More than 300 homologs were found in PanDaTox collection
for query gene Dfer_5571 on replicon NC_013037
Organism: Dyadobacter fermentans DSM 18053



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Replicon accession

Locus tag

Product

% identity

Alignment on query gene

Alignment on homolog gene

Length

Bit score

E-value

Organism name

Kingdom

Plasmid clonability

Plasmid unclonability p-value

Fosmid clonability

Fosmid unclonability p-value

Coverage Plot

PanDaTox homologs

Experimental validation
NC_008752  Aave_2417  signal transduction histidine kinase with CheB and CheR activity  44.86 
 
 
1535 aa  716    Acidovorax citrulli AAC00-1  Bacteria  normal  0.117494  normal 
 
 
-
 
NC_007348  Reut_B4915  PAS  48.06 
 
 
1384 aa  772    Ralstonia eutropha JMP134  Bacteria  normal  n/a   
 
 
-
 
NC_008781  Pnap_1703  signal transduction histidine kinase with CheB and CheR activity  43.19 
 
 
1408 aa  718    Polaromonas naphthalenivorans CJ2  Bacteria  normal  0.433463  normal 
 
 
-
 
NC_013037  Dfer_5571  signal transduction histidine kinase with CheB and CheR activity  100 
 
 
1218 aa  2489    Dyadobacter fermentans DSM 18053  Bacteria  normal  0.273526  normal 
 
 
-
 
NC_010322  PputGB1_3018  signal transduction histidine kinase with CheB and CheR activity  42.14 
 
 
1371 aa  684    Pseudomonas putida GB-1  Bacteria  normal  normal  0.980757 
 
 
-
 
NC_009712  Mboo_0327  putative PAS/PAC sensor protein  39.82 
 
 
1008 aa  640    Candidatus Methanoregula boonei 6A8  Archaea  normal  normal 
 
 
-
 
NC_010086  Bmul_3193  signal transduction histidine kinase with CheB and CheR activity  42.11 
 
 
1380 aa  665    Burkholderia multivorans ATCC 17616  Bacteria  normal  normal  0.112764 
 
 
-
 
NC_007484  Noc_1601  CheB methylesterase, CheR methyltransferase, hybrid histidine kinase  39.61 
 
 
1222 aa  918    Nitrosococcus oceani ATCC 19707  Bacteria  normal  0.472526  n/a   
 
 
-
 
NC_011891  A2cp1_3377  signal transduction histidine kinase with CheB and CheR activity  38.9 
 
 
1242 aa  822    Anaeromyxobacter dehalogenans 2CP-1  Bacteria  normal  n/a   
 
 
-
 
NC_007760  Adeh_3183  signal transduction histidine kinase  38.9 
 
 
1279 aa  815    Anaeromyxobacter dehalogenans 2CP-C  Bacteria  normal  n/a   
 
 
-
 
NC_011145  AnaeK_0372  signal transduction histidine kinase with CheB and CheR activity  40.14 
 
 
1233 aa  847    Anaeromyxobacter sp. K  Bacteria  normal  n/a   
 
 
-
 
NC_010625  Bphy_6399  signal transduction histidine kinase with CheB and CheR activity  47.64 
 
 
1361 aa  806    Burkholderia phymatum STM815  Bacteria  normal  0.191888  normal 
 
 
-
 
NC_013037  Dfer_5176  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  65.9 
 
 
1404 aa  1116    Dyadobacter fermentans DSM 18053  Bacteria  normal  normal  0.190634 
 
 
-
 
NC_010580  Bind_3835  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  42.25 
 
 
1167 aa  672    Beijerinckia indica subsp. indica ATCC 9039  Bacteria  normal  normal 
 
 
-
 
NC_009621  Smed_5776  putative PAS/PAC sensor protein  41.68 
 
 
1160 aa  685    Sinorhizobium medicae WSM419  Bacteria  normal  0.491611  normal  0.0137055 
 
 
-
 
NC_013132  Cpin_4422  signal transduction histidine kinase with CheB and CheR activity  61.64 
 
