218 homologs were found in PanDaTox collection
for query gene Xaut_0899 on replicon NC_009720
Organism: Xanthobacter autotrophicus Py2



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Replicon accession

Locus tag

Product

% identity

Alignment on query gene

Alignment on homolog gene

Length

Bit score

E-value

Organism name

Kingdom

Plasmid clonability

Plasmid unclonability p-value

Fosmid clonability

Fosmid unclonability p-value

Coverage Plot

PanDaTox homologs

Experimental validation
NC_009720  Xaut_0899  phenylacetate-CoA oxygenase, PaaJ subunit  100 
 
 
168 aa  340  5e-93  Xanthobacter autotrophicus Py2  Bacteria  normal  normal 
 
 
-
 
NC_009485  BBta_2868  phenylacetic acid degradation protein paaD  69.38 
 
 
167 aa  239  9e-63  Bradyrhizobium sp. BTAi1  Bacteria  normal  0.499759  normal  0.217404 
 
 
-
 
NC_011004  Rpal_4286  phenylacetate-CoA oxygenase, PaaJ subunit  70.44 
 
 
167 aa  239  2e-62  Rhodopseudomonas palustris TIE-1  Bacteria  normal  0.335667  n/a   
 
 
-
 
NC_007778  RPB_3638  phenylacetate-CoA oxygenase, PaaJ subunit  66.67 
 
 
167 aa  238  4e-62  Rhodopseudomonas palustris HaA2  Bacteria  normal  normal  0.315458 
 
 
-
 
NC_007958  RPD_1827  phenylacetate-CoA oxygenase, PaaJ subunit  67.92 
 
 
166 aa  234  3e-61  Rhodopseudomonas palustris BisB5  Bacteria  normal  0.0959746  normal 
 
 
-
 
NC_007925  RPC_0688  phenylacetate-CoA oxygenase, PaaJ subunit  63.64 
 
 
167 aa  223  8e-58  Rhodopseudomonas palustris BisB18  Bacteria  normal  normal 
 
 
-
 
NC_011894  Mnod_7196  phenylacetate-CoA oxygenase, PaaJ subunit  63.01 
 
 
160 aa  195  3e-49  Methylobacterium nodulans ORS 2060  Bacteria  normal  0.689792  n/a   
 
 
-
 
NC_009484  Acry_2611  phenylacetate-CoA oxygenase, PaaJ subunit  62.91 
 
 
163 aa  191  6e-48  Acidiphilium cryptum JF-5  Bacteria  normal  n/a   
 
 
-
 
NC_009654  Mmwyl1_3092  phenylacetate-CoA oxygenase, PaaJ subunit  55.26 
 
 
177 aa  181  3e-45  Marinomonas sp. MWYL1  Bacteria  normal  0.278581  hitchhiker  0.000748353 
 
 
-
 
NC_009511  Swit_0756  phenylacetate-CoA oxygenase, PaaJ subunit  53.01 
 
 
166 aa  177  4.999999999999999e-44  Sphingomonas wittichii RW1  Bacteria  normal  0.174345  normal  0.16936 
 
 
-
 
NC_007951  Bxe_A0292  phenylacetic acid degradation protein PaaD  56.71 
 
 
186 aa  177  7e-44  Burkholderia xenovorans LB400  Bacteria  normal  normal 
 
 
-
 
NC_010622  Bphy_2860  phenylacetate-CoA oxygenase, PaaJ subunit  55.42 
 
 
191 aa  176  2e-43  Burkholderia phymatum STM815  Bacteria  normal  hitchhiker  0.00211249 
 
 
-
 
NC_011886  Achl_2961  phenylacetate-CoA oxygenase, PaaJ subunit  55.06 
 
 
176 aa  176  2e-43  Arthrobacter chlorophenolicus A6  Bacteria  n/a    normal 
 
 
-
 
NC_010681  Bphyt_3665  phenylacetate-CoA oxygenase, PaaJ subunit  56.52 
 
 
183 aa  175  3e-43  Burkholderia phytofirmans PsJN  Bacteria  normal  0.0979588  hitchhiker  0.000000287258 
 
 
-
 
NC_010084  Bmul_0229  phenylacetate-CoA oxygenase, PaaJ subunit  55.17 
 
 
187 aa  175  3e-43  Burkholderia multivorans ATCC 17616  Bacteria  hitchhiker  0.000381219  hitchhiker  0.00114701 
 
