More than 300 homologs were found in PanDaTox collection
for query gene Tpau_1200 on replicon NC_014158
Organism: Tsukamurella paurometabola DSM 20162



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Replicon accession

Locus tag

Product

% identity

Alignment on query gene

Alignment on homolog gene

Length

Bit score

E-value

Organism name

Kingdom

Plasmid clonability

Plasmid unclonability p-value

Fosmid clonability

Fosmid unclonability p-value

Coverage Plot

PanDaTox homologs

Experimental validation
NC_014158  Tpau_1200  binding-protein-dependent transport systems inner membrane component  100 
 
 
310 aa  613  9.999999999999999e-175  Tsukamurella paurometabola DSM 20162  Bacteria  normal  n/a   
 
 
-
 
NC_013441  Gbro_1867  binding-protein-dependent transport systems inner membrane component  78.42 
 
 
388 aa  446  1.0000000000000001e-124  Gordonia bronchialis DSM 43247  Bacteria  normal  n/a   
 
 
-
 
NC_008146  Mmcs_3982  binding-protein-dependent transport systems inner membrane component  75 
 
 
308 aa  436  1e-121  Mycobacterium sp. MCS  Bacteria  normal  n/a   
 
 
-
 
NC_008705  Mkms_4056  binding-protein-dependent transport systems inner membrane component  75 
 
 
308 aa  436  1e-121  Mycobacterium sp. KMS  Bacteria  normal  0.853442  normal  0.685254 
 
 
-
 
NC_009077  Mjls_3996  binding-protein-dependent transport systems inner membrane component  75 
 
 
308 aa  436  1e-121  Mycobacterium sp. JLS  Bacteria  normal  0.172958  normal  0.378531 
 
 
-
 
NC_013159  Svir_06500  carbohydrate ABC transporter membrane protein  69.77 
 
 
321 aa  422  1e-117  Saccharomonospora viridis DSM 43017  Bacteria  normal  normal 
 
 
-
 
NC_009565  TBFG_11261  sugar-transport integral membrane protein ABC transporter sugA  69.28 
 
 
307 aa  415  9.999999999999999e-116  Mycobacterium tuberculosis F11  Bacteria  normal  hitchhiker  0.00812399 
 
 
-
 
NC_013093  Amir_0808  binding-protein-dependent transport systems inner membrane component  66.45 
 
 
318 aa  392  1e-108  Actinosynnema mirum DSM 43827  Bacteria  normal  n/a   
 
 
-
 
NC_013947  Snas_4645  binding-protein-dependent transport systems inner membrane component  64.48 
 
 
299 aa  379  1e-104  Stackebrandtia nassauensis DSM 44728  Bacteria  normal  hitchhiker  0.00190071 
 
 
-
 
NC_009921  Franean1_4616  binding-protein-dependent transport systems inner membrane component  66.89 
 
 
331 aa  353  2e-96  Frankia sp. EAN1pec  Bacteria  normal  0.338244  normal 
 
 
-
 
NC_013947  Snas_1042  binding-protein-dependent transport systems inner membrane component  63.99 
 
 
292 aa  348  6e-95  Stackebrandtia nassauensis DSM 44728  Bacteria  normal  0.0233696  normal  0.724909 
 
 
-
 
NC_007484  Noc_0281  ABC transporter, inner membrane subunit  55.05 
 
 
292 aa  299  4e-80  Nitrosococcus oceani ATCC 19707  Bacteria  normal  n/a   
 
 
-
 
NC_013169  Ksed_20480  carbohydrate ABC transporter membrane protein  51.9 
 
 
318 aa  291  1e-77  Kytococcus sedentarius DSM 20547  Bacteria  normal  0.0521728  normal  0.389193 
 
 
-
 
NC_009664  Krad_1114  binding-protein-dependent transport systems inner membrane component  48.26 
 
 
319 aa  277  1e-73  Kineococcus radiotolerans SRS30216  Bacteria  normal  0.32149  normal  0.356013 
 
 
-
 
NC_012669  Bcav_1096  binding-protein-dependent transport systems inner membrane component  50.34 
 
 
315 aa  277  2e-73  Beutenbergia cavernae DSM 12333  Bacteria  normal  0.175054  normal 
 
 
-
 
NC_009338  Mflv_3852  binding-protein-dependent transport systems inner membrane component  50 
 