 
1274 aa  1088    Chitinophaga pinensis DSM 2588  Bacteria  normal  0.381764  normal 
 
 
-
 
NC_007517  Gmet_0780  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  40.85 
 
 
887 aa  632  1e-179  Geobacter metallireducens GS-15  Bacteria  normal  normal 
 
 
-
 
NC_010803  Clim_1600  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  39.63 
 
 
1000 aa  626  1e-178  Chlorobium limicola DSM 245  Bacteria  normal  n/a   
 
 
-
 
NC_007643  Rru_A1500  Signal transduction histidine kinase (STHK) with CheB and CheR activity  34.71 
 
 
1483 aa  629  1e-178  Rhodospirillum rubrum ATCC 11170  Bacteria  normal  n/a   
 
 
-
 
NC_013173  Dbac_2162  MCP methyltransferase/methylesterase, CheR/CheB  39.86 
 
 
879 aa  623  1e-177  Desulfomicrobium baculatum DSM 4028  Bacteria  normal  n/a   
 
 
-
 
NC_011060  Ppha_1653  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  38.55 
 
 
993 aa  619  1e-176  Pelodictyon phaeoclathratiforme BU-1  Bacteria  normal  n/a   
 
 
-
 
NC_009467  Acry_3169  signal transduction histidine kinase with CheB and CheR activity  34.04 
 
 
1445 aa  619  1e-175  Acidiphilium cryptum JF-5  Bacteria  normal  n/a   
 
 
-
 
NC_010508  Bcenmc03_1302  signal transduction histidine kinase with CheB and CheR activity  33.41 
 
 
1220 aa  614  9.999999999999999e-175  Burkholderia cenocepacia MC0-3  Bacteria  normal  normal  0.772751 
 
 
-
 
NC_007355  Mbar_A2183  hypothetical protein  39.01 
 
 
980 aa  611  1e-173  Methanosarcina barkeri str. Fusaro  Archaea  normal  normal  0.850093 
 
 
-
 
NC_010086  Bmul_4696  signal transduction histidine kinase with CheB and CheR activity  33.95 
 
 
1214 aa  610  1e-173  Burkholderia multivorans ATCC 17616  Bacteria  normal  normal 
 
 
-
 
NC_011060  Ppha_0229  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  39.58 
 
 
1138 aa  610  1e-173  Pelodictyon phaeoclathratiforme BU-1  Bacteria  normal  n/a   
 
 
-
 
NC_007298  Daro_2045  CheB methylesterase:MCP methyltransferase, CheR-type  39.41 
 
 
868 aa  604  1.0000000000000001e-171  Dechloromonas aromatica RCB  Bacteria  normal  normal 
 
 
-
 
NC_007908  Rfer_2355  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  39.84 
 
 
1008 aa  605  1.0000000000000001e-171  Rhodoferax ferrireducens T118  Bacteria  normal  0.71741  n/a   
 
 
-
 
NC_008639  Cpha266_1788  putative PAS/PAC sensor protein  38.8 
 
 
998 aa  603  1.0000000000000001e-171  Chlorobium phaeobacteroides DSM 266  Bacteria  normal  n/a   
 
 
-
 
NC_009620  Smed_4278  putative PAS/PAC sensor protein  36.74 
 
 
1027 aa  602  1e-170  Sinorhizobium medicae WSM419  Bacteria  normal  normal 
 
 
-
 
NC_007908  Rfer_3013  MCP methyltransferase, CheR-type  40.12 
 
 
877 aa  601  1e-170  Rhodoferax ferrireducens T118  Bacteria  normal  0.404945  n/a   
 
 
-
 
NC_007514  Cag_0563  putative PAS/PAC sensor protein  38.17 
 
 
1035 aa  584  1.0000000000000001e-165  Chlorobium chlorochromatii CaD3  Bacteria  normal  n/a   
 
 
-
 
NC_011832  Mpal_2460  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  38.68 
 
 
971 aa  584  1.0000000000000001e-165  Methanosphaerula palustris E1-9c  Archaea  normal  normal 
 