 
-
 
NC_008541  Arth_3215  phenylacetate-CoA oxygenase, PaaJ subunit  53.49 
 
 
189 aa  174  5e-43  Arthrobacter sp. FB24  Bacteria  normal  0.667172  n/a   
 
 
-
 
NC_003296  RSp0607  phenylacetic acid degradation protein  57.52 
 
 
182 aa  174  6e-43  Ralstonia solanacearum GMI1000  Bacteria  normal  0.520929  normal 
 
 
-
 
NC_012803  Mlut_20180  phenylacetate-CoA oxygenase, PaaJ subunit  55.21 
 
 
169 aa  173  8e-43  Micrococcus luteus NCTC 2665  Bacteria  normal  0.77293  n/a   
 
 
-
 
NC_014230  CA2559_05315  putative phenylacetic acid degradation protein  53.46 
 
 
168 aa  172  1.9999999999999998e-42  Croceibacter atlanticus HTCC2559  Bacteria  normal  0.633098  n/a   
 
 
-
 
NC_009074  BURPS668_3778  phenylacetate-CoA oxygenase, PaaJ subunit  53.8 
 
 
206 aa  171  3.9999999999999995e-42  Burkholderia pseudomallei 668  Bacteria  hitchhiker  0.0078735  n/a   
 
 
-
 
NC_007510  Bcep18194_A3426  phenylacetate-CoA oxygenase, PaaJ subunit  52.84 
 
 
187 aa  170  9e-42  Burkholderia sp. 383  Bacteria  normal  0.0224942  normal  0.802561 
 
 
-
 
NC_010508  Bcenmc03_0307  phenylacetate-CoA oxygenase, PaaJ subunit  55.62 
 
 
187 aa  169  1e-41  Burkholderia cenocepacia MC0-3  Bacteria  normal  0.0405135  hitchhiker  0.00121913 
 
 
-
 
NC_008060  Bcen_2779  phenylacetate-CoA oxygenase, PaaJ subunit  54.55 
 
 
187 aa  170  1e-41  Burkholderia cenocepacia AU 1054  Bacteria  normal  0.241075  n/a   
 
 
-
 
NC_008542  Bcen2424_0327  phenylacetate-CoA oxygenase, PaaJ subunit  54.55 
 
 
187 aa  170  1e-41  Burkholderia cenocepacia HI2424  Bacteria  normal  0.225385  n/a   
 
 
-
 
NC_009076  BURPS1106A_3839  phenylacetate-CoA oxygenase, PaaJ subunit  54.44 
 
 
206 aa  169  2e-41  Burkholderia pseudomallei 1106a  Bacteria  normal  0.61157  n/a   
 
 
-
 
NC_007434  BURPS1710b_0010  phenylacetic acid degradation protein PaaD  53.85 
 
 
206 aa  168  3e-41  Burkholderia pseudomallei 1710b  Bacteria  normal  n/a   
 
 
-
 
NC_008390  Bamb_0246  phenylacetate-CoA oxygenase, PaaJ subunit  55.62 
 
 
187 aa  168  3e-41  Burkholderia ambifaria AMMD  Bacteria  normal  0.415346  n/a   
 
 
-
 
NC_010322  PputGB1_2621  phenylacetate-CoA oxygenase, PaaJ subunit  51.32 
 
 
177 aa  168  3e-41  Pseudomonas putida GB-1  Bacteria  normal  normal  0.324039 
 
 
-
 
NC_009512  Pput_2484  phenylacetate-CoA oxygenase, PaaJ subunit  51.97 
 
 
177 aa  168  3e-41  Pseudomonas putida F1  Bacteria  normal  0.323944  normal 
 
 
-
 
NC_002947  PP_3275  phenylacetate-CoA oxygenase, PaaJ subunit  51.32 
 
 
177 aa  168  4e-41  Pseudomonas putida KT2440  Bacteria  normal  0.721479  normal 
 
 
-
 
NC_010551  BamMC406_0255  phenylacetate-CoA oxygenase, PaaJ subunit  55.62 
 
 
187 aa  168  4e-41  Burkholderia ambifaria MC40-6  Bacteria  hitchhiker  0.00867591  normal  0.026194 
 