 
310 aa  275  6e-73  Mycobacterium gilvum PYR-GCK  Bacteria  normal  normal  0.0634568 
 
 
-
 
NC_008726  Mvan_2543  binding-protein-dependent transport systems inner membrane component  49.21 
 
 
310 aa  272  4.0000000000000004e-72  Mycobacterium vanbaalenii PYR-1  Bacteria  normal  0.944367  normal 
 
 
-
 
NC_013757  Gobs_3933  binding-protein-dependent transport systems inner membrane component  47.19 
 
 
320 aa  267  1e-70  Geodermatophilus obscurus DSM 43160  Bacteria  normal  0.292649  n/a   
 
 
-
 
NC_014151  Cfla_0961  binding-protein-dependent transport systems inner membrane component  52.96 
 
 
320 aa  257  1e-67  Cellulomonas flavigena DSM 20109  Bacteria  normal  0.158412  hitchhiker  0.000108715 
 
 
-
 
NC_012803  Mlut_04590  permease component of ABC-type sugar transporter  50.17 
 
 
323 aa  251  1e-65  Micrococcus luteus NCTC 2665  Bacteria  normal  0.359187  n/a   
 
 
-
 
NC_008541  Arth_3686  binding-protein-dependent transport systems inner membrane component  41.58 
 
 
310 aa  239  5e-62  Arthrobacter sp. FB24  Bacteria  normal  n/a   
 
 
-
 
NC_013411  GYMC61_2958  binding-protein-dependent transport systems inner membrane component  45.24 
 
 
435 aa  215  8e-55  Geobacillus sp. Y412MC61  Bacteria  n/a    n/a   
 
 
-
 
NC_010320  Teth514_0396  binding-protein-dependent transport systems inner membrane component  43.75 
 
 
307 aa  215  9e-55  Thermoanaerobacter sp. X514  Bacteria  normal  0.701562  n/a   
 
 
-
 
NC_013525  Tter_0398  binding-protein-dependent transport systems inner membrane component  37.24 
 
 
312 aa  202  9e-51  Thermobaculum terrenum ATCC BAA-798  Bacteria  n/a    n/a   
 
 
-
 
NC_012034  Athe_2309  binding-protein-dependent transport systems inner membrane component  42.8 
 
 
427 aa  196  5.000000000000001e-49  Anaerocellum thermophilum DSM 6725  Bacteria  hitchhiker  0.00000129291  n/a   
 
 
-
 
NC_009675  Anae109_1696  binding-protein-dependent transport systems inner membrane component  42.96 
 
 
291 aa  195  7e-49  Anaeromyxobacter sp. Fw109-5  Bacteria  hitchhiker  0.00180742  normal 
 
 
-
 
NC_009832  Spro_2525  binding-protein-dependent transport systems inner membrane component  39.34 
 
 
330 aa  194  1e-48  Serratia proteamaculans 568  Bacteria  normal  normal 
 
 
-
 
NC_008148  Rxyl_1990  binding-protein-dependent transport systems inner membrane component  36.77 
 
 
306 aa  195  1e-48  Rubrobacter xylanophilus DSM 9941  Bacteria  normal  0.306567  n/a   
 
 
-
 
NC_014210  Ndas_3354  binding-protein-dependent transport systems inner membrane component  37.38 
 
 
299 aa  194  2e-48  Nocardiopsis dassonvillei subsp. dassonvillei DSM 43111  Bacteria  normal  normal 
 
 
-
 
NC_011729  PCC7424_1896  binding-protein-dependent transport systems inner membrane component  36.59 
 
 
296 aa  188  1e-46  Cyanothece sp. PCC 7424  Bacteria  n/a    hitchhiker  1.0543799999999999e-20 
 
 
-
 
NC_011368  Rleg2_5492  binding-protein-dependent transport systems inner membrane component  34.95 
 
 
328 aa  186  3e-46  Rhizobium leguminosarum bv. trifolii WSM2304  Bacteria  normal  0.511246  normal  0.17987 
 
 
-
 
NC_009668  Oant_2941  binding-protein-dependent transport systems inner membrane component  34.56 
 
 
320 aa  184  2.0000000000000003e-45  Ochrobactrum anthropi ATCC 49188  Bacteria  normal  0.974377  n/a   
 