 
-
 
NC_013205  Aaci_0673  signal transduction histidine kinase with CheB and CheR activity  37.62 
 
 
1215 aa  578  1.0000000000000001e-163  Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446  Bacteria  normal  n/a   
 
 
-
 
NC_010511  M446_6211  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  38.51 
 
 
1061 aa  573  1.0000000000000001e-162  Methylobacterium sp. 4-46  Bacteria  normal  0.820077  normal  0.703655 
 
 
-
 
NC_007961  Nham_4605  MCP methyltransferase, CheR-type  38.58 
 
 
1092 aa  572  1e-161  Nitrobacter hamburgensis X14  Bacteria  normal  0.315944  n/a   
 
 
-
 
NC_011894  Mnod_3755  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  37.2 
 
 
1063 aa  567  1e-160  Methylobacterium nodulans ORS 2060  Bacteria  normal  n/a   
 
 
-
 
NC_010725  Mpop_1297  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  40.12 
 
 
1168 aa  565  1.0000000000000001e-159  Methylobacterium populi BJ001  Bacteria  normal  normal 
 
 
-
 
NC_010581  Bind_0707  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  38.3 
 
 
1190 aa  564  1.0000000000000001e-159  Beijerinckia indica subsp. indica ATCC 9039  Bacteria  normal  normal 
 
 
-
 
NC_008709  Ping_2549  fused CheR-type MCP methyltransferase and PAS sensor protein  37.49 
 
 
1006 aa  565  1.0000000000000001e-159  Psychromonas ingrahamii 37  Bacteria  normal  normal 
 
 
-
 
NC_011004  Rpal_3739  MCP methyltransferase, CheR-type  37.91 
 
 
1045 aa  560  1e-158  Rhodopseudomonas palustris TIE-1  Bacteria  normal  n/a   
 
 
-
 
NC_014248  Aazo_2376  signal transduction histidine kinase with CheB and CheR activity  37.21 
 
 
1407 aa  553  1e-156  'Nostoc azollae' 0708  Bacteria  normal  n/a   
 
 
-
 
NC_007413  Ava_0314  Signal transduction histidine kinase (STHK) with CheB and CheR activity  37.37 
 
 
1399 aa  553  1e-156  Anabaena variabilis ATCC 29413  Bacteria  normal  normal 
 
 
-
 
NC_008709  Ping_3128  MCP methyltransferase, CheR-type  36.53 
 
 
840 aa  541  9.999999999999999e-153  Psychromonas ingrahamii 37  Bacteria  normal  normal 
 
 
-
 
NC_011368  Rleg2_5237  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  38.79 
 
 
1324 aa  540  9.999999999999999e-153  Rhizobium leguminosarum bv. trifolii WSM2304  Bacteria  normal  normal  0.395635 
 
 
-
 
NC_008789  Hhal_1716  MCP methyltransferase, CheR-type  37.59 
 
 
856 aa  541  9.999999999999999e-153  Halorhodospira halophila SL1  Bacteria  normal  0.92135  n/a   
 
 
-
 
NC_008709  Ping_1840  MCP methyltransferase, CheR-type  36.89 
 
 
840 aa  540  9.999999999999999e-153  Psychromonas ingrahamii 37  Bacteria  normal  0.59391  normal  0.603716 
 
 
-
 
NC_010510  Mrad2831_6264  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  39.56 
 
 
1163 aa  538  1e-151  Methylobacterium radiotolerans JCM 2831  Bacteria  normal  normal 
 
 
-
 
NC_008340  Mlg_1118  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  37.19 
 
 
852 aa  533  1e-149  Alkalilimnicola ehrlichii MLHE-1  Bacteria  normal  normal  0.742809 
 
 
-
 
NC_013173  Dbac_1410  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  35.33 
 
 
1120 aa  529  1e-148  Desulfomicrobium baculatum DSM 4028  Bacteria  normal  0.132886  n/a   
 
 
-
 
NC_007912  Sde_3046  hypothetical protein  35.77 
 
 
1010 aa  507  9.999999999999999e-143  Saccharophagus degradans 2-40  Bacteria  normal  hitchhiker  0.00164382 
 
