More than 300 homologs were found in PanDaTox collection
for query gene Bfae_25290 on replicon NC_013172
Organism: Brachybacterium faecium DSM 4810



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Replicon accession

Locus tag

Product

% identity

Alignment on query gene

Alignment on homolog gene

Length

Bit score

E-value

Organism name

Kingdom

Plasmid clonability

Plasmid unclonability p-value

Fosmid clonability

Fosmid unclonability p-value

Coverage Plot

PanDaTox homologs

Experimental validation
NC_013172  Bfae_25290  haloacid dehalogenase superfamily enzyme, subfamily IA  100 
 
 
244 aa  478  1e-134  Brachybacterium faecium DSM 4810  Bacteria  normal  0.0218735  n/a   
 
 
-
 
NC_013172  Bfae_25280  haloacid dehalogenase superfamily protein, subfamily IA, variant 3 with third motif having DD or ED  44.39 
 
 
226 aa  134  1.9999999999999998e-30  Brachybacterium faecium DSM 4810  Bacteria  normal  0.0213796  n/a   
 
 
-
 
NC_008541  Arth_1798  phosphatase/phosphohexomutase-like protein  40.28 
 
 
255 aa  125  8.000000000000001e-28  Arthrobacter sp. FB24  Bacteria  hitchhiker  0.00404903  n/a   
 
 
-
 
NC_013131  Caci_2412  HAD-superfamily hydrolase, subfamily IA, variant 3  38.27 
 
 
222 aa  108  8.000000000000001e-23  Catenulispora acidiphila DSM 44928  Bacteria  normal  normal 
 
 
-
 
NC_009654  Mmwyl1_2542  HAD family hydrolase  34.07 
 
 
214 aa  102  8e-21  Marinomonas sp. MWYL1  Bacteria  normal  normal  0.214098 
 
 
-
 
NC_009380  Strop_3972  HAD family hydrolase  35.93 
 
 
241 aa  100  3e-20  Salinispora tropica CNB-440  Bacteria  normal  normal 
 
 
-
 
NC_013093  Amir_2212  HAD-superfamily hydrolase, subfamily IA, variant 3  36.95 
 
 
220 aa  100  3e-20  Actinosynnema mirum DSM 43827  Bacteria  normal  n/a   
 
 
-
 
NC_007777  Francci3_2637  HAD family hydrolase  36.51 
 
 
286 aa  99.8  4e-20  Frankia sp. CcI3  Bacteria  normal  0.403398  normal  0.292856 
 
 
-
 
NC_007005  Psyr_5006  HAD family hydrolase  35.43 
 
 
229 aa  98.6  8e-20  Pseudomonas syringae pv. syringae B728a  Bacteria  normal  normal 
 
 
-
 
NC_009953  Sare_4356  HAD family hydrolase  35.33 
 
 
233 aa  98.6  9e-20  Salinispora arenicola CNS-205  Bacteria  normal  normal  0.582602 
 
 
-
 
NC_009921  Franean1_4889  HAD family hydrolase  39.51 
 
 
233 aa  98.2  1e-19  Frankia sp. EAN1pec  Bacteria  normal  0.351284  normal  0.0730929 
 
 
-
 
NC_008726  Mvan_3473  HAD family hydrolase  36.59 
 
 
243 aa  97.8  1e-19  Mycobacterium vanbaalenii PYR-1  Bacteria  normal  normal 
 
 
-
 
NC_013159  Svir_32070  haloacid dehalogenase superfamily enzyme, subfamily IA  35.6 
 
 
227 aa  96.7  3e-19  Saccharomonospora viridis DSM 43017  Bacteria  normal  0.466176  normal  0.444114 
 
 
-
 
NC_014165  Tbis_1853  HAD-superfamily hydrolase  34.43 
 
 
213 aa  96.3  4e-19  Thermobispora bispora DSM 43833  Bacteria  normal  normal 
 
 
-
 
NC_013093  Amir_7029  HAD-superfamily hydrolase, subfamily IA, variant 3  35.33 
 
 
213 aa  95.5  6e-19  Actinosynnema mirum DSM 43827  Bacteria  normal  n/a   
 
 
-
 
NC_007519  Dde_2523  HAD family hydrolase  34.33 
 
 
219 aa  95.5  7e-19  Desulfovibrio desulfuricans subsp. desulfuricans str. G20  Bacteria  hitchhiker  0.000000000119514  n/a   
 