 
-
 
NC_009616  Tmel_1819  binding-protein-dependent transport systems inner membrane component  37.15 
 
 
293 aa  184  2.0000000000000003e-45  Thermosipho melanesiensis BI429  Bacteria  normal  0.215175  n/a   
 
 
-
 
NC_009654  Mmwyl1_0024  binding-protein-dependent transport systems inner membrane component  34.8 
 
 
320 aa  182  5.0000000000000004e-45  Marinomonas sp. MWYL1  Bacteria  normal  0.227719  normal  0.433481 
 
 
-
 
NC_010003  Pmob_0028  binding-protein-dependent transport systems inner membrane component  38.68 
 
 
300 aa  182  6e-45  Petrotoga mobilis SJ95  Bacteria  unclonable  0.0000000378352  n/a   
 
 
-
 
NC_009718  Fnod_0449  binding-protein-dependent transport systems inner membrane component  36.46 
 
 
295 aa  182  8.000000000000001e-45  Fervidobacterium nodosum Rt17-B1  Bacteria  normal  0.982578  n/a   
 
 
-
 
NC_010483  TRQ2_0971  binding-protein-dependent transport systems inner membrane component  37.76 
 
 
293 aa  180  2e-44  Thermotoga sp. RQ2  Bacteria  normal  0.0994152  n/a   
 
 
-
 
NC_009486  Tpet_0955  binding-protein-dependent transport systems inner membrane component  37.76 
 
 
293 aa  180  2e-44  Thermotoga petrophila RKU-1  Bacteria  normal  n/a   
 
 
-
 
NC_009620  Smed_3811  binding-protein-dependent transport systems inner membrane component  36.03 
 
 
328 aa  179  4e-44  Sinorhizobium medicae WSM419  Bacteria  normal  normal 
 
 
-
 
NC_009664  Krad_4016  binding-protein-dependent transport systems inner membrane component  37.31 
 
 
338 aa  179  4.999999999999999e-44  Kineococcus radiotolerans SRS30216  Bacteria  normal  normal  0.0789775 
 
 
-
 
NC_008025  Dgeo_0590  binding-protein-dependent transport systems inner membrane component  40.22 
 
 
315 aa  177  1e-43  Deinococcus geothermalis DSM 11300  Bacteria  normal  0.273483  normal 
 
 
-
 
NC_004311  BRA0305  sugar ABC transporter, permease protein  33.99 
 
 
325 aa  174  1.9999999999999998e-42  Brucella suis 1330  Bacteria  normal  0.0474821  n/a   
 
 
-
 
NC_007963  Csal_0259  binding-protein-dependent transport systems inner membrane component  34.67 
 
 
324 aa  173  2.9999999999999996e-42  Chromohalobacter salexigens DSM 3043  Bacteria  normal  n/a   
 
 
-
 
NC_008687  Pden_4441  binding-protein-dependent transport systems inner membrane component  34.46 
 
 
320 aa  173  2.9999999999999996e-42  Paracoccus denitrificans PD1222  Bacteria  normal  0.0272526  normal 
 
 
-
 
NC_009504  BOV_A0280  sugar ABC transporter, permease protein  33.99 
 
 
325 aa  173  2.9999999999999996e-42  Brucella ovis ATCC 25840  Bacteria  normal  n/a   
 
 
-
 
NC_013530  Xcel_2796  binding-protein-dependent transport systems inner membrane component  38 
 
 
355 aa  172  6.999999999999999e-42  Xylanimonas cellulosilytica DSM 15894  Bacteria  normal  n/a   
 
 
-
 
NC_013440  Hoch_3145  binding-protein-dependent transport systems inner membrane component  35.61 
 
 
303 aa  172  9e-42  Haliangium ochraceum DSM 14365  Bacteria  normal  normal  0.236219 
 
 
-
 
NC_011886  Achl_0624  binding-protein-dependent transport systems inner membrane component  36.36 
 
 
345 aa  169  6e-41  Arthrobacter chlorophenolicus A6  Bacteria  n/a    normal  0.65014 
 
 
-
 
NC_013205  Aaci_2236  binding-protein-dependent transport systems inner membrane component  37.41 
 
 
315 aa  169  7e-41  Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446  Bacteria  normal  n/a   
 