 
-
 
NC_013440  Hoch_3250  signal transduction histidine kinase with CheB and CheR activity  33.99 
 
 
1348 aa  506  1e-141  Haliangium ochraceum DSM 14365  Bacteria  normal  0.534329  normal  0.0270765 
 
 
-
 
NC_013061  Phep_3913  PAS sensor protein  33.41 
 
 
1337 aa  494  9.999999999999999e-139  Pedobacter heparinus DSM 2366  Bacteria  normal  0.290527  normal  0.28306 
 
 
-
 
NC_014230  CA2559_12648  Signal Transduction Histidine Kinase (STHK) with CheB and CheRactivity  33.68 
 
 
1200 aa  487  1e-136  Croceibacter atlanticus HTCC2559  Bacteria  normal  n/a   
 
 
-
 
NC_013440  Hoch_3278  signal transduction histidine kinase with CheB and CheR activity  35.54 
 
 
2468 aa  487  1e-136  Haliangium ochraceum DSM 14365  Bacteria  normal  normal  0.0267066 
 
 
-
 
NC_008609  Ppro_3165  signal transduction histidine kinase with CheB and CheR activity  33.8 
 
 
1306 aa  488  1e-136  Pelobacter propionicus DSM 2379  Bacteria  normal  n/a   
 
 
-
 
NC_013132  Cpin_7206  signal transduction histidine kinase with CheB and CheR activity  32.47 
 
 
1499 aa  483  1e-135  Chitinophaga pinensis DSM 2588  Bacteria  normal  normal 
 
 
-
 
NC_010505  Mrad2831_4430  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  36.89 
 
 
1158 aa  486  1e-135  Methylobacterium radiotolerans JCM 2831  Bacteria  normal  normal 
 
 
-
 
NC_010725  Mpop_0310  MCP methyltransferase, CheR-type  35.31 
 
 
1149 aa  480  1e-134  Methylobacterium populi BJ001  Bacteria  normal  0.512157  normal 
 
 
-
 
NC_010505  Mrad2831_5709  MCP methyltransferase, CheR-type  34.56 
 
 
853 aa  483  1e-134  Methylobacterium radiotolerans JCM 2831  Bacteria  normal  normal  0.729466 
 
 
-
 
NC_009049  Rsph17029_0905  putative PAS/PAC sensor protein  34.88 
 
 
1170 aa  477  1e-133  Rhodobacter sphaeroides ATCC 17029  Bacteria  normal  normal  0.968727 
 
 
-
 
NC_007493  RSP_2229  methylesterase CheB/methylase CheR  34.88 
 
 
1170 aa  477  1e-133  Rhodobacter sphaeroides 2.4.1  Bacteria  normal  0.840236  n/a   
 
 
-
 
NC_007953  Bxe_C0521  multi sensor hybrid histidine kinase  35.34 
 
 
1344 aa  472  1.0000000000000001e-131  Burkholderia xenovorans LB400  Bacteria  normal  normal 
 
 
-
 
NC_009428  Rsph17025_2264  putative PAS/PAC sensor protein  34.38 
 
 
1165 aa  468  9.999999999999999e-131  Rhodobacter sphaeroides ATCC 17025  Bacteria  normal  0.620072  normal  0.0600346 
 
 
-
 
NC_007955  Mbur_0399  MCP methyltransferase, CheR-type  32.37 
 
 
820 aa  467  9.999999999999999e-131  Methanococcoides burtonii DSM 6242  Archaea  normal  n/a   
 
 
-
 
NC_007952  Bxe_B1520  signal transduction histidine kinase (STHK) with CheB and CheR activity  36.36 
 
 
1303 aa  462  9.999999999999999e-129  Burkholderia xenovorans LB400  Bacteria  normal  0.124967  normal 
 
 
-
 
NC_013730  Slin_3102  signal transduction histidine kinase with CheB and CheR activity  36.22 
 
 
1418 aa  460  9.999999999999999e-129  Spirosoma linguale DSM 74  Bacteria  normal  normal 
 