 
-
 
NC_011353  ECH74115_3936  fructose-1-phosphatase  34.09 
 
 
188 aa  94.4  1e-18  Escherichia coli O157:H7 str. EC4115  Bacteria  hitchhiker  0.00199329  normal  0.845186 
 
 
-
 
NC_009801  EcE24377A_2973  fructose-1-phosphatase  34.09 
 
 
188 aa  94.4  1e-18  Escherichia coli E24377A  Bacteria  hitchhiker  0.000000000945965  n/a   
 
 
-
 
NC_009632  SaurJH1_2387  HAD family hydrolase  33.33 
 
 
211 aa  94.7  1e-18  Staphylococcus aureus subsp. aureus JH1  Bacteria  hitchhiker  0.000185425  n/a   
 
 
-
 
NC_010498  EcSMS35_2812  fructose-1-phosphatase  34.09 
 
 
188 aa  94.4  1e-18  Escherichia coli SMS-3-5  Bacteria  hitchhiker  0.0000062421  hitchhiker  0.000278868 
 
 
-
 
NC_009487  SaurJH9_2344  HAD family hydrolase  33.33 
 
 
211 aa  94.7  1e-18  Staphylococcus aureus subsp. aureus JH9  Bacteria  hitchhiker  0.000000386998  n/a   
 
 
-
 
NC_011080  SNSL254_A3022  fructose-1-phosphatase  34.66 
 
 
188 aa  94  2e-18  Salmonella enterica subsp. enterica serovar Newport str. SL254  Bacteria  normal  0.0218888  decreased coverage  0.00000512461 
 
 
-
 
NC_012917  PC1_3209  fructose-1-phosphatase  32.24 
 
 
188 aa  94  2e-18  Pectobacterium carotovorum subsp. carotovorum PC1  Bacteria  unclonable  0.0000636798  n/a   
 
 
-
 
NC_009656  PSPA7_0665  haloacid dehalogenase, IA family protein  39.25 
 
 
231 aa  94.4  2e-18  Pseudomonas aeruginosa PA7  Bacteria  normal  0.233376  n/a   
 
 
-
 
NC_008463  PA14_07310  putative hydrolase  39.25 
 
 
224 aa  94.4  2e-18  Pseudomonas aeruginosa UCBPP-PA14  Bacteria  normal  0.642028  normal  0.985429 
 
 
-
 
CP001509  ECD_02545  predicted hydrolase  33.52 
 
 
188 aa  93.6  3e-18  Escherichia coli BL21(DE3)  Bacteria  hitchhiker  0.00348702  n/a   
 
 
-
 
NC_009800  EcHS_A2826  fructose-1-phosphatase  33.52 
 
 
188 aa  93.6  3e-18  Escherichia coli HS  Bacteria  hitchhiker  0.000000000139827  n/a   
 
 
-
 
NC_011094  SeSA_A2970  fructose-1-phosphatase  34.66 
 
 
269 aa  93.2  3e-18  Salmonella enterica subsp. enterica serovar Schwarzengrund str. CVM19633  Bacteria  hitchhiker  0.00804181  decreased coverage  0.0000706363 
 
 
-
 
NC_007492  Pfl01_1752  HAD family hydrolase  35.68 
 
 
232 aa  93.6  3e-18  Pseudomonas fluorescens Pf0-1  Bacteria  normal  normal 
 
 
-
 
NC_010468  EcolC_1017  fructose-1-phosphatase  33.52 
 
 
188 aa  93.6  3e-18  Escherichia coli ATCC 8739  Bacteria  normal  0.0370364  hitchhiker  0.0042534 
 
 
-
 
NC_012892  B21_02510  hypothetical protein  33.52 
 
 
188 aa  93.6  3e-18  Escherichia coli BL21  Bacteria  hitchhiker  0.00621233  n/a   
 
 
-
 
NC_011083  SeHA_C3005  fructose-1-phosphatase  34.66 
 
 
188 aa  93.2  4e-18  Salmonella enterica subsp. enterica serovar Heidelberg str. SL476  Bacteria  normal  0.0313059  hitchhiker  0.0000277628 
 