 
-
 
NC_011899  Hore_19880  binding-protein-dependent transport systems inner membrane component  34.22 
 
 
323 aa  168  1e-40  Halothermothrix orenii H 168  Bacteria  normal  n/a   
 
 
-
 
NC_012850  Rleg_2284  binding-protein-dependent transport systems inner membrane component  33.33 
 
 
291 aa  164  2.0000000000000002e-39  Rhizobium leguminosarum bv. trifolii WSM1325  Bacteria  normal  normal  0.257134 
 
 
-
 
NC_008686  Pden_1048  binding-protein-dependent transport systems inner membrane component  35.29 
 
 
308 aa  163  4.0000000000000004e-39  Paracoccus denitrificans PD1222  Bacteria  normal  normal 
 
 
-
 
NC_007005  Psyr_0761  binding-protein dependent transport system inner membrane protein  36.46 
 
 
320 aa  161  1e-38  Pseudomonas syringae pv. syringae B728a  Bacteria  normal  0.0629996  normal  0.824676 
 
 
-
 
NC_007604  Synpcc7942_0949  permease protein of sugar ABC transporter  36.19 
 
 
292 aa  161  2e-38  Synechococcus elongatus PCC 7942  Bacteria  normal  0.688345  normal 
 
 
-
 
NC_010003  Pmob_0306  binding-protein-dependent transport systems inner membrane component  36.93 
 
 
294 aa  160  2e-38  Petrotoga mobilis SJ95  Bacteria  normal  n/a   
 
 
-
 
NC_008699  Noca_0870  binding-protein-dependent transport systems inner membrane component  34.04 
 
 
309 aa  157  2e-37  Nocardioides sp. JS614  Bacteria  normal  0.800905  n/a   
 
 
-
 
NC_009620  Smed_4397  binding-protein-dependent transport systems inner membrane component  36.27 
 
 
297 aa  157  2e-37  Sinorhizobium medicae WSM419  Bacteria  normal  normal  0.439093 
 
 
-
 
NC_013946  Mrub_2089  binding-protein-dependent transport systems inner membrane component  40.88 
 
 
293 aa  157  2e-37  Meiothermus ruber DSM 1279  Bacteria  decreased coverage  0.000000240561  normal  0.90976 
 
 
-
 
NC_013131  Caci_2623  binding-protein-dependent transport systems inner membrane component  32.75 
 
 
330 aa  156  3e-37  Catenulispora acidiphila DSM 44928  Bacteria  normal  0.0584633  normal 
 
 
-
 
NC_010524  Lcho_3328  binding-protein-dependent transport systems inner membrane component  32.76 
 
 
304 aa  157  3e-37  Leptothrix cholodnii SP-6  Bacteria  n/a    normal 
 
 
-
 
NC_013744  Htur_4398  binding-protein-dependent transport systems inner membrane component  33.11 
 
 
314 aa  156  5.0000000000000005e-37  Haloterrigena turkmenica DSM 5511  Archaea  normal  0.0144983  n/a   
 
 
-
 
NC_012848  Rleg_5199  binding-protein-dependent transport systems inner membrane component  31.67 
 
 
312 aa  152  7e-36  Rhizobium leguminosarum bv. trifolii WSM1325  Bacteria  normal  normal  0.0867066 
 
 
-
 
NC_013124  Afer_1330  binding-protein-dependent transport systems inner membrane component  34.53 
 
 
316 aa  152  8.999999999999999e-36  Acidimicrobium ferrooxidans DSM 10331  Bacteria  normal  n/a   
 
 
-
 
NC_014165  Tbis_1088  binding-protein-dependent transporter inner membrane component  34.2 
 
 
288 aa  151  2e-35  Thermobispora bispora DSM 43833  Bacteria  normal  0.616528  hitchhiker  0.00755847 
 
 
-
 
NC_008541  Arth_0414  binding-protein-dependent transport systems inner membrane component  38.41 
 
 
345 aa  150  4e-35  Arthrobacter sp. FB24  Bacteria  normal  n/a   
 
 
-
 
NC_009667  Oant_0362  binding-protein-dependent transport systems inner membrane component  33.33 
 
 
312 aa  149  7e-35  Ochrobactrum anthropi ATCC 49188  Bacteria  normal  0.541424  n/a   
 