 
-
 
NC_013440  Hoch_4639  signal transduction histidine kinase with CheB and CheR activity  34.6 
 
 
1453 aa  462  9.999999999999999e-129  Haliangium ochraceum DSM 14365  Bacteria  normal  0.432269  normal 
 
 
-
 
NC_013440  Hoch_1404  signal transduction histidine kinase with CheB and CheR activity  33.64 
 
 
1759 aa  442  9.999999999999999e-123  Haliangium ochraceum DSM 14365  Bacteria  normal  normal 
 
 
-
 
NC_008255  CHU_1237  chemotaxis response regulator (methyltransferase)  32.07 
 
 
1618 aa  441  9.999999999999999e-123  Cytophaga hutchinsonii ATCC 33406  Bacteria  normal  normal 
 
 
-
 
NC_008751  Dvul_2487  signal transduction histidine kinase with CheB and CheR activity  34.26 
 
 
1535 aa  433  1e-120  Desulfovibrio vulgaris DP4  Bacteria  normal  0.162467  normal  0.455724 
 
 
-
 
NC_013440  Hoch_5252  signal transduction histidine kinase with CheB and CheR activity  33.26 
 
 
1698 aa  435  1e-120  Haliangium ochraceum DSM 14365  Bacteria  normal  normal 
 
 
-
 
NC_002977  MCA1246  methyltransferase CheR, putative  34.96 
 
 
1378 aa  421  1e-116  Methylococcus capsulatus str. Bath  Bacteria  normal  0.271052  n/a   
 
 
-
 
NC_008789  Hhal_0370  protein-glutamate O-methyltransferase  38.88 
 
 
639 aa  392  1e-107  Halorhodospira halophila SL1  Bacteria  normal  0.181962  n/a   
 
 
-
 
NC_008044  TM1040_1028  signal transduction histidine kinase with CheB and CheR activity  30.87 
 
 
1248 aa  387  1e-106  Ruegeria sp. TM1040  Bacteria  normal  normal  0.507304 
 
 
-
 
NC_008044  TM1040_0460  MCP methyltransferase, CheR-type  31.72 
 
 
837 aa  382  1e-104  Ruegeria sp. TM1040  Bacteria  normal  0.604812  normal 
 
 
-
 
NC_009622  Smed_6341  putative PAS/PAC sensor protein  38.72 
 
 
629 aa  377  1e-103  Sinorhizobium medicae WSM419  Bacteria  normal  0.433344  normal  0.230755 
 
 
-
 
NC_013037  Dfer_2246  multi-sensor hybrid histidine kinase  46.59 
 
 
1084 aa  372  1e-101  Dyadobacter fermentans DSM 18053  Bacteria  normal  normal  0.692403 
 
 
-
 
NC_013730  Slin_4198  PAS/PAC sensor hybrid histidine kinase  50.91 
 
 
1185 aa  372  1e-101  Spirosoma linguale DSM 74  Bacteria  normal  normal  0.996958 
 
 
-
 
NC_008576  Mmc1_3390  MCP methyltransferase, CheR-type  35.65 
 
 
617 aa  367  1e-100  Magnetococcus sp. MC-1  Bacteria  normal  normal 
 
 
-
 
NC_010002  Daci_4352  multi-sensor hybrid histidine kinase  46.19 
 
 
1287 aa  364  5.0000000000000005e-99  Delftia acidovorans SPH-1  Bacteria  normal  normal  0.06083 
 
 
-
 
NC_007802  Jann_2564  MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor  31.52 
 
 
1089 aa  363  8e-99  Jannaschia sp. CCS1  Bacteria  normal  normal 
 
 
-
 
NC_007348  Reut_B5191  CheB methylesterase:MCP methyltransferase, CheR-type  30.42 
 
 
813 aa  362  3e-98  Ralstonia eutropha JMP134  Bacteria  normal  n/a   
 
 
-
 
NC_010623  Bphy_5175  MCP methyltransferase, CheR-type  30.63 
 
 
823 aa  357  1e-96  Burkholderia phymatum STM815  Bacteria  normal  normal  0.367936 
 
