More than 300 homologs were found in PanDaTox collection
for query gene Lcho_3301 on replicon NC_010524
Organism: Leptothrix cholodnii SP-6



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Replicon accession

Locus tag

Product

% identity

Alignment on query gene

Alignment on homolog gene

Length

Bit score

E-value

Organism name

Kingdom

Plasmid clonability

Plasmid unclonability p-value

Fosmid clonability

Fosmid unclonability p-value

Coverage Plot

PanDaTox homologs

Experimental validation
NC_010524  Lcho_3301  binding-protein-dependent transport systems inner membrane component  100 
 
 
291 aa  580  1.0000000000000001e-165  Leptothrix cholodnii SP-6  Bacteria  n/a    normal 
 
 
-
 
NC_009654  Mmwyl1_0230  binding-protein-dependent transport systems inner membrane component  67.37 
 
 
292 aa  416  9.999999999999999e-116  Marinomonas sp. MWYL1  Bacteria  normal  normal 
 
 
-
 
NC_009921  Franean1_7037  binding-protein-dependent transport systems inner membrane component  54.48 
 
 
309 aa  325  6e-88  Frankia sp. EAN1pec  Bacteria  normal  normal 
 
 
-
 
NC_013235  Namu_3849  binding-protein-dependent transport systems inner membrane component  54.68 
 
 
301 aa  310  2e-83  Nakamurella multipartita DSM 44233  Bacteria  normal  0.107146  normal 
 
 
-
 
NC_009972  Haur_1152  binding-protein-dependent transport systems inner membrane component  47.84 
 
 
296 aa  274  1.0000000000000001e-72  Herpetosiphon aurantiacus ATCC 23779  Bacteria  normal  0.500307  n/a   
 
 
-
 
NC_008781  Pnap_0230  binding-protein-dependent transport systems inner membrane component  44.44 
 
 
298 aa  252  6e-66  Polaromonas naphthalenivorans CJ2  Bacteria  normal  normal  0.4668 
 
 
-
 
NC_011899  Hore_15540  binding-protein-dependent transport systems inner membrane component  41.02 
 
 
277 aa  212  4.9999999999999996e-54  Halothermothrix orenii H 168  Bacteria  normal  n/a   
 
 
-
 
NC_009939  Dgeo_2917  ABC-type sugar transport system, permease component  39.92 
 
 
273 aa  174  1.9999999999999998e-42  Deinococcus geothermalis DSM 11300  Bacteria  n/a    n/a   
 
 
-
 
NC_013521  Sked_21170  carbohydrate ABC transporter membrane protein  36.27 
 
 
302 aa  171  1e-41  Sanguibacter keddieii DSM 10542  Bacteria  normal  0.281989  normal  0.140209 
 
 
-
 
NC_012848  Rleg_4873  binding-protein-dependent transport systems inner membrane component  38.55 
 
 
297 aa  170  3e-41  Rhizobium leguminosarum bv. trifolii WSM1325  Bacteria  normal  0.978062  normal  0.531607 
 
 
-
 
NC_014213  Mesil_3420  proteophosphoglycan ppg4  35.94 
 
 
272 aa  169  6e-41  Meiothermus silvanus DSM 9946  Bacteria  normal  0.257772  normal 
 
 
-
 
NC_014213  Mesil_3228  binding-protein-dependent transport systems inner membrane component  35.94 
 
 
272 aa  169  6e-41  Meiothermus silvanus DSM 9946  Bacteria  decreased coverage  0.00685083  normal 
 
 
-
 
NC_010320  Teth514_2199  monosaccharide-transporting ATPase  34.52 
 
 
269 aa  167  1e-40  Thermoanaerobacter sp. X514  Bacteria  normal  0.139368  n/a   
 
 
-
 
NC_010003  Pmob_0719  binding-protein-dependent transport systems inner membrane component  35.83 
 
 
279 aa  164  2.0000000000000002e-39  Petrotoga mobilis SJ95  Bacteria  normal  n/a   
 
 
-
 
NC_013205  Aaci_0915  binding-protein-dependent transport systems inner membrane component  31.71 
 
 
280 aa  162  5.0000000000000005e-39  Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446  Bacteria  normal  n/a   
 
 
-
 
NC_012669  Bcav_0791  binding-protein-dependent transport systems inner membrane component  33.56 
 
