More than 300 homologs were found in PanDaTox collection
for query gene Bmul_4315 on replicon NC_010086
Organism: Burkholderia multivorans ATCC 17616



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Replicon accession
Locus tag
Product
% identity
Alignment on query gene
Alignment on homolog gene
Length
Bit score

E-value
Organism name
Kingdom
Plasmid clonability
Plasmid unclonability p-value
Fosmid clonability
Fosmid unclonability p-value
Coverage Plot
PanDaTox homologs
Experimental validation
NC_010086  Bmul_4315  two component heavy metal response transcriptional regulator  100 
 
 		 232 aa  455  1e-127  Burkholderia multivorans ATCC 17616  Bacteria  normal  0.767231  normal  0.644171 
 
 
-
 
NC_010552  BamMC406_4186  two component heavy metal response transcriptional regulator  95.69 
 
 		 231 aa  414  9.999999999999999e-116  Burkholderia ambifaria MC40-6  Bacteria  normal  0.24384  normal  0.790942 
 
 
-
 
NC_010515  Bcenmc03_3227  two component heavy metal response transcriptional regulator  96.98 
 
 		 231 aa  417  9.999999999999999e-116  Burkholderia cenocepacia MC0-3  Bacteria  normal  0.303749  normal  0.0627816 
 
 
-
 
NC_007511  Bcep18194_B1729  two component heavy metal response transcriptional regulator  97.39 
 
 		 231 aa  414  9.999999999999999e-116  Burkholderia sp. 383  Bacteria  normal  1  normal  1 
 
 
-
 
NC_008061  Bcen_4076  two component heavy metal response transcriptional regulator  96.98 
 
 		 231 aa  417  9.999999999999999e-116  Burkholderia cenocepacia AU 1054  Bacteria  normal  0.0105833  n/a   
 
 
-
 
NC_008391  Bamb_3708  two component heavy metal response transcriptional regulator  95.69 
 
 		 231 aa  414  9.999999999999999e-116  Burkholderia ambifaria AMMD  Bacteria  normal  1  normal  1 
 
 
-
 
NC_008543  Bcen2424_4290  two component heavy metal response transcriptional regulator  96.98 
 
 		 231 aa  417  9.999999999999999e-116  Burkholderia cenocepacia HI2424  Bacteria  normal  1  normal  1 
 
 
-
 
NC_010676  Bphyt_5873  two component heavy metal response transcriptional regulator, winged helix family  80 
 
 		 228 aa  366  1e-100  Burkholderia phytofirmans PsJN  Bacteria  normal  0.247228  normal  1 
 
 
-
 
NC_007952  Bxe_B2069  two component heavy metal response transcriptional regulator  80 
 
 		 228 aa  366  1e-100  Burkholderia xenovorans LB400  Bacteria  normal  0.0102482  normal  1 
 
 
-
 
NC_010625  Bphy_6519  two component heavy metal response transcriptional regulator  77.19 
 
 		 228 aa  354  5.999999999999999e-97  Burkholderia phymatum STM815  Bacteria  normal  1  normal  0.0435386 
 
 
-
 
NC_007492  Pfl01_4663  two component heavy metal response transcriptional regulator  74.32 
 
 		 225 aa  325  3e-88  Pseudomonas fluorescens Pf0-1  Bacteria  normal  0.180214  normal  0.242183 
 
 
-
 
NC_008786  Veis_0344  two component heavy metal response transcriptional regulator  67.69 
 
 		 231 aa  319  1.9999999999999998e-86  Verminephrobacter eiseniae EF01-2  Bacteria  normal  1  normal  1 
 
 
-
 
NC_004578  PSPTO_1307  DNA-binding heavy metal response regulator  70.67 
 
 		 225 aa  316  2e-85  Pseudomonas syringae pv. tomato str. DC3000  Bacteria  normal  0.150056  n/a   
 
 
-
 
NC_010322  PputGB1_0836  two component heavy metal response transcriptional regulator  71.62 
 