 
-
 
NC_007347  Reut_A3209  phenylacetate-CoA oxygenase, PaaJ subunit  51.4 
 
 
180 aa  167  5e-41  Ralstonia eutropha JMP134  Bacteria  normal  n/a   
 
 
-
 
NC_008699  Noca_3156  phenylacetate-CoA oxygenase, PaaJ subunit  55.97 
 
 
161 aa  167  6e-41  Nocardioides sp. JS614  Bacteria  normal  n/a   
 
 
-
 
NC_006348  BMA2576  phenylacetic acid degradation protein PaaD  53.25 
 
 
206 aa  167  8e-41  Burkholderia mallei ATCC 23344  Bacteria  normal  0.102091  n/a   
 
 
-
 
NC_008785  BMASAVP1_A0009  phenylacetate-CoA oxygenase, PaaJ subunit  53.25 
 
 
206 aa  167  8e-41  Burkholderia mallei SAVP1  Bacteria  hitchhiker  0.000409073  n/a   
 
 
-
 
NC_008785  BMASAVP1_A3114  phenylacetic acid degradation protein PaaD  53.25 
 
 
206 aa  167  8e-41  Burkholderia mallei SAVP1  Bacteria  hitchhiker  0.000394785  n/a   
 
 
-
 
NC_007651  BTH_I3100  phenylacetic acid degradation protein PaaD  52.07 
 
 
289 aa  166  2e-40  Burkholderia thailandensis E264  Bacteria  normal  0.0713561  n/a   
 
 
-
 
NC_012857  Rpic12D_3492  phenylacetate-CoA oxygenase, PaaJ subunit  54.14 
 
 
183 aa  165  2.9999999999999998e-40  Ralstonia pickettii 12D  Bacteria  normal  normal  0.318396 
 
 
-
 
NC_013235  Namu_2488  phenylacetate-CoA oxygenase, PaaJ subunit  52.66 
 
 
180 aa  165  2.9999999999999998e-40  Nakamurella multipartita DSM 44233  Bacteria  hitchhiker  0.0000115615  normal  0.034342 
 
 
-
 
NC_010678  Rpic_4564  phenylacetate-CoA oxygenase, PaaJ subunit  54.14 
 
 
183 aa  165  2.9999999999999998e-40  Ralstonia pickettii 12J  Bacteria  normal  normal  0.583831 
 
 
-
 
NC_008043  TM1040_3727  phenylacetate-CoA oxygenase, PaaJ subunit  47.31 
 
 
158 aa  164  5e-40  Ruegeria sp. TM1040  Bacteria  normal  normal  0.784868 
 
 
-
 
NC_007298  Daro_0380  phenylacetate-CoA oxygenase, PaaJ subunit  52.87 
 
 
166 aa  163  9e-40  Dechloromonas aromatica RCB  Bacteria  normal  0.141458  hitchhiker  0.00815005 
 
 
-
 
NC_011662  Tmz1t_1503  phenylacetate-CoA oxygenase, PaaJ subunit  48.81 
 
 
170 aa  163  1.0000000000000001e-39  Thauera sp. MZ1T  Bacteria  normal  0.539813  n/a   
 
 
-
 
NC_010501  PputW619_2634  phenylacetate-CoA oxygenase, PaaJ subunit  49.34 
 
 
177 aa  163  1.0000000000000001e-39  Pseudomonas putida W619  Bacteria  normal  0.616959  normal  0.117907 
 
 
-
 
NC_013739  Cwoe_2201  phenylacetate-CoA oxygenase, PaaJ subunit  54.78 
 
 
155 aa  158  3e-38  Conexibacter woesei DSM 14684  Bacteria  normal  0.124529  normal 
 
 
-
 
NC_009832  Spro_3075  phenylacetate-CoA oxygenase, PaaJ subunit  49.35 
 
 
165 aa  157  4e-38  Serratia proteamaculans 568  Bacteria  normal  normal  0.14489 
 
