95 homologs were found in PanDaTox collection
for query gene Tfu_0522 on replicon NC_007333
Organism: Thermobifida fusca YX



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Replicon accession

Locus tag

Product

% identity

Alignment on query gene

Alignment on homolog gene

Length

Bit score

E-value

Organism name

Kingdom

Plasmid clonability

Plasmid unclonability p-value

Fosmid clonability

Fosmid unclonability p-value

Coverage Plot

PanDaTox homologs

Experimental validation
NC_007333  Tfu_0522  hypothetical protein  100 
 
 
108 aa  214  4e-55  Thermobifida fusca YX  Bacteria  normal  0.55986  n/a   
 
 
-
 
NC_014210  Ndas_3823  Methylated-DNA-(protein)-cysteine S-methyltransferase DNA binding protein  72.16 
 
 
108 aa  146  1.0000000000000001e-34  Nocardiopsis dassonvillei subsp. dassonvillei DSM 43111  Bacteria  normal  0.13049  normal  0.115407 
 
 
-
 
NC_013510  Tcur_3755  Methylated-DNA-(protein)-cysteine S- methyltransferase DNA binding protein  70.1 
 
 
102 aa  140  6e-33  Thermomonospora curvata DSM 43183  Bacteria  normal  0.15166  n/a   
 
 
-
 
NC_013595  Sros_8379  methylated DNA-protein cysteine methyltransferase-like protein  69.79 
 
 
106 aa  129  1.0000000000000001e-29  Streptosporangium roseum DSM 43021  Bacteria  normal  normal 
 
 
-
 
NC_013947  Snas_4277  Methylated-DNA-(protein)-cysteine S- methyltransferase DNA binding protein  64.95 
 
 
104 aa  128  3e-29  Stackebrandtia nassauensis DSM 44728  Bacteria  normal  0.421215  normal 
 
 
-
 
NC_013131  Caci_7813  Methylated-DNA-(protein)-cysteine S-methyltransferase DNA binding  64.84 
 
 
123 aa  119  9.999999999999999e-27  Catenulispora acidiphila DSM 44928  Bacteria  normal  normal 
 
 
-
 
NC_014165  Tbis_2976  methylated-DNA-(protein)-cysteine S- methyltransferase DNA binding protein  62 
 
 
125 aa  119  1.9999999999999998e-26  Thermobispora bispora DSM 43833  Bacteria  normal  normal  0.137628 
 
 
-
 
NC_008541  Arth_2758  methylated-DNA-(protein)-cysteine S-methyltransferase  51.96 
 
 
138 aa  101  3e-21  Arthrobacter sp. FB24  Bacteria  normal  0.0270311  n/a   
 
 
-
 
NC_011886  Achl_2480  Methylated-DNA-(protein)-cysteine S-methyltransferase DNA binding  50 
 
 
138 aa  100  8e-21  Arthrobacter chlorophenolicus A6  Bacteria  n/a    hitchhiker  0.0000000000803945 
 
 
-
 
NC_009664  Krad_1169  Methylated-DNA-(protein)-cysteine S-methyltransferase DNA binding  52 
 
 
162 aa  96.7  1e-19  Kineococcus radiotolerans SRS30216  Bacteria  normal  0.0168514  normal  0.300574 
 
 
-
 
NC_007777  Francci3_3802  methylated-DNA-(protein)-cysteine S-methyltransferase  52.53 
 
 
128 aa  95.1  3e-19  Frankia sp. CcI3  Bacteria  normal  0.298794  normal  0.741402 
 
 
-
 
NC_013521  Sked_24480  predicted methylated DNA-protein cysteine methyltransferase  58.06 
 
 
101 aa  92.4  2e-18  Sanguibacter keddieii DSM 10542  Bacteria  normal  normal  0.905071 
 
 
-
 
NC_008699  Noca_1481  methylated-DNA-(protein)-cysteine S-methyltransferase  52.38 
 
 
113 aa  91.3  4e-18  Nocardioides sp. JS614  Bacteria  decreased coverage  0.0000777093  n/a   
 
 
-
 
NC_009953  Sare_0458  methylated-DNA-(protein)-cysteine S-methyltransferase DNA binding  50.45 
 
 
111 aa  87  7e-17  Salinispora arenicola CNS-205  Bacteria  normal  hitchhiker  0.0000961012 
 