 		 224 aa  316  2e-85  Pseudomonas putida GB-1  Bacteria  normal  0.368612  normal  1 
 
 
-
 
NC_010501  PputW619_4391  two component heavy metal response transcriptional regulator  71.62 
 
 		 224 aa  315  3e-85  Pseudomonas putida W619  Bacteria  normal  1  normal  0.593256 
 
 
-
 
NC_007005  Psyr_1127  heavy metal response regulator  70.22 
 
 		 225 aa  315  4e-85  Pseudomonas syringae pv. syringae B728a  Bacteria  normal  0.294376  normal  0.216529 
 
 
-
 
NC_009656  PSPA7_3892  putative two-component response regulator  68.16 
 
 		 229 aa  303  2.0000000000000002e-81  Pseudomonas aeruginosa PA7  Bacteria  normal  1  n/a   
 
 
-
 
NC_008463  PA14_45880  putative two-component response regulator  67.71 
 
 		 229 aa  300  2e-80  Pseudomonas aeruginosa UCBPP-PA14  Bacteria  normal  1  normal  0.954426 
 
 
-
 
NC_010002  Daci_1091  two component heavy metal response transcriptional regulator  62.11 
 
 		 232 aa  279  2e-74  Delftia acidovorans SPH-1  Bacteria  normal  1  normal  1 
 
 
-
 
NC_010159  YpAngola_A2480  DNA-binding heavy metal response regulator  60.79 
 
 		 230 aa  276  2e-73  Yersinia pestis Angola  Bacteria  hitchhiker  0.00000187636  normal  0.518539 
 
 
-
 
NC_010465  YPK_2192  two component heavy metal response transcriptional regulator  60.18 
 
 		 230 aa  276  2e-73  Yersinia pseudotuberculosis YPIII  Bacteria  normal  1  n/a   
 
 
-
 
NC_008825  Mpe_A1629  two-component response regulator  61.95 
 
 		 228 aa  276  3e-73  Methylibium petroleiphilum PM1  Bacteria  normal  1  normal  0.128663 
 
 
-
 
NC_009708  YpsIP31758_2084  DNA-binding heavy metal response regulator  59.73 
 
 		 230 aa  275  5e-73  Yersinia pseudotuberculosis IP 31758  Bacteria  hitchhiker  0.000771382  n/a   
 
 
-
 
NC_012791  Vapar_3559  two component heavy metal response transcriptional regulator, winged helix family  60.36 
 
 		 225 aa  274  8e-73  Variovorax paradoxus S110  Bacteria  normal  0.710681  n/a   
 
 
-
 
NC_008391  Bamb_5186  two component heavy metal response transcriptional regulator  59.01 
 
 		 223 aa  269  2e-71  Burkholderia ambifaria AMMD  Bacteria  normal  1  normal  1 
 
 
-
 
NC_003296  RSp0489  response regulator for cobalt zinc cadmium resistance transcription regulator protein  59.09 
 
 		 224 aa  269  2.9999999999999997e-71  Ralstonia solanacearum GMI1000  Bacteria  normal  1  normal  1 
 
 
-
 
NC_007347  Reut_A1815  two component heavy metal response transcriptional regulator  59.91 
 
 		 224 aa  269  2.9999999999999997e-71  Ralstonia eutropha JMP134  Bacteria  normal  0.275759  n/a   
 
 
-
 
NC_010552  BamMC406_3349  two component heavy metal response transcriptional regulator  59.01 
 
 		 223 aa  269  2.9999999999999997e-71  Burkholderia ambifaria MC40-6  Bacteria  normal  1  normal  1 
 
 
-
 
NC_010676  Bphyt_5224  two component heavy metal response transcriptional regulator, winged helix family  60.99 
 
 		 225 aa  269  2.9999999999999997e-71  Burkholderia phytofirmans PsJN  Bacteria  normal  0.57611  normal  1 
 