 
-
 
NC_011205  SeD_A3129  fructose-1-phosphatase  34.66 
 
 
188 aa  93.2  4e-18  Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853  Bacteria  hitchhiker  0.000901286  normal  0.0148605 
 
 
-
 
NC_010658  SbBS512_E3186  fructose-1-phosphatase  34.66 
 
 
187 aa  92.4  5e-18  Shigella boydii CDC 3083-94  Bacteria  hitchhiker  0.00000000354509  n/a   
 
 
-
 
NC_011071  Smal_0452  HAD-superfamily hydrolase, subfamily IA, variant 3  37.04 
 
 
227 aa  92  7e-18  Stenotrophomonas maltophilia R551-3  Bacteria  normal  normal 
 
 
-
 
NC_012852  Rleg_6064  HAD-superfamily hydrolase, subfamily IA, variant 3  34.6 
 
 
215 aa  91.3  1e-17  Rhizobium leguminosarum bv. trifolii WSM1325  Bacteria  normal  0.219512  normal 
 
 
-
 
NC_013421  Pecwa_1118  fructose-1-phosphatase  32.07 
 
 
188 aa  90.5  2e-17  Pectobacterium wasabiae WPP163  Bacteria  decreased coverage  0.000766383  n/a   
 
 
-
 
NC_009338  Mflv_3054  HAD family hydrolase  34.15 
 
 
224 aa  90.5  2e-17  Mycobacterium gilvum PYR-GCK  Bacteria  normal  0.751068  normal  0.223571 
 
 
-
 
CP001637  EcDH1_0994  beta-phosphoglucomutase family hydrolase  32.95 
 
 
188 aa  90.1  3e-17  Escherichia coli DH1  Bacteria  hitchhiker  0.000000122872  n/a   
 
 
-
 
NC_011149  SeAg_B2939  fructose-1-phosphatase  34.09 
 
 
188 aa  89.7  3e-17  Salmonella enterica subsp. enterica serovar Agona str. SL483  Bacteria  hitchhiker  0.0000000320797  n/a   
 
 
-
 
NC_004578  PSPTO_5452  HAD-superfamily hydrolase  36.54 
 
 
212 aa  89.7  4e-17  Pseudomonas syringae pv. tomato str. DC3000  Bacteria  normal  n/a   
 
 
-
 
NC_013159  Svir_39300  haloacid dehalogenase superfamily protein, subfamily IA, variant 3 with third motif having DD or ED  35.33 
 
 
218 aa  89  6e-17  Saccharomonospora viridis DSM 43017  Bacteria  normal  normal 
 
 
-
 
NC_013595  Sros_7385  HAD-superfamily hydrolase, subfamily IA  37.62 
 
 
248 aa  88.6  7e-17  Streptosporangium roseum DSM 43021  Bacteria  normal  normal 
 
 
-
 
NC_007947  Mfla_1543  HAD family hydrolase  34.81 
 
 
218 aa  88.6  8e-17  Methylobacillus flagellatus KT  Bacteria  normal  normal  0.290377 
 
 
-
 
NC_007413  Ava_3074  HAD family hydrolase  33.85 
 
 
222 aa  88.2  1e-16  Anabaena variabilis ATCC 29413  Bacteria  normal  normal 
 
 
-
 
NC_013595  Sros_5916  HAD family hydrolase  33.33 
 
 
218 aa  88.2  1e-16  Streptosporangium roseum DSM 43021  Bacteria  normal  0.0345843  normal  0.0351614 
 
 
-
 
NC_009832  Spro_0845  fructose-1-phosphatase  30.6 
 
 
188 aa  88.2  1e-16  Serratia proteamaculans 568  Bacteria  hitchhiker  0.0000000012907  hitchhiker  0.000000242912 
 
 
-
 
NC_008639  Cpha266_2591  HAD family hydrolase  36.41 
 
 
220 aa  87.4  2e-16  Chlorobium phaeobacteroides DSM 266  Bacteria  hitchhiker  0.0000123522  n/a   
 
 
-
 
NC_011989  Avi_1034  hydrolase  35.64 
 
 
217 aa  87  3e-16  Agrobacterium vitis S4  Bacteria  normal  n/a   
 
 
-
 
NC_009719  Plav_2303  HAD family hydrolase  36.17 
 
 
230 aa  86.7  3e-16  Parvibaculum lavamentivorans DS-1  Bacteria  normal  0.432343  normal  0.0684019 
 