 
-
 
NC_009921  Franean1_0923  methylated-DNA-(protein)-cysteine S-methyltransferase DNA binding  51.02 
 
 
139 aa  84.3  5e-16  Frankia sp. EAN1pec  Bacteria  normal  0.211167  normal  0.90669 
 
 
-
 
NC_009380  Strop_0387  methylated-DNA-(protein)-cysteine S-methyltransferase  52.17 
 
 
95 aa  79.3  0.00000000000001  Salinispora tropica CNB-440  Bacteria  normal  0.687603  normal 
 
 
-
 
NC_013757  Gobs_4245  Methylated-DNA-(protein)-cysteine S- methyltransferase DNA binding protein  52.17 
 
 
129 aa  76.6  0.00000000000009  Geodermatophilus obscurus DSM 43160  Bacteria  normal  0.138963  n/a   
 
 
-
 
NC_013172  Bfae_26580  predicted methylated DNA-protein cysteine methyltransferase  50 
 
 
128 aa  74.3  0.0000000000006  Brachybacterium faecium DSM 4810  Bacteria  normal  0.618403  n/a   
 
 
-
 
NC_013530  Xcel_1107  Methylated-DNA-(protein)-cysteine S- methyltransferase DNA binding protein  39.32 
 
 
140 aa  73.6  0.0000000000009  Xylanimonas cellulosilytica DSM 15894  Bacteria  normal  0.542192  n/a   
 
 
-
 
NC_014151  Cfla_1378  Methylated-DNA-(protein)-cysteine S- methyltransferase DNA binding protein  46.36 
 
 
118 aa  73.2  0.000000000001  Cellulomonas flavigena DSM 20109  Bacteria  normal  hitchhiker  0.00436216 
 
 
-
 
NC_013159  Svir_06790  predicted methylated DNA-protein cysteine methyltransferase  45.26 
 
 
96 aa  67.8  0.00000000005  Saccharomonospora viridis DSM 43017  Bacteria  normal  normal  0.825443 
 
 
-
 
NC_013172  Bfae_04740  predicted methylated DNA-protein cysteine methyltransferase  45.45 
 
 
97 aa  65.5  0.0000000002  Brachybacterium faecium DSM 4810  Bacteria  normal  n/a   
 
 
-
 
NC_012669  Bcav_2615  hypothetical protein  41.9 
 
 
113 aa  65.1  0.0000000003  Beutenbergia cavernae DSM 12333  Bacteria  normal  0.0349096  normal 
 
 
-
 
NC_013235  Namu_2142  Methylated-DNA-(protein)-cysteine S- methyltransferase DNA binding  39.58 
 
 
95 aa  63.2  0.000000001  Nakamurella multipartita DSM 44233  Bacteria  hitchhiker  0.00259663  hitchhiker  0.00454826 
 
 
-
 
NC_009565  TBFG_13228  DNA-methyltransferase  37.11 
 
 
101 aa  60.1  0.000000009  Mycobacterium tuberculosis F11  Bacteria  normal  normal 
 
 
-
 
NC_013739  Cwoe_1040  Methylated-DNA-(protein)-cysteine S- methyltransferase DNA binding protein  45.36 
 
 
91 aa  59.3  0.00000002  Conexibacter woesei DSM 14684  Bacteria  normal  normal  0.42897 
 
 
-
 
NC_013441  Gbro_3503  Methylated-DNA-(protein)-cysteine S- methyltransferase DNA binding protein  38.3 
 
 
102 aa  58.2  0.00000003  Gordonia bronchialis DSM 43247  Bacteria  normal  0.0575204  n/a   
 
 
-
 
NC_009708  YpsIP31758_3074  methylated-DNA-[protein]-cysteine S-methyltransferase family protein  37.62 
 
 
187 aa  57.4  0.00000006  Yersinia pseudotuberculosis IP 31758  Bacteria  hitchhiker  0.0000014332  n/a   
 
 
-
 
NC_014158  Tpau_1129  Methylated-DNA-(protein)-cysteine S- methyltransferase DNA binding protein  41.56 
 
 
96 aa  57  0.00000008  Tsukamurella paurometabola DSM 20162  Bacteria  normal  0.845945  n/a   
 