 
-
 
NC_009636  Smed_2455  binding-protein-dependent transport systems inner membrane component  31.01 
 
 
313 aa  149  8e-35  Sinorhizobium medicae WSM419  Bacteria  normal  normal 
 
 
-
 
NC_004578  PSPTO_0888  sugar ABC transporter, permease protein  36.46 
 
 
320 aa  147  3e-34  Pseudomonas syringae pv. tomato str. DC3000  Bacteria  normal  0.392731  n/a   
 
 
-
 
NC_011368  Rleg2_5469  binding-protein-dependent transport systems inner membrane component  32.47 
 
 
313 aa  146  4.0000000000000006e-34  Rhizobium leguminosarum bv. trifolii WSM2304  Bacteria  normal  normal  0.394829 
 
 
-
 
NC_013202  Hmuk_0943  binding-protein-dependent transport systems inner membrane component  33.22 
 
 
312 aa  146  5e-34  Halomicrobium mukohataei DSM 12286  Archaea  normal  normal  0.301949 
 
 
-
 
NC_012854  Rleg_6527  binding-protein-dependent transport systems inner membrane component  32.89 
 
 
307 aa  146  5e-34  Rhizobium leguminosarum bv. trifolii WSM1325  Bacteria  normal  normal  0.0292278 
 
 
-
 
NC_009708  YpsIP31758_2171  carbohydrate ABC transporter permease  35.52 
 
 
280 aa  145  1e-33  Yersinia pseudotuberculosis IP 31758  Bacteria  normal  0.0580768  n/a   
 
 
-
 
NC_010465  YPK_2279  binding-protein-dependent transport systems inner membrane component  35.55 
 
 
286 aa  144  2e-33  Yersinia pseudotuberculosis YPIII  Bacteria  normal  n/a   
 
 
-
 
NC_013595  Sros_1501  binding-protein-dependent transport systems inner membrane component  32.51 
 
 
304 aa  144  2e-33  Streptosporangium roseum DSM 43021  Bacteria  normal  normal  0.707606 
 
 
-
 
NC_010003  Pmob_0073  binding-protein-dependent transport systems inner membrane component  30.39 
 
 
294 aa  143  4e-33  Petrotoga mobilis SJ95  Bacteria  normal  n/a   
 
 
-
 
NC_011899  Hore_22990  binding-protein-dependent transport systems inner membrane component  32.26 
 
 
286 aa  142  7e-33  Halothermothrix orenii H 168  Bacteria  hitchhiker  0.00000108841  n/a   
 
 
-
 
NC_011369  Rleg2_3668  binding-protein-dependent transport systems inner membrane component  32.28 
 
 
299 aa  141  9.999999999999999e-33  Rhizobium leguminosarum bv. trifolii WSM2304  Bacteria  normal  normal 
 
 
-
 
NC_012669  Bcav_2264  binding-protein-dependent transport systems inner membrane component  32.07 
 
 
307 aa  141  9.999999999999999e-33  Beutenbergia cavernae DSM 12333  Bacteria  normal  0.277747  normal 
 
 
-
 
NC_012848  Rleg_4864  binding-protein-dependent transport systems inner membrane component  30.94 
 
 
319 aa  140  1.9999999999999998e-32  Rhizobium leguminosarum bv. trifolii WSM1325  Bacteria  normal  0.243677  normal  0.263269 
 
 
-
 
NC_010159  YpAngola_A2083  carbohydrate ABC transporter permease  35.16 
 
 
286 aa  141  1.9999999999999998e-32  Yersinia pestis Angola  Bacteria  normal  normal  0.0183943 
 
 
-
 
NC_012848  Rleg_4941  binding-protein-dependent transport systems inner membrane component  32.62 
 
 
309 aa  139  3.9999999999999997e-32  Rhizobium leguminosarum bv. trifolii WSM1325  Bacteria  normal  normal 
 
 
-
 
NC_009485  BBta_7824  putative ABC transporter permease protein  33.46 
 
 
295 aa  140  3.9999999999999997e-32  Bradyrhizobium sp. BTAi1  Bacteria  normal  normal 
 
 
-
 
NC_009832  Spro_2296  binding-protein-dependent transport systems inner membrane component  34.84 
 
 
304 aa  139  6e-32  Serratia proteamaculans 568  Bacteria  normal  0.44429  normal 
 