 
308 aa  162  7e-39  Beutenbergia cavernae DSM 12333  Bacteria  normal  normal  0.013285 
 
 
-
 
NC_011899  Hore_20610  binding-protein-dependent transport systems inner membrane component  34.86 
 
 
273 aa  162  8.000000000000001e-39  Halothermothrix orenii H 168  Bacteria  normal  n/a   
 
 
-
 
NC_009718  Fnod_0396  binding-protein-dependent transport systems inner membrane component  34.65 
 
 
722 aa  159  6e-38  Fervidobacterium nodosum Rt17-B1  Bacteria  normal  0.0626826  n/a   
 
 
-
 
NC_013235  Namu_0512  binding-protein-dependent transport systems inner membrane component  35.38 
 
 
298 aa  158  9e-38  Nakamurella multipartita DSM 44233  Bacteria  normal  normal 
 
 
-
 
NC_008528  OEOE_1458  ABC-type sugar transport system, permease component  34.39 
 
 
276 aa  158  1e-37  Oenococcus oeni PSU-1  Bacteria  normal  n/a   
 
 
-
 
NC_010003  Pmob_0287  binding-protein-dependent transport systems inner membrane component  35 
 
 
279 aa  158  1e-37  Petrotoga mobilis SJ95  Bacteria  normal  0.0553602  n/a   
 
 
-
 
NC_008541  Arth_3148  binding-protein-dependent transport systems inner membrane component  32.51 
 
 
311 aa  157  2e-37  Arthrobacter sp. FB24  Bacteria  decreased coverage  0.00417428  n/a   
 
 
-
 
NC_009636  Smed_1700  binding-protein-dependent transport systems inner membrane component  30.77 
 
 
281 aa  157  2e-37  Sinorhizobium medicae WSM419  Bacteria  normal  normal 
 
 
-
 
NC_013411  GYMC61_2846  binding-protein-dependent transport systems inner membrane component  32.96 
 
 
270 aa  155  6e-37  Geobacillus sp. Y412MC61  Bacteria  n/a    n/a   
 
 
-
 
NC_013739  Cwoe_3981  binding-protein-dependent transport systems inner membrane component  30.21 
 
 
274 aa  155  9e-37  Conexibacter woesei DSM 14684  Bacteria  normal  0.270796  normal 
 
 
-
 
NC_014212  Mesil_2464  binding-protein-dependent transport systems inner membrane component  33.57 
 
 
270 aa  154  1e-36  Meiothermus silvanus DSM 9946  Bacteria  normal  0.668515  normal  0.267306 
 
 
-
 
NC_013526  Tter_2572  binding-protein-dependent transport systems inner membrane component  34.58 
 
 
281 aa  153  2.9999999999999998e-36  Thermobaculum terrenum ATCC BAA-798  Bacteria  normal  n/a   
 
 
-
 
NC_012793  GWCH70_1391  binding-protein-dependent transport systems inner membrane component  32.98 
 
 
270 aa  153  2.9999999999999998e-36  Geobacillus sp. WCH70  Bacteria  normal  n/a   
 
 
-
 
NC_010483  TRQ2_1694  binding-protein-dependent transport systems inner membrane component  34.05 
 
 
276 aa  153  2.9999999999999998e-36  Thermotoga sp. RQ2  Bacteria  normal  n/a   
 
 
-
 
NC_008010  Dgeo_2724  binding-protein-dependent transport systems inner membrane component  33.11 
 
 
290 aa  152  4e-36  Deinococcus geothermalis DSM 11300  Bacteria  normal  0.191447  n/a   
 
 
-
 
NC_013530  Xcel_2649  binding-protein-dependent transport systems inner membrane component  31.69 
 
 
300 aa  153  4e-36  Xylanimonas cellulosilytica DSM 15894  Bacteria  normal  n/a   
 
 
-
 
NC_011831  Cagg_0727  binding-protein-dependent transport systems inner membrane component  34.98 
 
 
279 aa  152  5e-36  Chloroflexus aggregans DSM 9485  Bacteria  hitchhiker  0.000188602  normal 
 
 
-
 
NC_012669  Bcav_1524  binding-protein-dependent transport systems inner membrane component  32.7 
 