 
-
 
NC_012803  Mlut_15110  predicted methylated DNA-protein cysteine methyltransferase  48.15 
 
 
136 aa  57  0.00000009  Micrococcus luteus NCTC 2665  Bacteria  normal  n/a   
 
 
-
 
NC_010465  YPK_3215  methylated-DNA--protein-cysteine methyltransferase  36.63 
 
 
187 aa  56.6  0.0000001  Yersinia pseudotuberculosis YPIII  Bacteria  normal  0.108204  n/a   
 
 
-
 
NC_010159  YpAngola_A3036  methylated-DNA-[protein]-cysteine S-methyltransferase family protein  37.62 
 
 
129 aa  56.6  0.0000001  Yersinia pestis Angola  Bacteria  normal  0.157696  normal  0.104694 
 
 
-
 
NC_008726  Mvan_1799  methylated-DNA-(protein)-cysteine S-methyltransferase  38.46 
 
 
98 aa  55.5  0.0000002  Mycobacterium vanbaalenii PYR-1  Bacteria  normal  normal  0.135987 
 
 
-
 
NC_008705  Mkms_1419  methylated-DNA-(protein)-cysteine S-methyltransferase  39.74 
 
 
99 aa  55.1  0.0000003  Mycobacterium sp. KMS  Bacteria  normal  0.304369  normal 
 
 
-
 
NC_008146  Mmcs_1401  methylated-DNA-(protein)-cysteine S-methyltransferase  39.74 
 
 
99 aa  55.1  0.0000003  Mycobacterium sp. MCS  Bacteria  normal  n/a   
 
 
-
 
NC_009077  Mjls_1455  methylated-DNA-(protein)-cysteine S-methyltransferase  38.46 
 
 
99 aa  53.1  0.000001  Mycobacterium sp. JLS  Bacteria  normal  0.777605  normal 
 
 
-
 
NC_011830  Dhaf_4686  methylated-DNA/protein-cysteine methyltransferase  38.14 
 
 
97 aa  52.4  0.000002  Desulfitobacterium hafniense DCB-2  Bacteria  decreased coverage  9.71623e-16  n/a   
 
 
-
 
NC_012917  PC1_1064  methylated-DNA/protein-cysteine methyltransferase  34.69 
 
 
106 aa  52.4  0.000002  Pectobacterium carotovorum subsp. carotovorum PC1  Bacteria  normal  n/a   
 
 
-
 
NC_013421  Pecwa_3247  methylated-DNA/protein-cysteine methyltransferase  33.67 
 
 
106 aa  52  0.000003  Pectobacterium wasabiae WPP163  Bacteria  normal  n/a   
 
 
-
 
NC_013169  Ksed_03160  predicted methylated DNA-protein cysteine methyltransferase  36.59 
 
 
113 aa  51.2  0.000004  Kytococcus sedentarius DSM 20547  Bacteria  normal  normal 
 
 
-
 
NC_008025  Dgeo_2101  methylated-DNA-(protein)-cysteine S-methyltransferase  46.03 
 
 
124 aa  51.6  0.000004  Deinococcus geothermalis DSM 11300  Bacteria  normal  0.440503  normal 
 
 
-
 
NC_009338  Mflv_4668  methylated-DNA-(protein)-cysteine S-methyltransferase  37.23 
 
 
98 aa  51.2  0.000005  Mycobacterium gilvum PYR-GCK  Bacteria  normal  0.199214  normal  0.161255 
 
 
-
 
NC_012912  Dd1591_3053  methylated-DNA/protein-cysteine methyltransferase  34.31 
 
 
108 aa  50.8  0.000005  Dickeya zeae Ech1591  Bacteria  normal  0.0335584  n/a   
 
 
-
 
NC_009436  Ent638_0922  methylated-DNA-(protein)-cysteine S-methyltransferase  37.14 
 
 
103 aa  49.7  0.00001  Enterobacter sp. 638  Bacteria  normal  0.259635  normal 
 
 
-
 
NC_013093  Amir_0853  Methylated-DNA-(protein)-cysteine S- methyltransferase DNA binding  32.29 
 
 
111 aa  49.3  0.00002  Actinosynnema mirum DSM 43827  Bacteria  normal  0.517473  n/a   
 