 
-
 
NC_010515  Bcenmc03_4106  two component heavy metal response transcriptional regulator  58.11 
 
 		 223 aa  268  5e-71  Burkholderia cenocepacia MC0-3  Bacteria  normal  1  normal  0.971428 
 
 
-
 
NC_007952  Bxe_B1389  two component heavy metal response transcriptional regulator  60.54 
 
 		 225 aa  267  1e-70  Burkholderia xenovorans LB400  Bacteria  normal  0.717226  normal  0.091392 
 
 
-
 
NC_008061  Bcen_4757  two component heavy metal response transcriptional regulator  57.66 
 
 		 223 aa  266  2e-70  Burkholderia cenocepacia AU 1054  Bacteria  normal  0.0878196  n/a   
 
 
-
 
NC_008543  Bcen2424_3410  two component heavy metal response transcriptional regulator  57.66 
 
 		 223 aa  266  2e-70  Burkholderia cenocepacia HI2424  Bacteria  normal  1  normal  1 
 
 
-
 
NC_011662  Tmz1t_2101  two component heavy metal response transcriptional regulator, winged helix family  59.47 
 
 		 228 aa  266  2.9999999999999995e-70  Thauera sp. MZ1T  Bacteria  normal  1  n/a   
 
 
-
 
NC_011667  Tmz1t_2388  heavy metal response regulator  59.47 
 
 		 228 aa  266  2.9999999999999995e-70  Thauera sp. MZ1T  Bacteria  decreased coverage  0.000142984  normal  0.119734 
 
 
-
 
NC_010557  BamMC406_5744  two component heavy metal response transcriptional regulator  59.64 
 
 		 225 aa  265  4e-70  Burkholderia ambifaria MC40-6  Bacteria  normal  0.0360294  normal  0.749662 
 
 
-
 
NC_010623  Bphy_3962  two component heavy metal response transcriptional regulator  59.64 
 
 		 225 aa  265  5e-70  Burkholderia phymatum STM815  Bacteria  normal  1  normal  1 
 
 
-
 
NC_010512  Bcenmc03_6987  two component heavy metal response transcriptional regulator  59.56 
 
 		 225 aa  265  5.999999999999999e-70  Burkholderia cenocepacia MC0-3  Bacteria  normal  0.67799  normal  0.586494 
 
 
-
 
NC_008060  Bcen_1505  two component heavy metal response transcriptional regulator  59.56 
 
 		 225 aa  265  5.999999999999999e-70  Burkholderia cenocepacia AU 1054  Bacteria  normal  0.228186  n/a   
 
 
-
 
NC_008544  Bcen2424_6324  two component heavy metal response transcriptional regulator  59.56 
 
 		 225 aa  265  5.999999999999999e-70  Burkholderia cenocepacia HI2424  Bacteria  normal  0.313253  normal  1 
 
 
-
 
NC_011992  Dtpsy_2215  two component heavy metal response transcriptional regulator, winged helix family  60.18 
 
 		 228 aa  264  8e-70  Acidovorax ebreus TPSY  Bacteria  normal  1  n/a   
 
 
-
 
NC_008392  Bamb_5988  two component heavy metal response transcriptional regulator  59.64 
 
 		 225 aa  262  3e-69  Burkholderia ambifaria AMMD  Bacteria  normal  1  normal  1 
 
 
-
 
NC_007348  Reut_B3961  heavy metal response regulator  58.67 
 
 		 229 aa  261  4e-69  Ralstonia eutropha JMP134  Bacteria  decreased coverage  0.00889091  n/a   
 
 
-
 
NC_010682  Rpic_1760  two component heavy metal response transcriptional regulator, winged helix family  56.82 
 
 		 225 aa  261  8e-69  Ralstonia pickettii 12J  Bacteria  normal  0.133831  normal  0.402692 
 
 
-
 
NC_007511  Bcep18194_B2768  two component heavy metal response transcriptional regulator  58.11 
 