 
-
 
NC_007493  RSP_0089  hypothetical protein  38.12 
 
 
228 aa  86.7  3e-16  Rhodobacter sphaeroides 2.4.1  Bacteria  normal  0.16166  n/a   
 
 
-
 
NC_009441  Fjoh_2690  HAD family hydrolase  30.6 
 
 
221 aa  86.3  4e-16  Flavobacterium johnsoniae UW101  Bacteria  normal  0.365385  n/a   
 
 
-
 
NC_013730  Slin_2846  HAD-superfamily hydrolase, subfamily IA, variant 3  32.28 
 
 
226 aa  85.9  6e-16  Spirosoma linguale DSM 74  Bacteria  normal  0.281035  normal  0.462582 
 
 
-
 
NC_012793  GWCH70_1743  HAD-superfamily hydrolase, subfamily IA, variant 3  29.89 
 
 
227 aa  85.5  8e-16  Geobacillus sp. WCH70  Bacteria  normal  0.293469  n/a   
 
 
-
 
NC_014210  Ndas_1550  HAD-superfamily hydrolase, subfamily IA, variant 3  32.61 
 
 
249 aa  85.5  8e-16  Nocardiopsis dassonvillei subsp. dassonvillei DSM 43111  Bacteria  normal  normal  0.204001 
 
 
-
 
NC_002977  MCA2733  HAD-superfamily hydrolase, subfamily IA, variant 3 family protein  36.1 
 
 
237 aa  84.7  0.000000000000001  Methylococcus capsulatus str. Bath  Bacteria  normal  0.53567  n/a   
 
 
-
 
NC_007954  Sden_3378  2-deoxyglucose-6-phosphatase  30.93 
 
 
225 aa  84.7  0.000000000000001  Shewanella denitrificans OS217  Bacteria  normal  n/a   
 
 
-
 
NC_013510  Tcur_2293  HAD-superfamily hydrolase, subfamily IA, variant 3  34.46 
 
 
218 aa  84.7  0.000000000000001  Thermomonospora curvata DSM 43183  Bacteria  hitchhiker  0.0000494788  n/a   
 
 
-
 
NC_009092  Shew_3425  2-deoxyglucose-6-phosphatase  31.77 
 
 
223 aa  84  0.000000000000002  Shewanella loihica PV-4  Bacteria  hitchhiker  0.000919178  normal 
 
 
-
 
NC_004116  SAG0181  HAD superfamily hydrolase  28.99 
 
 
214 aa  83.6  0.000000000000002  Streptococcus agalactiae 2603V/R  Bacteria  normal  n/a   
 
 
-
 
NC_007643  Rru_A1268  HAD family hydrolase  36.84 
 
 
215 aa  83.6  0.000000000000002  Rhodospirillum rubrum ATCC 11170  Bacteria  normal  n/a   
 
 
-
 
NC_013037  Dfer_2943  HAD-superfamily hydrolase, subfamily IA, variant 3  30.3 
 
 
218 aa  83.2  0.000000000000003  Dyadobacter fermentans DSM 18053  Bacteria  normal  0.86932  normal 
 
 
-
 
NC_013595  Sros_1428  putative phosphatase  33.74 
 
 
212 aa  83.6  0.000000000000003  Streptosporangium roseum DSM 43021  Bacteria  normal  0.307964  normal  0.0744833 
 
 
-
 
NC_008700  Sama_0381  2-deoxyglucose-6-phosphatase  31.96 
 
 
221 aa  83.6  0.000000000000003  Shewanella amazonensis SB2B  Bacteria  hitchhiker  0.0071153  normal  0.324571 
 
 
-
 
NC_013173  Dbac_1634  HAD-superfamily hydrolase, subfamily IA, variant 3  32.29 
 
 
221 aa  83.2  0.000000000000004  Desulfomicrobium baculatum DSM 4028  Bacteria  normal  0.0501975  n/a   
 
 
-
 
NC_012912  Dd1591_0985  fructose-1-phosphatase  31.35 
 
 
188 aa  82.8  0.000000000000004  Dickeya zeae Ech1591  Bacteria  decreased coverage  0.000117667  n/a   
 