 
-
 
NC_013170  Ccur_10170  O-6-methylguanine DNA methyltransferase  39.42 
 
 
126 aa  49.7  0.00002  Cryptobacterium curtum DSM 15641  Bacteria  normal  0.0441838  normal  0.0108234 
 
 
-
 
NC_008527  LACR_1985  hypothetical protein  34.48 
 
 
101 aa  49.3  0.00002  Lactococcus lactis subsp. cremoris SK11  Bacteria  normal  n/a   
 
 
-
 
NC_012892  B21_00410  hypothetical protein  35.85 
 
 
129 aa  48.5  0.00003  Escherichia coli BL21  Bacteria  normal  n/a   
 
 
-
 
NC_010498  EcSMS35_0498  methylated-DNA-[protein]-cysteine S-methyltransferase  35.85 
 
 
103 aa  48.1  0.00003  Escherichia coli SMS-3-5  Bacteria  normal  normal 
 
 
-
 
NC_011353  ECH74115_0544  methylated-DNA-[protein]-cysteine S-methyltransferase  35.85 
 
 
103 aa  48.1  0.00003  Escherichia coli O157:H7 str. EC4115  Bacteria  normal  0.687414  normal 
 
 
-
 
NC_011205  SeD_A0510  methylated-DNA-[protein]-cysteine S-methyltransferase  37.14 
 
 
129 aa  48.5  0.00003  Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853  Bacteria  normal  0.326177  normal 
 
 
-
 
NC_011080  SNSL254_A0516  methylated-DNA-[protein]-cysteine S-methyltransferase  37.14 
 
 
129 aa  48.5  0.00003  Salmonella enterica subsp. enterica serovar Newport str. SL254  Bacteria  normal  0.0810186  normal 
 
 
-
 
CP001637  EcDH1_3155  methylated-DNA/protein-cysteine methyltransferase  35.85 
 
 
103 aa  48.1  0.00003  Escherichia coli DH1  Bacteria  normal  0.761097  n/a   
 
 
-
 
NC_008740  Maqu_2034  methylated-DNA--protein-cysteine methyltransferase  36.08 
 
 
107 aa  48.5  0.00003  Marinobacter aquaeolei VT8  Bacteria  normal  0.147166  n/a   
 
 
-
 
NC_009800  EcHS_A0531  methylated-DNA-[protein]-cysteine S-methyltransferase  35.85 
 
 
129 aa  48.5  0.00003  Escherichia coli HS  Bacteria  normal  n/a   
 
 
-
 
NC_009801  EcE24377A_0490  methylated-DNA-[protein]-cysteine S-methyltransferase  35.85 
 
 
129 aa  48.5  0.00003  Escherichia coli E24377A  Bacteria  normal  n/a   
 
 
-
 
NC_010468  EcolC_3161  methylated-DNA--protein-cysteine methyltransferase  35.85 
 
 
103 aa  48.1  0.00003  Escherichia coli ATCC 8739  Bacteria  normal  normal 
 
 
-
 
NC_010658  SbBS512_E0384  methylated-DNA-[protein]-cysteine S-methyltransferase  35.85 
 
 
129 aa  48.5  0.00003  Shigella boydii CDC 3083-94  Bacteria  normal  n/a   
 
 
-
 
CP001509  ECD_00406  predicted methyltransferase  35.85 
 
 
129 aa  48.5  0.00003  Escherichia coli BL21(DE3)  Bacteria  normal  n/a   
 
 
-
 
NC_011083  SeHA_C0569  methylated-DNA-[protein]-cysteine S-methyltransferase  37.14 
 
 
129 aa  48.1  0.00004  Salmonella enterica subsp. enterica serovar Heidelberg str. SL476  Bacteria  normal  normal 
 
 
-
 
NC_011094  SeSA_A0526  methylated-DNA-[protein]-cysteine S-methyltransferase  37.14 
 
 
129 aa  48.1  0.00004  Salmonella enterica subsp. enterica serovar Schwarzengrund str. CVM19633  Bacteria  normal  0.0387956  normal 
 
 
-
 
NC_011149  SeAg_B0507  methylated-DNA-[protein]-cysteine S-methyltransferase  36.19 
 
 
129 aa  47  0.00008  Salmonella enterica subsp. enterica serovar Agona str. SL483  Bacteria  hitchhiker  0.0000241332  n/a   
 