 		 223 aa  259  2e-68  Burkholderia sp. 383  Bacteria  normal  1  normal  1 
 
 
-
 
NC_007973  Rmet_1751  two component heavy metal response transcriptional regulator  57.66 
 
 		 224 aa  259  2e-68  Cupriavidus metallidurans CH34  Bacteria  normal  1  normal  0.0784096 
 
 
-
 
NC_008758  Pnap_4558  two component heavy metal response transcriptional regulator  56.95 
 
 		 226 aa  259  2e-68  Polaromonas naphthalenivorans CJ2  Bacteria  normal  1  normal  1 
 
 
-
 
NC_007971  Rmet_5978  regulatory protein CzcR  56.82 
 
 		 225 aa  258  4e-68  Cupriavidus metallidurans CH34  Bacteria  hitchhiker  0.0000269255  normal  0.0723212 
 
 
-
 
NC_010070  Bmul_6295  two component heavy metal response transcriptional regulator  56.31 
 
 		 223 aa  258  6e-68  Burkholderia multivorans ATCC 17616  Bacteria  normal  0.803537  normal  0.219108 
 
 
-
 
NC_011662  Tmz1t_3985  two component heavy metal response transcriptional regulator, winged helix family  58.56 
 
 		 226 aa  257  1e-67  Thauera sp. MZ1T  Bacteria  normal  1  n/a   
 
 
-
 
NC_007492  Pfl01_3379  two component heavy metal response transcriptional regulator  56.22 
 
 		 223 aa  256  2e-67  Pseudomonas fluorescens Pf0-1  Bacteria  normal  1  normal  1 
 
 
-
 
NC_009656  PSPA7_2348  putative two-component response regulator  57.08 
 
 		 228 aa  256  2e-67  Pseudomonas aeruginosa PA7  Bacteria  normal  0.804247  n/a   
 
 
-
 
NC_007952  Bxe_B1748a  hypothetical protein  57.08 
 
 		 223 aa  253  1.0000000000000001e-66  Burkholderia xenovorans LB400  Bacteria  normal  0.0697031  normal  0.305387 
 
 
-
 
NC_008463  PA14_27810  putative two-component response regulator  57.27 
 
 		 226 aa  253  2.0000000000000002e-66  Pseudomonas aeruginosa UCBPP-PA14  Bacteria  normal  1  normal  1 
 
 
-
 
NC_010676  Bphyt_4933  two component heavy metal response transcriptional regulator, winged helix family  56.62 
 
 		 223 aa  252  3e-66  Burkholderia phytofirmans PsJN  Bacteria  normal  0.461594  normal  0.344735 
 
 
-
 
NC_011662  Tmz1t_4055  two component heavy metal response transcriptional regulator, winged helix family  57.21 
 
 		 226 aa  251  7e-66  Thauera sp. MZ1T  Bacteria  normal  1  n/a   
 
 
-
 
NC_010322  PputGB1_1699  two component heavy metal response transcriptional regulator  57.59 
 
 		 226 aa  250  1e-65  Pseudomonas putida GB-1  Bacteria  normal  1  hitchhiker  0.00419772 
 
 
-
 
NC_009439  Pmen_1174  two component heavy metal response transcriptional regulator  55.16 
 
 		 224 aa  250  2e-65  Pseudomonas mendocina ymp  Bacteria  normal  0.216853  normal  1 
 
 
-
 
NC_007005  Psyr_1497  heavy metal response regulator  57.89 
 
 		 228 aa  249  2e-65  Pseudomonas syringae pv. syringae B728a  Bacteria  normal  1  normal  1 
 
 
-
 
NC_007298  Daro_2474  heavy metal response regulator  56.95 
 
 		 223 aa  249  4e-65  Dechloromonas aromatica RCB  Bacteria  normal  1  normal  1 
 
 
-
 
NC_002947  PP_2158  two component heavy metal response transcriptional regulator  56.7 
 