 
298 aa  152  5e-36  Beutenbergia cavernae DSM 12333  Bacteria  normal  normal 
 
 
-
 
NC_011725  BCB4264_A0605  putative glycerol-3-phosphate ABC transporter, permease protein  30.28 
 
 
273 aa  152  5e-36  Bacillus cereus B4264  Bacteria  normal  n/a   
 
 
-
 
NC_003909  BCE_0631  glycerol-3-phosphate ABC transporter, permease protein, putative  30.28 
 
 
273 aa  152  5.9999999999999996e-36  Bacillus cereus ATCC 10987  Bacteria  normal  n/a   
 
 
-
 
NC_011658  BCAH187_A0699  putative glycerol-3-phosphate ABC transporter, permease protein  30.28 
 
 
273 aa  152  5.9999999999999996e-36  Bacillus cereus AH187  Bacteria  normal  n/a   
 
 
-
 
NC_011772  BCG9842_B4734  putative glycerol-3-phosphate ABC transporter, permease protein  30.28 
 
 
273 aa  152  5.9999999999999996e-36  Bacillus cereus G9842  Bacteria  normal  normal 
 
 
-
 
NC_008709  Ping_2510  ABC sugar transporter inner membrane binding protein  34.56 
 
 
278 aa  152  7e-36  Psychromonas ingrahamii 37  Bacteria  normal  normal  0.686515 
 
 
-
 
NC_010184  BcerKBAB4_0482  binding-protein-dependent transport systems inner membrane component  30.28 
 
 
273 aa  152  8.999999999999999e-36  Bacillus weihenstephanensis KBAB4  Bacteria  normal  n/a   
 
 
-
 
NC_005945  BAS0537  glycerol-3-phosphate ABC transporter permease  30.28 
 
 
273 aa  151  1e-35  Bacillus anthracis str. Sterne  Bacteria  normal  n/a   
 
 
-
 
NC_005957  BT9727_0479  glycerol-3-phosphate ABC transporter, permease  30.28 
 
 
273 aa  151  1e-35  Bacillus thuringiensis serovar konkukian str. 97-27  Bacteria  normal  n/a   
 
 
-
 
NC_006274  BCZK0481  glycerol-3-phosphate ABC transporter, permease  30.28 
 
 
273 aa  151  1e-35  Bacillus cereus E33L  Bacteria  normal  n/a   
 
 
-
 
NC_007530  GBAA_0568  glycerol-3-phosphate ABC transporter permease protein  30.28 
 
 
273 aa  151  1e-35  Bacillus anthracis str. 'Ames Ancestor'  Bacteria  normal  n/a   
 
 
-
 
NC_011773  BCAH820_0624  putative glycerol-3-phosphate ABC transporter, permease protein  30.28 
 
 
273 aa  151  1e-35  Bacillus cereus AH820  Bacteria  n/a    normal 
 
 
-
 
NC_013946  Mrub_0043  binding-protein-dependent transport systems inner membrane component  33.1 
 
 
270 aa  149  4e-35  Meiothermus ruber DSM 1279  Bacteria  normal  normal 
 
 
-
 
NC_009616  Tmel_1618  binding-protein-dependent transport systems inner membrane component  37.32 
 
 
198 aa  149  5e-35  Thermosipho melanesiensis BI429  Bacteria  normal  n/a   
 
 
-
 
NC_013595  Sros_2366  binding-protein-dependent transport systems inner membrane component  32.04 
 
 
288 aa  149  5e-35  Streptosporangium roseum DSM 43021  Bacteria  normal  0.125554  normal 
 
 
-
 
NC_011881  Achl_4623  binding-protein-dependent transport systems inner membrane component  31.06 
 
 
285 aa  149  6e-35  Arthrobacter chlorophenolicus A6  Bacteria  n/a    normal 
 
 
-
 
NC_009636  Smed_2313  binding-protein-dependent transport systems inner membrane component  33.7 
 
 
280 aa  149  7e-35  Sinorhizobium medicae WSM419  Bacteria  normal  0.0326071  normal  0.242877 
 
 
-
 
NC_013521  Sked_05240  carbohydrate ABC transporter membrane protein  32.4 
 
 
312 aa  148  8e-35  Sanguibacter keddieii DSM 10542  Bacteria  normal  normal 
 
 
-
 
NC_013131  Caci_4318  binding-protein-dependent transport systems inner membrane component  34.29 
 