 
-
 
NC_008255  CHU_1048  methylated DNA-protein cysteine methyltransferase  31.68 
 
 
112 aa  46.2  0.0001  Cytophaga hutchinsonii ATCC 33406  Bacteria  normal  0.106489  normal  0.387859 
 
 
-
 
NC_012880  Dd703_1007  methylated-DNA/protein-cysteine methyltransferase  32.67 
 
 
105 aa  46.6  0.0001  Dickeya dadantii Ech703  Bacteria  normal  0.717266  n/a   
 
 
-
 
NC_009832  Spro_1113  methylated-DNA--protein-cysteine methyltransferase  43.08 
 
 
104 aa  47  0.0001  Serratia proteamaculans 568  Bacteria  decreased coverage  0.000000514305  hitchhiker  0.00000145406 
 
 
-
 
NC_013501  Rmar_2504  methylated-DNA/protein- cysteinemethyltransferase  47.46 
 
 
137 aa  47  0.0001  Rhodothermus marinus DSM 4252  Bacteria  normal  n/a   
 
 
-
 
NC_011662  Tmz1t_0061  methylated-DNA/protein-cysteine methyltransferase  45.76 
 
 
292 aa  45.4  0.0002  Thauera sp. MZ1T  Bacteria  normal  n/a   
 
 
-
 
NC_011884  Cyan7425_4389  Methylated-DNA-(protein)-cysteine S-methyltransferase DNA binding  35.35 
 
 
133 aa  45.1  0.0003  Cyanothece sp. PCC 7425  Bacteria  normal  0.315858  normal  0.794502 
 
 
-
 
NC_009783  VIBHAR_01452  hypothetical protein  28.28 
 
 
100 aa  45.4  0.0003  Vibrio harveyi ATCC BAA-1116  Bacteria  n/a    n/a   
 
 
-
 
NC_013203  Apar_0739  methylated-DNA/protein- cysteinemethyltransferase  33.65 
 
 
103 aa  44.3  0.0005  Atopobium parvulum DSM 20469  Bacteria  normal  normal 
 
 
-
 
NC_009675  Anae109_0922  methylated-DNA-(protein)-cysteine S-methyltransferase DNA binding  40.26 
 
 
139 aa  43.9  0.0007  Anaeromyxobacter sp. Fw109-5  Bacteria  normal  normal  0.266437 
 
 
-
 
NC_013456  VEA_004012  predicted methylated DNA-protein cysteine methyltransferase  27.27 
 
 
100 aa  43.9  0.0007  Vibrio sp. Ex25  Bacteria  normal  n/a   
 
 
-
 
NC_010830  Aasi_0280  hypothetical protein  38.1 
 
 
113 aa  43.5  0.0009  Candidatus Amoebophilus asiaticus 5a2  Bacteria  n/a    normal  0.584124 
 
 
-
 
NC_009654  Mmwyl1_2034  methylated-DNA-(protein)-cysteine S-methyltransferase DNA binding  35 
 
 
106 aa  43.1  0.001  Marinomonas sp. MWYL1  Bacteria  normal  0.778533  normal 
 
 
-
 
NC_008321  Shewmr4_2143  methylated-DNA-(protein)-cysteine S-methyltransferase  33.33 
 
 
152 aa  42.7  0.002  Shewanella sp. MR-4  Bacteria  normal  normal  0.841587 
 
 
-
 
NC_008322  Shewmr7_2220  methylated-DNA-(protein)-cysteine S-methyltransferase  33.33 
 
 
152 aa  42.7  0.002  Shewanella sp. MR-7  Bacteria  normal  0.0545564  normal  0.305905 
 
 
-
 
NC_009253  Dred_2924  methylated-DNA--protein-cysteine methyltransferase  41.38 
 
 
176 aa  42.4  0.002  Desulfotomaculum reducens MI-1  Bacteria  hitchhiker  0.000844149  n/a   
 
 
-
 
NC_008577  Shewana3_2320  methylated-DNA-(protein)-cysteine S-methyltransferase  33.33 
 
 
152 aa  42  0.003  Shewanella sp. ANA-3  Bacteria  decreased coverage  0.0000000931054  normal  0.649756 
 