 		 226 aa  248  5e-65  Pseudomonas putida KT2440  Bacteria  normal  0.144347  normal  0.036827 
 
 
-
 
NC_009467  Acry_3106  two component heavy metal response transcriptional regulator  55.51 
 
 		 225 aa  248  7e-65  Acidiphilium cryptum JF-5  Bacteria  normal  1  n/a   
 
 
-
 
NC_009512  Pput_3584  two component heavy metal response transcriptional regulator  56.7 
 
 		 226 aa  247  1e-64  Pseudomonas putida F1  Bacteria  normal  1  normal  1 
 
 
-
 
NC_011313  VSAL_II0197  transcriptional activator protein, response regulator  52.44 
 
 		 227 aa  246  2e-64  Aliivibrio salmonicida LFI1238  Bacteria  hitchhiker  0.0086442  n/a   
 
 
-
 
NC_010002  Daci_1713  two component heavy metal response transcriptional regulator  54.63 
 
 		 227 aa  246  3e-64  Delftia acidovorans SPH-1  Bacteria  normal  1  normal  0.535279 
 
 
-
 
NC_009483  Gura_1986  two component heavy metal response transcriptional regulator  53.81 
 
 		 223 aa  244  6.999999999999999e-64  Geobacter uraniireducens Rf4  Bacteria  normal  0.0566795  n/a   
 
 
-
 
NC_012849  Rpic12D_5099  two component heavy metal response transcriptional regulator, winged helix family  55.95 
 
 		 228 aa  244  8e-64  Ralstonia pickettii 12D  Bacteria  normal  1  normal  0.0773075 
 
 
-
 
NC_011901  Tgr7_1208  two component heavy metal response transcriptional regulator, winged helix family  58.56 
 
 		 226 aa  244  8e-64  Thioalkalivibrio sp. HL-EbGR7  Bacteria  normal  1  n/a   
 
 
-
 
NC_010501  PputW619_0017  two component heavy metal response transcriptional regulator  56.31 
 
 		 225 aa  244  9.999999999999999e-64  Pseudomonas putida W619  Bacteria  normal  1  normal  1 
 
 
-
 
NC_009512  Pput_0017  two component heavy metal response transcriptional regulator  56.31 
 
 		 225 aa  244  9.999999999999999e-64  Pseudomonas putida F1  Bacteria  normal  1  normal  0.0468746 
 
 
-
 
NC_012880  Dd703_3722  two component heavy metal response transcriptional regulator, winged helix family  53.6 
 
 		 223 aa  243  9.999999999999999e-64  Dickeya dadantii Ech703  Bacteria  normal  1  n/a   
 
 
-
 
NC_010322  PputGB1_0019  two component heavy metal response transcriptional regulator  56.31 
 
 		 225 aa  244  9.999999999999999e-64  Pseudomonas putida GB-1  Bacteria  normal  0.593373  normal  1 
 
 
-
 
NC_010002  Daci_0451  two component heavy metal response transcriptional regulator  56.19 
 
 		 230 aa  244  9.999999999999999e-64  Delftia acidovorans SPH-1  Bacteria  normal  1  normal  1 
 
 
-
 
NC_007435  BURPS1710b_A1103  DNA-binding response regulator IrlR  53.07 
 
 		 230 aa  243  1.9999999999999999e-63  Burkholderia pseudomallei 1710b  Bacteria  normal  0.0630149  n/a   
 
 
-
 
NC_009075  BURPS668_A2869  transcriptional activator protein IrlR  53.07 
 
 		 230 aa  243  1.9999999999999999e-63  Burkholderia pseudomallei 668  Bacteria  normal  0.354021  n/a   
 
 
-
 
NC_007511  Bcep18194_B2430  two component heavy metal response transcriptional regulator  54.95 
 
 		 227 aa  243  1.9999999999999999e-63  Burkholderia sp. 383  Bacteria  normal  1  normal  1 
 