 
-
 
NC_007348  Reut_B3738  phenylacetate-CoA oxygenase, PaaJ subunit  49.69 
 
 
171 aa  157  5e-38  Ralstonia eutropha JMP134  Bacteria  normal  0.188896  n/a   
 
 
-
 
NC_007973  Rmet_3372  phenylacetate-CoA oxygenase PaaJ  52.83 
 
 
202 aa  157  8e-38  Cupriavidus metallidurans CH34  Bacteria  normal  normal 
 
 
-
 
NC_009956  Dshi_3821  phenylacetate-CoA oxygenase, PaaJ subunit  50.33 
 
 
152 aa  157  9e-38  Dinoroseobacter shibae DFL 12  Bacteria  normal  0.134466  normal  0.475873 
 
 
-
 
NC_010524  Lcho_3571  phenylacetate-CoA oxygenase, PaaJ subunit  47.46 
 
 
183 aa  156  1e-37  Leptothrix cholodnii SP-6  Bacteria  n/a    normal  0.139569 
 
 
-
 
NC_008825  Mpe_A0990  putative ring oxidation complex protein 3  47.9 
 
 
182 aa  155  2e-37  Methylibium petroleiphilum PM1  Bacteria  normal  0.190413  normal 
 
 
-
 
NC_008688  Pden_4807  phenylacetate-CoA oxygenase, PaaJ subunit  49.08 
 
 
173 aa  155  3e-37  Paracoccus denitrificans PD1222  Bacteria  normal  normal  0.553285 
 
 
-
 
NC_013757  Gobs_1904  phenylacetate-CoA oxygenase, PaaJ subunit  54.49 
 
 
174 aa  154  5.0000000000000005e-37  Geodermatophilus obscurus DSM 43160  Bacteria  normal  n/a   
 
 
-
 
NC_007964  Nham_0931  phenylacetate-CoA oxygenase, PaaJ subunit  44.64 
 
 
174 aa  154  6e-37  Nitrobacter hamburgensis X14  Bacteria  normal  n/a   
 
 
-
 
NC_013093  Amir_6689  phenylacetate-CoA oxygenase, PaaJ subunit  49.43 
 
 
170 aa  152  2e-36  Actinosynnema mirum DSM 43827  Bacteria  normal  0.342099  n/a   
 
 
-
 
NC_007802  Jann_0652  phenylacetate-CoA oxygenase, PaaJ subunit  46.75 
 
 
157 aa  152  2e-36  Jannaschia sp. CCS1  Bacteria  normal  normal 
 
 
-
 
NC_008709  Ping_0653  phenylacetate-CoA oxygenase, PaaJ subunit  49.02 
 
 
168 aa  152  2.9999999999999998e-36  Psychromonas ingrahamii 37  Bacteria  normal  normal 
 
 
-
 
NC_011894  Mnod_3353  phenylacetate-CoA oxygenase, PaaJ subunit  49.39 
 
 
174 aa  151  4e-36  Methylobacterium nodulans ORS 2060  Bacteria  normal  n/a   
 
 
-
 
NC_013159  Svir_26500  phenylacetate-CoA oxygenase, PaaJ subunit  48.73 
 
 
167 aa  149  1e-35  Saccharomonospora viridis DSM 43017  Bacteria  normal  0.4071  normal  0.146126 
 
 
-
 
NC_012791  Vapar_1232  phenylacetate-CoA oxygenase, PaaJ subunit  46.3 
 
 
176 aa  147  5e-35  Variovorax paradoxus S110  Bacteria  normal  n/a   
 
 
-
 
NC_009801  EcE24377A_1576  phenylacetate-CoA oxygenase, PaaJ subunit  46.67 
 
 
165 aa  147  6e-35  Escherichia coli E24377A  Bacteria  normal  n/a   
 
 
-
 
NC_009800  EcHS_A1478  phenylacetate-CoA oxygenase, PaaJ subunit  46.67 
 
 
165 aa  147  6e-35  Escherichia coli HS  Bacteria  normal  n/a   
 
 
-
 
NC_009620  Smed_4150  phenylacetate-CoA oxygenase, PaaJ subunit  47.56 
 
 
174 aa  147  6e-35  Sinorhizobium medicae WSM419  Bacteria  normal  normal  0.963466 
 
 
-
 
CP001637  EcDH1_2254  phenylacetate-CoA oxygenase, PaaJ subunit  46.67 
 
 
165 aa  147  7e-35  Escherichia coli DH1  Bacteria  normal  n/a   
 
 
-
 
NC_010002  Daci_0813  phenylacetate-CoA oxygenase, PaaJ subunit  42.78 
 
 
189 aa  145  3e-34  Delftia acidovorans SPH-1  Bacteria  normal  0.165095  normal 
 