 
-
 
NC_008044  TM1040_2227  MCP methyltransferase, CheR-type  31.4 
 
 
824 aa  354  4e-96  Ruegeria sp. TM1040  Bacteria  normal  normal 
 
 
-
 
NC_013730  Slin_1989  MCP methyltransferase, CheR-type with PAS/PAC sensor  36.75 
 
 
1110 aa  354  5e-96  Spirosoma linguale DSM 74  Bacteria  normal  0.175442  normal  0.013335 
 
 
-
 
NC_007964  Nham_3990  MCP methyltransferase, CheR-type  41.75 
 
 
432 aa  338  5e-91  Nitrobacter hamburgensis X14  Bacteria  normal  n/a   
 
 
-
 
NC_013440  Hoch_3141  PAS/PAC sensor hybrid histidine kinase  44.7 
 
 
1406 aa  331  6e-89  Haliangium ochraceum DSM 14365  Bacteria  normal  0.367834  normal  0.195519 
 
 
-
 
NC_010814  Glov_2975  diguanylate cyclase with PAS/PAC sensor  33.5 
 
 
1084 aa  328  4.0000000000000003e-88  Geobacter lovleyi SZ  Bacteria  normal  n/a   
 
 
-
 
NC_007614  Nmul_A2207  multi-sensor hybrid histidine kinase  44.85 
 
 
866 aa  328  5e-88  Nitrosospira multiformis ATCC 25196  Bacteria  normal  n/a   
 
 
-
 
NC_007484  Noc_1400  PAS sensor signal transduction histidine kinase  36.86 
 
 
938 aa  324  5e-87  Nitrosococcus oceani ATCC 19707  Bacteria  normal  n/a   
 
 
-
 
NC_007614  Nmul_A1490  PAS/PAC sensor hybrid histidine kinase  44.04 
 
 
835 aa  324  6e-87  Nitrosospira multiformis ATCC 25196  Bacteria  normal  n/a   
 
 
-
 
NC_007348  Reut_B4539  response regulator receiver:ATP-binding region, ATPase-like:histidine kinase A, N-terminal  45.45 
 
 
1152 aa  323  9.999999999999999e-87  Ralstonia eutropha JMP134  Bacteria  normal  0.329946  n/a   
 
 
-
 
NC_008228  Patl_1295  multi-sensor hybrid histidine kinase  40.83 
 
 
1223 aa  321  5e-86  Pseudoalteromonas atlantica T6c  Bacteria  normal  0.0981526  n/a   
 
 
-
 
NC_010717  PXO_03023  methyltransferase  34.54 
 
 
604 aa  319  2e-85  Xanthomonas oryzae pv. oryzae PXO99A  Bacteria  normal  0.361036  n/a   
 
 
-
 
NC_009675  Anae109_0539  multi-sensor hybrid histidine kinase  46.88 
 
 
816 aa  319  2e-85  Anaeromyxobacter sp. Fw109-5  Bacteria  normal  0.146269  normal 
 
 
-
 
NC_007952  Bxe_B1311  PAS/PAC sensor hybrid histidine kinase  41.13 
 
 
776 aa  319  2e-85  Burkholderia xenovorans LB400  Bacteria  normal  normal  0.103963 
 
 
-
 
NC_010676  Bphyt_5296  PAS/PAC sensor hybrid histidine kinase  40.2 
 
 
776 aa  318  3e-85  Burkholderia phytofirmans PsJN  Bacteria  normal  normal 
 
 
-
 
NC_010814  Glov_0566  MCP methyltransferase, CheR-type with PAS/PAC sensor  32.26 
 
 
1193 aa  312  2e-83  Geobacter lovleyi SZ  Bacteria  normal  n/a   
 
 
-
 
NC_010622  Bphy_0134  histidine kinase  43.19 
 
 
547 aa  310  1.0000000000000001e-82  Burkholderia phymatum STM815  Bacteria  normal  normal 
 
 
-
 
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