a   
 
 
-
 
NC_008836  BMA10229_A2468  HAD-superfamily hydrolase  34.43 
 
 		 232 aa  79.7  0.00000000000004  Burkholderia mallei NCTC 10229  Bacteria  normal  0.273983  n/a   
 
 
-
 
NC_006348  BMA0336  HAD-superfamily hydrolase  34.43 
 
 		 232 aa  79.7  0.00000000000004  Burkholderia mallei ATCC 23344  Bacteria  normal  0.438572  n/a   
 
 
-
 
NC_009708  YpsIP31758_3236  fructose-1-phosphatase  32.07 
 
 		 188 aa  79.3  0.00000000000004  Yersinia pseudotuberculosis IP 31758  Bacteria  hitchhiker  0.00000000000293158  n/a   
 
 
-
 
NC_010159  YpAngola_A0893  fructose-1-phosphatase  32.07 
 
 		 188 aa  79.3  0.00000000000004  Yersinia pestis Angola  Bacteria  hitchhiker  0.000000160736  normal  1 
 
 
-
 
NC_009080  BMA10247_0082  HAD family hydrolase  34.43 
 
 		 232 aa  79.7  0.00000000000004  Burkholderia mallei NCTC 10247  Bacteria  normal  0.0196618  n/a   
 
 
-
 
NC_010465  YPK_3371  fructose-1-phosphatase  32.07 
 
 		 188 aa  79.3  0.00000000000004  Yersinia pseudotuberculosis YPIII  Bacteria  hitchhiker  0.0000166362  n/a   
 
 
-
 
NC_008785  BMASAVP1_A0634  HAD-superfamily hydrolase  34.43 
 
 		 232 aa  79.7  0.00000000000004  Burkholderia mallei SAVP1  Bacteria  normal  0.535941  n/a   
 
 
-
 
NC_010184  BcerKBAB4_4056  HAD family hydrolase  28 
 
 		 215 aa  79  0.00000000000005  Bacillus weihenstephanensis KBAB4  Bacteria  normal  1  n/a   
 
 
-
 
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