 
295 aa  148  9e-35  Catenulispora acidiphila DSM 44928  Bacteria  normal  0.574995  hitchhiker  0.00173435 
 
 
-
 
NC_012669  Bcav_1700  binding-protein-dependent transport systems inner membrane component  35.47 
 
 
293 aa  148  1.0000000000000001e-34  Beutenbergia cavernae DSM 12333  Bacteria  normal  normal 
 
 
-
 
NC_013526  Tter_2216  binding-protein-dependent transport systems inner membrane component  32.16 
 
 
301 aa  147  1.0000000000000001e-34  Thermobaculum terrenum ATCC BAA-798  Bacteria  normal  n/a   
 
 
-
 
NC_010571  Oter_4150  binding-protein-dependent transport systems inner membrane component  30.14 
 
 
274 aa  147  2.0000000000000003e-34  Opitutus terrae PB90-1  Bacteria  normal  0.0262068  normal  0.102994 
 
 
-
 
NC_014212  Mesil_0956  binding-protein-dependent transport systems inner membrane component  34.47 
 
 
286 aa  147  3e-34  Meiothermus silvanus DSM 9946  Bacteria  normal  normal  0.143013 
 
 
-
 
NC_013525  Tter_0831  binding-protein-dependent transport systems inner membrane component  34.14 
 
 
310 aa  146  3e-34  Thermobaculum terrenum ATCC BAA-798  Bacteria  n/a    n/a   
 
 
-
 
NC_012669  Bcav_3574  Monosaccharide-transporting ATPase  34.52 
 
 
290 aa  146  4.0000000000000006e-34  Beutenbergia cavernae DSM 12333  Bacteria  normal  0.156852  normal 
 
 
-
 
NC_009486  Tpet_0320  monosaccharide-transporting ATPase  33.77 
 
 
701 aa  146  5e-34  Thermotoga petrophila RKU-1  Bacteria  normal  n/a   
 
 
-
 
NC_012034  Athe_0401  binding-protein-dependent transport systems inner membrane component  30.25 
 
 
277 aa  145  5e-34  Anaerocellum thermophilum DSM 6725  Bacteria  unclonable  0.000000000457855  n/a   
 
 
-
 
NC_013205  Aaci_0794  binding-protein-dependent transport systems inner membrane component  32.85 
 
 
273 aa  145  7.0000000000000006e-34  Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446  Bacteria  normal  0.256296  n/a   
 
 
-
 
NC_009636  Smed_3327  binding-protein-dependent transport systems inner membrane component  33.7 
 
 
295 aa  145  8.000000000000001e-34  Sinorhizobium medicae WSM419  Bacteria  normal  normal  0.925961 
 
 
-
 
NC_013526  Tter_2293  binding-protein-dependent transport systems inner membrane component  29.08 
 
 
297 aa  145  9e-34  Thermobaculum terrenum ATCC BAA-798  Bacteria  normal  n/a   
 
 
-
 
NC_007333  Tfu_1703  putative transport system permease ABC transporter protein  32 
 
 
292 aa  144  1e-33  Thermobifida fusca YX  Bacteria  normal  0.757038  n/a   
 
 
-
 
NC_008699  Noca_0043  binding-protein-dependent transport systems inner membrane component  32.16 
 
 
290 aa  144  1e-33  Nocardioides sp. JS614  Bacteria  normal  n/a   
 
 
-
 
NC_010320  Teth514_1101  binding-protein-dependent transport systems inner membrane component  31.52 
 
 
280 aa  144  1e-33  Thermoanaerobacter sp. X514  Bacteria  normal  n/a   
 
 
-
 
NC_012669  Bcav_2601  binding-protein-dependent transport systems inner membrane component  32.86 
 
 
275 aa  144  1e-33  Beutenbergia cavernae DSM 12333  Bacteria  normal  0.260696  normal 
 
 
-
 
NC_013739  Cwoe_4881  binding-protein-dependent transport systems inner membrane component  32.04 
 
 
295 aa  145  1e-33  Conexibacter woesei DSM 14684  Bacteria  normal  0.48115  normal 
 
 
-
 
NC_014210  Ndas_2035  binding-protein-dependent transport systems inner membrane component  31.94 
 