 
-
 
NC_007510  Bcep18194_A4931  AraC family transcriptional regulator  39.34 
 
 
354 aa  42  0.003  Burkholderia sp. 383  Bacteria  normal  normal 
 
 
-
 
NC_008009  Acid345_1062  methylated-DNA-(protein)-cysteine S-methyltransferase  43.33 
 
 
123 aa  42  0.003  Candidatus Koribacter versatilis Ellin345  Bacteria  normal  0.0944604  normal  0.197926 
 
 
-
 
NC_009457  VC0395_A0583  MGMT family protein  33.87 
 
 
100 aa  42  0.003  Vibrio cholerae O395  Bacteria  hitchhiker  0.00000304888  n/a   
 
 
-
 
NC_013061  Phep_4217  Methylated-DNA-(protein)-cysteine S-methyltransferase DNA binding  39.06 
 
 
108 aa  41.2  0.004  Pedobacter heparinus DSM 2366  Bacteria  normal  0.920352  normal 
 
 
-
 
NC_013173  Dbac_1194  Methylated-DNA-(protein)-cysteine S- methyltransferase DNA binding  36.07 
 
 
123 aa  41.6  0.004  Desulfomicrobium baculatum DSM 4028  Bacteria  normal  0.134413  n/a   
 
 
-
 
NC_009523  RoseRS_3624  methylated-DNA-(protein)-cysteine S-methyltransferase  36 
 
 
104 aa  41.6  0.004  Roseiflexus sp. RS-1  Bacteria  normal  0.100308  normal 
 
 
-
 
NC_009656  PSPA7_3190  O6-methylguanine-DNA methyltransferase  38.89 
 
 
358 aa  41.2  0.004  Pseudomonas aeruginosa PA7  Bacteria  normal  n/a   
 
 
-
 
NC_003295  RSc2570  bifunctional methylated-DNA--protein-cysteine methyltransferase/O-6-methylguanine-DNA transcription regulator  39.34 
 
 
360 aa  41.2  0.005  Ralstonia solanacearum GMI1000  Bacteria  normal  normal 
 
 
-
 
NC_009901  Spea_2390  methylated-DNA-(protein)-cysteine S-methyltransferase DNA binding  34.34 
 
 
117 aa  41.2  0.005  Shewanella pealeana ATCC 700345  Bacteria  normal  n/a   
 
 
-
 
NC_013517  Sterm_2186  Methylated-DNA-(protein)-cysteine S- methyltransferase DNA binding protein  39.34 
 
 
113 aa  40.8  0.006  Sebaldella termitidis ATCC 33386  Bacteria  hitchhiker  0.00000000163972  n/a   
 
 
-
 
NC_009667  Oant_0476  methylated-DNA--protein-cysteine methyltransferase  39.34 
 
 
361 aa  40.4  0.007  Ochrobactrum anthropi ATCC 49188  Bacteria  normal  n/a   
 
 
-
 
NC_011894  Mnod_0501  transcriptional regulator, AraC family  35.48 
 
 
374 aa  40.4  0.008  Methylobacterium nodulans ORS 2060  Bacteria  normal  0.399439  n/a   
 
 
-
 
NC_008463  PA14_37190  O6-methylguanine-DNA methyltransferase  40.98 
 
 
358 aa  40.4  0.008  Pseudomonas aeruginosa UCBPP-PA14  Bacteria  normal  0.0320795  normal 
 
 
-
 
NC_003910  CPS_3583  putative methylated-DNA binding protein  28.71 
 
 
125 aa  40  0.009  Colwellia psychrerythraea 34H  Bacteria  normal  n/a   
 
 
-
 
NC_007519  Dde_3099  methylated-DNA--protein-cysteine methyltransferase  37.31 
 
 
203 aa  40  0.01  Desulfovibrio desulfuricans subsp. desulfuricans str. G20  Bacteria  normal  n/a   
 
 
-
 
NC_007974  Rmet_3765  bifunctional DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase  40.68 
 
 
354 aa  40  0.01  Cupriavidus metallidurans CH34  Bacteria  normal  normal 
 
 
-
 
NC_010717  PXO_01916  methylated-DNA-protein-cysteine S-methyltransferase related protein  35.58 
 
 
135 aa  40  0.01  Xanthomonas oryzae pv. oryzae PXO99A  Bacteria  normal  n/a   
 
 
-
 
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