 
-
 
NC_009436  Ent638_3170  fructose-1-phosphatase  32.2 
 
 
188 aa  82.8  0.000000000000004  Enterobacter sp. 638  Bacteria  hitchhiker  0.00217793  normal 
 
 
-
 
NC_010001  Cphy_2790  HAD family hydrolase  31.34 
 
 
223 aa  82.8  0.000000000000005  Clostridium phytofermentans ISDg  Bacteria  normal  n/a   
 
 
-
 
NC_008009  Acid345_3787  HAD family hydrolase  30.27 
 
 
238 aa  82.4  0.000000000000005  Candidatus Koribacter versatilis Ellin345  Bacteria  normal  0.85061  normal 
 
 
-
 
NC_009428  Rsph17025_1679  HAD family hydrolase  36.31 
 
 
228 aa  82.8  0.000000000000005  Rhodobacter sphaeroides ATCC 17025  Bacteria  normal  0.329871  normal 
 
 
-
 
NC_013730  Slin_0267  HAD-superfamily hydrolase, subfamily IA, variant 3  30.77 
 
 
220 aa  82.8  0.000000000000005  Spirosoma linguale DSM 74  Bacteria  normal  0.583067  normal  0.103324 
 
 
-
 
NC_009438  Sputcn32_3410  2-deoxyglucose-6-phosphatase  31.35 
 
 
217 aa  82  0.000000000000007  Shewanella putrefaciens CN-32  Bacteria  normal  0.0110715  n/a   
 
 
-
 
NC_010571  Oter_1593  beta-phosphoglucomutase family hydrolase  34.76 
 
 
202 aa  82  0.000000000000007  Opitutus terrae PB90-1  Bacteria  normal  normal  0.113259 
 
 
-
 
NC_013205  Aaci_2469  HAD-superfamily hydrolase, subfamily IA, variant 3  34.41 
 
 
224 aa  82  0.000000000000008  Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446  Bacteria  normal  0.618161  n/a   
 
 
-
 
NC_008148  Rxyl_0905  HAD family hydrolase  33.52 
 
 
204 aa  82  0.000000000000008  Rubrobacter xylanophilus DSM 9941  Bacteria  normal  0.427836  n/a   
 
 
-
 
NC_009972  Haur_4152  HAD family hydrolase  33.33 
 
 
217 aa  82  0.000000000000008  Herpetosiphon aurantiacus ATCC 23779  Bacteria  normal  n/a   
 
 
-
 
NC_009708  YpsIP31758_3236  fructose-1-phosphatase  29.44 
 
 
188 aa  81.6  0.000000000000009  Yersinia pseudotuberculosis IP 31758  Bacteria  hitchhiker  0.00000000000293158  n/a   
 
 
-
 
NC_010465  YPK_3371  fructose-1-phosphatase  29.44 
 
 
188 aa  81.6  0.000000000000009  Yersinia pseudotuberculosis YPIII  Bacteria  hitchhiker  0.0000166362  n/a   
 
 
-
 
NC_010159  YpAngola_A0893  fructose-1-phosphatase  29.44 
 
 
188 aa  81.6  0.000000000000009  Yersinia pestis Angola  Bacteria  hitchhiker  0.000000160736  normal 
 
 
-
 
NC_013037  Dfer_4825  HAD-superfamily hydrolase, subfamily IA, variant 3  31.52 
 
 
224 aa  81.3  0.00000000000001  Dyadobacter fermentans DSM 18053  Bacteria  normal  normal 
 
 
-
 
NC_013526  Tter_2485  HAD-superfamily hydrolase, subfamily IA, variant 3  33.7 
 
 
238 aa  81.6  0.00000000000001  Thermobaculum terrenum ATCC BAA-798  Bacteria  normal  n/a   
 
 
-
 
NC_011365  Gdia_2538  HAD-superfamily hydrolase, subfamily IA, variant 3  31.9 
 
 
241 aa  81.6  0.00000000000001  Gluconacetobacter diazotrophicus PAl 5  Bacteria  normal  normal 
 
 
-
 
NC_010717  PXO_03084  HAD superfamily hydrolase  34.03 
 
 
226 aa  81.6  0.00000000000001  Xanthomonas oryzae pv. oryzae PXO99A  Bacteria  normal  n/a   
 