 
291 aa  145  1e-33  Nocardiopsis dassonvillei subsp. dassonvillei DSM 43111  Bacteria  normal  0.0974901  normal  0.381629 
 
 
-
 
NC_011369  Rleg2_4017  binding-protein-dependent transport systems inner membrane component  32.06 
 
 
292 aa  144  2e-33  Rhizobium leguminosarum bv. trifolii WSM2304  Bacteria  normal  0.924355  normal 
 
 
-
 
NC_013947  Snas_2735  binding-protein-dependent transport systems inner membrane component  30.85 
 
 
268 aa  143  3e-33  Stackebrandtia nassauensis DSM 44728  Bacteria  normal  0.146401  normal  0.117975 
 
 
-
 
NC_011886  Achl_3129  binding-protein-dependent transport systems inner membrane component  33.09 
 
 
313 aa  143  3e-33  Arthrobacter chlorophenolicus A6  Bacteria  n/a    normal  0.0180019 
 
 
-
 
NC_012669  Bcav_3989  binding-protein-dependent transport systems inner membrane component  31.34 
 
 
324 aa  143  3e-33  Beutenbergia cavernae DSM 12333  Bacteria  normal  normal 
 
 
-
 
NC_014212  Mesil_2056  binding-protein-dependent transport systems inner membrane component  33.33 
 
 
406 aa  143  4e-33  Meiothermus silvanus DSM 9946  Bacteria  normal  0.0947722  normal  0.449211 
 
 
-
 
NC_009616  Tmel_0751  binding-protein-dependent transport systems inner membrane component  31.47 
 
 
277 aa  142  5e-33  Thermosipho melanesiensis BI429  Bacteria  normal  n/a   
 
 
-
 
NC_013172  Bfae_29710  carbohydrate ABC transporter membrane protein  29.93 
 
 
304 aa  142  5e-33  Brachybacterium faecium DSM 4810  Bacteria  normal  0.942295  n/a   
 
 
-
 
NC_012669  Bcav_1507  binding-protein-dependent transport systems inner membrane component  28.98 
 
 
303 aa  142  7e-33  Beutenbergia cavernae DSM 12333  Bacteria  normal  0.659062  normal 
 
 
-
 
NC_012850  Rleg_4346  binding-protein-dependent transport systems inner membrane component  32.16 
 
 
292 aa  142  8e-33  Rhizobium leguminosarum bv. trifolii WSM1325  Bacteria  normal  hitchhiker  0.0000934569 
 
 
-
 
NC_007413  Ava_2094  binding-protein dependent transport system inner membrane protein  30.04 
 
 
279 aa  142  8e-33  Anabaena variabilis ATCC 29413  Bacteria  normal  0.179988  hitchhiker  0.00000184258 
 
 
-
 
NC_010816  BLD_1550  MalG-type ABC sugar transport system permease component  29.93 
 
 
308 aa  142  8e-33  Bifidobacterium longum DJO10A  Bacteria  normal  n/a   
 
 
-
 
NC_008048  Sala_0749  binding-protein-dependent transport systems inner membrane component  32.1 
 
 
278 aa  142  9e-33  Sphingopyxis alaskensis RB2256  Bacteria  normal  normal 
 
 
-
 
NC_008262  CPR_0906  binding-protein-dependent transporter inner membrane protein  30.15 
 
 
283 aa  141  9.999999999999999e-33  Clostridium perfringens SM101  Bacteria  normal  0.100399  n/a   
 
 
-
 
NC_013526  Tter_2528  binding-protein-dependent transport systems inner membrane component  33.46 
 
 
312 aa  141  9.999999999999999e-33  Thermobaculum terrenum ATCC BAA-798  Bacteria  normal  n/a   
 
 
-
 
NC_013526  Tter_2040  binding-protein-dependent transport systems inner membrane component  30.56 
 
 
305 aa  141  9.999999999999999e-33  Thermobaculum terrenum ATCC BAA-798  Bacteria  hitchhiker  0.00998336  n/a   
 
 
-
 
NC_011769  DvMF_0658  binding-protein-dependent transport systems inner membrane component  31.37 
 
 
277 aa  141  9.999999999999999e-33  Desulfovibrio vulgaris str. 'Miyazaki F'  Bacteria  n/a    normal  0.390959 
 