 
-
 
NC_012850  Rleg_2427  binding-protein-dependent transport systems inner membrane component  29.78 
 
 
322 aa  139  8.999999999999999e-32  Rhizobium leguminosarum bv. trifolii WSM1325  Bacteria  normal  0.535373  normal  0.626108 
 
 
-
 
NC_012669  Bcav_1681  binding-protein-dependent transport systems inner membrane component  30 
 
 
316 aa  138  1e-31  Beutenbergia cavernae DSM 12333  Bacteria  normal  0.130846  normal 
 
 
-
 
NC_009664  Krad_2091  binding-protein-dependent transport systems inner membrane component  33.33 
 
 
292 aa  138  1e-31  Kineococcus radiotolerans SRS30216  Bacteria  normal  normal 
 
 
-
 
NC_009664  Krad_4401  binding-protein-dependent transport systems inner membrane component  30.74 
 
 
316 aa  138  1e-31  Kineococcus radiotolerans SRS30216  Bacteria  normal  normal 
 
 
-
 
NC_010625  Bphy_6370  binding-protein-dependent transport systems inner membrane component  29.86 
 
 
318 aa  137  2e-31  Burkholderia phymatum STM815  Bacteria  normal  normal 
 
 
-
 
NC_007802  Jann_1389  binding-protein-dependent transport systems inner membrane component  33.21 
 
 
300 aa  136  3.0000000000000003e-31  Jannaschia sp. CCS1  Bacteria  normal  0.0114019  normal  0.0892858 
 
 
-
 
NC_009636  Smed_2145  binding-protein-dependent transport systems inner membrane component  33.94 
 
 
362 aa  136  4e-31  Sinorhizobium medicae WSM419  Bacteria  normal  normal 
 
 
-
 
NC_009636  Smed_2698  binding-protein-dependent transport systems inner membrane component  34.2 
 
 
295 aa  136  4e-31  Sinorhizobium medicae WSM419  Bacteria  normal  0.0832027  normal 
 
 
-
 
NC_010676  Bphyt_5637  binding-protein-dependent transport systems inner membrane component  30.77 
 
 
318 aa  136  6.0000000000000005e-31  Burkholderia phytofirmans PsJN  Bacteria  normal  normal 
 
 
-
 
NC_013517  Sterm_1525  binding-protein-dependent transport systems inner membrane component  32.33 
 
 
292 aa  135  7.000000000000001e-31  Sebaldella termitidis ATCC 33386  Bacteria  normal  n/a   
 
 
-
 
NC_011894  Mnod_1414  binding-protein-dependent transport systems inner membrane component  31.4 
 
 
314 aa  135  8e-31  Methylobacterium nodulans ORS 2060  Bacteria  normal  n/a   
 
 
-
 
NC_012669  Bcav_2875  binding-protein-dependent transport systems inner membrane component  34.04 
 
 
318 aa  134  9.999999999999999e-31  Beutenbergia cavernae DSM 12333  Bacteria  normal  0.847542  normal  0.0272583 
 
 
-
 
NC_010552  BamMC406_4681  binding-protein-dependent transport systems inner membrane component  34.72 
 
 
289 aa  134  1.9999999999999998e-30  Burkholderia ambifaria MC40-6  Bacteria  normal  0.205446  normal 
 
 
-
 
NC_013739  Cwoe_0172  binding-protein-dependent transport systems inner membrane component  32.85 
 
 
324 aa  134  1.9999999999999998e-30  Conexibacter woesei DSM 14684  Bacteria  normal  normal 
 
 
-
 
NC_008726  Mvan_2623  binding-protein-dependent transport systems inner membrane component  32.81 
 
 
287 aa  133  3e-30  Mycobacterium vanbaalenii PYR-1  Bacteria  normal  normal 
 
 
-
 
NC_011886  Achl_2900  binding-protein-dependent transport systems inner membrane component  33.11 
 
 
295 aa  133  3.9999999999999996e-30  Arthrobacter chlorophenolicus A6  Bacteria  n/a    normal 
 
 
-
 
NC_013526  Tter_2217  binding-protein-dependent transport systems inner membrane component  31.35 
 
 
305 aa  132  6e-30  Thermobaculum terrenum ATCC BAA-798  Bacteria  normal  n/a   
 
 
-
 
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