 
-
 
NC_008025  Dgeo_1915  phenylacetate-CoA oxygenase, PaaJ subunit  47.74 
 
 
171 aa  144  5e-34  Deinococcus geothermalis DSM 11300  Bacteria  normal  0.844425  normal  0.11814 
 
 
-
 
NC_013946  Mrub_2413  phenylacetate-CoA oxygenase PaaJ  41.29 
 
 
164 aa  144  6e-34  Meiothermus ruber DSM 1279  Bacteria  normal  normal 
 
 
-
 
NC_010468  EcolC_2264  phenylacetate-CoA oxygenase, PaaJ subunit  46.06 
 
 
165 aa  144  7.0000000000000006e-34  Escherichia coli ATCC 8739  Bacteria  normal  0.91421  normal 
 
 
-
 
NC_008786  Veis_3948  phenylacetate-CoA oxygenase, PaaJ subunit  45.12 
 
 
169 aa  144  7.0000000000000006e-34  Verminephrobacter eiseniae EF01-2  Bacteria  normal  0.641663  normal  0.597534 
 
 
-
 
NC_013169  Ksed_09320  phenylacetate-CoA oxygenase, PaaJ subunit  49.38 
 
 
174 aa  142  2e-33  Kytococcus sedentarius DSM 20547  Bacteria  normal  normal 
 
 
-
 
NC_009953  Sare_4469  phenylacetate-CoA oxygenase, PaaJ subunit  47.97 
 
 
158 aa  140  8e-33  Salinispora arenicola CNS-205  Bacteria  normal  0.475576  normal 
 
 
-
 
NC_014210  Ndas_2478  phenylacetate-CoA oxygenase, PaaJ subunit  47.37 
 
 
173 aa  140  9e-33  Nocardiopsis dassonvillei subsp. dassonvillei DSM 43111  Bacteria  normal  normal 
 
 
-
 
NC_014212  Mesil_3042  phenylacetate-CoA oxygenase, PaaJ subunit  42.21 
 
 
164 aa  137  4.999999999999999e-32  Meiothermus silvanus DSM 9946  Bacteria  normal  normal 
 
 
-
 
NC_013501  Rmar_1546  phenylacetate-CoA oxygenase, PaaJ subunit  46.88 
 
 
166 aa  135  2e-31  Rhodothermus marinus DSM 4252  Bacteria  normal  n/a   
 
 
-
 
NC_008578  Acel_1440  phenylacetate-CoA oxygenase, PaaJ subunit  48.1 
 
 
149 aa  135  3.0000000000000003e-31  Acidothermus cellulolyticus 11B  Bacteria  normal  normal 
 
 
-
 
NC_013411  GYMC61_2866  phenylacetate-CoA oxygenase, PaaJ subunit  42.21 
 
 
157 aa  130  1.0000000000000001e-29  Geobacillus sp. Y412MC61  Bacteria  n/a    n/a   
 
 
-
 
NC_013947  Snas_1241  phenylacetate-CoA oxygenase PaaJ subunit  41.56 
 
 
164 aa  127  9.000000000000001e-29  Stackebrandtia nassauensis DSM 44728  Bacteria  normal  normal 
 
 
-
 
NC_010506  Swoo_0218  phenylacetate-CoA oxygenase, PaaJ subunit  42.11 
 
 
175 aa  125  2.0000000000000002e-28  Shewanella woodyi ATCC 51908  Bacteria  normal  normal  0.0145597 
 
 
-
 
NC_009380  Strop_4070  phenylacetate-CoA oxygenase, PaaJ subunit  49.32 
 
 
158 aa  122  2e-27  Salinispora tropica CNB-440  Bacteria  normal  normal  0.177293 
 
 
-
 
NC_013132  Cpin_0790  phenylacetate-CoA oxygenase, PaaJ subunit  39.07 
 
 
163 aa  115  3e-25  Chitinophaga pinensis DSM 2588  Bacteria  normal  normal  0.12987 
 
 
-
 
NC_013205  Aaci_0810  phenylacetate-CoA oxygenase, PaaJ subunit  37.25 
 
 
154 aa  102  3e-21  Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446  Bacteria  normal  n/a   
 