 
-
 
NC_008312  Tery_0680  HAD family hydrolase  31.18 
 
 
227 aa  81.3  0.00000000000001  Trichodesmium erythraeum IMS101  Bacteria  normal  normal  0.0123656 
 
 
-
 
NC_007604  Synpcc7942_1351  HAD family hydrolase  33.8 
 
 
236 aa  80.5  0.00000000000002  Synechococcus elongatus PCC 7942  Bacteria  decreased coverage  0.00941248  normal 
 
 
-
 
NC_013522  Taci_1058  HAD-superfamily hydrolase, subfamily IA, variant 3  36.61 
 
 
217 aa  80.9  0.00000000000002  Thermanaerovibrio acidaminovorans DSM 6589  Bacteria  normal  0.918987  n/a   
 
 
-
 
NC_011831  Cagg_1516  HAD-superfamily hydrolase, subfamily IA, variant 3  36.1 
 
 
227 aa  80.9  0.00000000000002  Chloroflexus aggregans DSM 9485  Bacteria  normal  normal 
 
 
-
 
NC_003909  BCE_4276  HAD superfamily hydrolase  31.9 
 
 
220 aa  80.1  0.00000000000003  Bacillus cereus ATCC 10987  Bacteria  normal  n/a   
 
 
-
 
NC_009012  Cthe_3073  HAD family hydrolase  28.49 
 
 
223 aa  80.1  0.00000000000003  Clostridium thermocellum ATCC 27405  Bacteria  hitchhiker  0.00582956  n/a   
 
 
-
 
NC_013061  Phep_1395  HAD-superfamily hydrolase, subfamily IA, variant 3  31.18 
 
 
201 aa  80.1  0.00000000000003  Pedobacter heparinus DSM 2366  Bacteria  normal  normal  0.0347939 
 
 
-
 
NC_006348  BMA0717  HAD-superfamily hydrolase  35.75 
 
 
224 aa  79.7  0.00000000000004  Burkholderia mallei ATCC 23344  Bacteria  normal  n/a   
 
 
-
 
NC_008836  BMA10229_A2990  HAD-superfamily hydrolase  35.75 
 
 
224 aa  79.7  0.00000000000004  Burkholderia mallei NCTC 10229  Bacteria  normal  n/a   
 
 
-
 
NC_009080  BMA10247_1609  HAD-superfamily hydrolase  35.75 
 
 
224 aa  79.7  0.00000000000004  Burkholderia mallei NCTC 10247  Bacteria  normal  n/a   
 
 
-
 
NC_009074  BURPS668_1060  haloacid dehalogenase, IA family protein  35.75 
 
 
224 aa  79.7  0.00000000000004  Burkholderia pseudomallei 668  Bacteria  normal  n/a   
 
 
-
 
NC_008785  BMASAVP1_A2296  HAD-superfamily hydrolase  35.75 
 
 
224 aa  79.7  0.00000000000004  Burkholderia mallei SAVP1  Bacteria  normal  n/a   
 
 
-
 
NC_009076  BURPS1106A_1066  haloacid dehalogenase, IA family protein  35.42 
 
 
224 aa  79.3  0.00000000000005  Burkholderia pseudomallei 1106a  Bacteria  normal  n/a   
 
 
-
 
NC_013235  Namu_2787  HAD-superfamily hydrolase, subfamily IA, variant 3  35.68 
 
 
230 aa  79  0.00000000000006  Nakamurella multipartita DSM 44233  Bacteria  hitchhiker  0.00137861  hitchhiker  0.0000132599 
 
 
-
 
NC_008578  Acel_1175  HAD family hydrolase  35.08 
 
 
263 aa  78.6  0.00000000000008  Acidothermus cellulolyticus 11B  Bacteria  normal  normal 
 
 
-
 
NC_007413  Ava_0109  HAD family hydrolase  32.79 
 
 
223 aa  78.2  0.0000000000001  Anabaena variabilis ATCC 29413  Bacteria  normal  decreased coverage  0.00453013 
 
 
-
 
NC_007434  BURPS1710b_0896  HAD-superfamily hydrolase  33.33 
 
 
228 aa  77.8  0.0000000000001  Burkholderia pseudomallei 1710b  Bacteria  normal  n/a   
 
 
-
 
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