 
-
 
NC_013525  Tter_0124  binding-protein-dependent transport systems inner membrane component  32.87 
 
 
277 aa  140  1.9999999999999998e-32  Thermobaculum terrenum ATCC BAA-798  Bacteria  n/a    n/a   
 
 
-
 
NC_013526  Tter_2207  binding-protein-dependent transport systems inner membrane component  29.73 
 
 
299 aa  140  1.9999999999999998e-32  Thermobaculum terrenum ATCC BAA-798  Bacteria  normal  n/a   
 
 
-
 
NC_011886  Achl_0497  binding-protein-dependent transport systems inner membrane component  28.37 
 
 
306 aa  140  1.9999999999999998e-32  Arthrobacter chlorophenolicus A6  Bacteria  n/a    hitchhiker  0.000825086 
 
 
-
 
NC_013530  Xcel_2536  binding-protein-dependent transport systems inner membrane component  31.75 
 
 
306 aa  140  1.9999999999999998e-32  Xylanimonas cellulosilytica DSM 15894  Bacteria  normal  0.250656  n/a   
 
 
-
 
NC_008541  Arth_3327  binding-protein-dependent transport systems inner membrane component  32.72 
 
 
314 aa  140  3e-32  Arthrobacter sp. FB24  Bacteria  hitchhiker  0.00328886  n/a   
 
 
-
 
NC_013411  GYMC61_1729  binding-protein-dependent transport systems inner membrane component  33.45 
 
 
275 aa  140  3e-32  Geobacillus sp. Y412MC61  Bacteria  n/a    n/a   
 
 
-
 
NC_011369  Rleg2_1701  binding-protein-dependent transport systems inner membrane component  34.01 
 
 
349 aa  140  3e-32  Rhizobium leguminosarum bv. trifolii WSM2304  Bacteria  normal  0.59414  normal 
 
 
-
 
NC_014213  Mesil_3392  fumarate reductase/succinate dehydrogenase flavoprotein domain protein  31.62 
 
 
278 aa  139  3.9999999999999997e-32  Meiothermus silvanus DSM 9946  Bacteria  normal  0.196448  normal  0.954202 
 
 
-
 
NC_013172  Bfae_06290  carbohydrate ABC transporter membrane protein  33.46 
 
 
291 aa  140  3.9999999999999997e-32  Brachybacterium faecium DSM 4810  Bacteria  normal  n/a   
 
 
-
 
NC_010483  TRQ2_0338  monosaccharide-transporting ATPase  33.33 
 
 
701 aa  140  3.9999999999999997e-32  Thermotoga sp. RQ2  Bacteria  normal  n/a   
 
 
-
 
NC_013174  Jden_2243  binding-protein-dependent transport systems inner membrane component  32.26 
 
 
307 aa  139  4.999999999999999e-32  Jonesia denitrificans DSM 20603  Bacteria  normal  normal  0.556058 
 
 
-
 
NC_010003  Pmob_1378  binding-protein-dependent transport systems inner membrane component  34.73 
 
 
743 aa  139  4.999999999999999e-32  Petrotoga mobilis SJ95  Bacteria  normal  0.445152  n/a   
 
 
-
 
NC_013131  Caci_4695  binding-protein-dependent transport systems inner membrane component  31.33 
 
 
271 aa  139  6e-32  Catenulispora acidiphila DSM 44928  Bacteria  hitchhiker  0.00258755  normal  0.655339 
 
 
-
 
NC_012669  Bcav_0802  binding-protein-dependent transport systems inner membrane component  30.82 
 
 
286 aa  139  6e-32  Beutenbergia cavernae DSM 12333  Bacteria  normal  normal  0.0123708 
 
 
-
 
NC_013947  Snas_0205  binding-protein-dependent transport systems inner membrane component  31.56 
 
 
279 aa  139  6e-32  Stackebrandtia nassauensis DSM 44728  Bacteria  normal  0.67338  normal  0.16615 
 
 
-
 
NC_009565  TBFG_12849  sn-glycerol-3-phosphate transport integral membrane protein ABC transporter ugpE  28.42 
 
 
275 aa  139  7.999999999999999e-32  Mycobacterium tuberculosis F11  Bacteria  hitchhiker  0.00000000000144305  normal 
 
 
-
 
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