 
-
 
NC_013131  Caci_1747  protein of unknown function DUF59  47.17 
 
 
325 aa  89.4  2e-17  Catenulispora acidiphila DSM 44928  Bacteria  normal  0.335783  normal 
 
 
-
 
NC_008836  BMA10229_A1981  phenylacetic acid degradation protein PaaD  49.46 
 
 
95 aa  86.7  1e-16  Burkholderia mallei NCTC 10229  Bacteria  normal  0.196807  n/a   
 
 
-
 
NC_013922  Nmag_1024  protein of unknown function DUF59  36.79 
 
 
142 aa  76.6  0.0000000000001  Natrialba magadii ATCC 43099  Archaea  normal  0.929199  n/a   
 
 
-
 
NC_014212  Mesil_2422  protein of unknown function DUF59  39.39 
 
 
112 aa  72.4  0.000000000003  Meiothermus silvanus DSM 9946  Bacteria  normal  normal 
 
 
-
 
NC_013946  Mrub_0141  hypothetical protein  37 
 
 
124 aa  67.4  0.00000000008  Meiothermus ruber DSM 1279  Bacteria  hitchhiker  0.00350528  normal 
 
 
-
 
NC_013743  Htur_2885  protein of unknown function DUF59  35.24 
 
 
138 aa  65.5  0.0000000003  Haloterrigena turkmenica DSM 5511  Archaea  n/a    n/a   
 
 
-
 
NC_008025  Dgeo_1281  hypothetical protein  36.73 
 
 
118 aa  63.9  0.000000001  Deinococcus geothermalis DSM 11300  Bacteria  normal  normal  0.654013 
 
 
-
 
NC_013926  Aboo_0118  protein of unknown function DUF59  37.76 
 
 
99 aa  60.5  0.00000001  Aciduliprofundum boonei T469  Archaea  normal  n/a   
 
 
-
 
NC_013411  GYMC61_1551  protein of unknown function DUF59  35.71 
 
 
100 aa  58.5  0.00000004  Geobacillus sp. Y412MC61  Bacteria  n/a    n/a   
 
 
-
 
NC_013744  Htur_4173  protein of unknown function DUF59  32.08 
 
 
131 aa  58.2  0.00000005  Haloterrigena turkmenica DSM 5511  Archaea  normal  0.240883  n/a   
 
 
-
 
NC_012793  GWCH70_0721  protein of unknown function DUF59  34.69 
 
 
100 aa  58.2  0.00000006  Geobacillus sp. WCH70  Bacteria  normal  n/a   
 
 
-
 
NC_009376  Pars_0541  hypothetical protein  41.38 
 
 
149 aa  57.8  0.00000007  Pyrobaculum arsenaticum DSM 13514  Archaea  normal  0.0982669  normal  0.227881 
 
 
-
 
NC_007948  Bpro_4725  hypothetical protein  34.02 
 
 
123 aa  57  0.0000001  Polaromonas sp. JS666  Bacteria  normal  normal 
 
 
-
 
NC_013411  GYMC61_2832  protein of unknown function DUF59  33.33 
 
 
108 aa  57  0.0000001  Geobacillus sp. Y412MC61  Bacteria  n/a    n/a   
 
 
-
 
NC_013061  Phep_3035  FeS assembly SUF system protein  34.65 
 
 
104 aa  57  0.0000001  Pedobacter heparinus DSM 2366  Bacteria  normal  normal  0.010077 
 
 
-
 
NC_006369  lpl0641  hypothetical protein  34 
 
 
111 aa  55.1  0.0000005  Legionella pneumophila str. Lens  Bacteria  n/a    n/a   
 
 
-
 
NC_002950  PG1777  hypothetical protein  35.79 
 
 
105 aa  54.7  0.0000006  Porphyromonas gingivalis W83  Bacteria  n/a    normal 
 
 
-
 
NC_006368  lpp0657  hypothetical protein  34 
 
 
111 aa  54.3  0.0000007  Legionella pneumophila str. Paris  Bacteria  n/a    n/a   
 
 
-
 
NC_010655  Amuc_1600  FeS assembly SUF system protein SufT  39.09 
 
 
181 aa  53.9  0.000001  Akkermansia muciniphila ATCC BAA-835  Bacteria  normal  normal 
 
 
-
 
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