 
-
 
NC_010515  Bcenmc03_3842  two component heavy metal response transcriptional regulator  54.95 
 
 		 227 aa  243  1.9999999999999999e-63  Burkholderia cenocepacia MC0-3  Bacteria  normal  1  normal  0.67414 
 
 
-
 
NC_013456  VEA_001641  putative two component response regulator transcription regulator protein  52.61 
 
 		 230 aa  243  1.9999999999999999e-63  Vibrio sp. Ex25  Bacteria  normal  0.170142  n/a   
 
 
-
 
NC_010658  SbBS512_E0914  transcriptional regulatory protein YedW  53.39 
 
 		 260 aa  243  1.9999999999999999e-63  Shigella boydii CDC 3083-94  Bacteria  decreased coverage  0.0000000013212  n/a   
 
 
-
 
NC_007952  Bxe_B0708  two component heavy metal response transcriptional regulator  53.54 
 
 		 226 aa  243  1.9999999999999999e-63  Burkholderia xenovorans LB400  Bacteria  normal  0.328024  normal  0.9016 
 
 
-
 
NC_009078  BURPS1106A_A2716  DNA-binding heavy metal response regulator  53.07 
 
 		 230 aa  243  1.9999999999999999e-63  Burkholderia pseudomallei 1106a  Bacteria  normal  0.558922  n/a   
 
 
-
 
NC_010498  EcSMS35_1216  transcriptional regulatory protein YedW  53.39 
 
 		 260 aa  243  1.9999999999999999e-63  Escherichia coli SMS-3-5  Bacteria  decreased coverage  0.000000203871  normal  0.637592 
 
 
-
 
NC_008061  Bcen_4683  two component heavy metal response transcriptional regulator  54.95 
 
 		 228 aa  243  1.9999999999999999e-63  Burkholderia cenocepacia AU 1054  Bacteria  normal  0.222547  n/a   
 
 
-
 
NC_008543  Bcen2424_3680  two component heavy metal response transcriptional regulator  54.95 
 
 		 228 aa  243  1.9999999999999999e-63  Burkholderia cenocepacia HI2424  Bacteria  normal  0.224842  normal  1 
 
 
-
 
CP001637  EcDH1_1682  two component heavy metal response transcriptional regulator, winged helix family  53.39 
 
 		 260 aa  242  3e-63  Escherichia coli DH1  Bacteria  decreased coverage  0.000000000000361446  n/a   
 
 
-
 
NC_009801  EcE24377A_2200  transcriptional regulatory protein YedW  53.39 
 
 		 260 aa  243  3e-63  Escherichia coli E24377A  Bacteria  decreased coverage  0.0000000000454941  n/a   
 
 
-
 
NC_009800  EcHS_A2070  transcriptional regulatory protein YedW  53.39 
 
 		 260 aa  243  3e-63  Escherichia coli HS  Bacteria  hitchhiker  3.15395e-18  n/a   
 
 
-
 
NC_010468  EcolC_1676  transcriptional regulatory protein YedW  53.39 
 
 		 260 aa  243  3e-63  Escherichia coli ATCC 8739  Bacteria  decreased coverage  0.000000350308  normal  0.20506 
 
 
-
 
NC_010682  Rpic_1718  two component heavy metal response transcriptional regulator, winged helix family  55.26 
 
 		 228 aa  242  3e-63  Ralstonia pickettii 12J  Bacteria  normal  0.348336  normal  1 
 
 
-
 
NC_010676  Bphyt_4389  two component heavy metal response transcriptional regulator, winged helix family  53.54 
 
 		 226 aa  242  3e-63  Burkholderia phytofirmans PsJN  Bacteria  normal  1  normal  1 
 
 
-
 
NC_012849  Rpic12D_5335  two component heavy metal response transcriptional regulator, winged helix family  55.26 
 
 		 228 aa  242  3e-63  Ralstonia pickettii 12D  Bacteria  normal  0.733276  normal  0.970872 
 
 
-
 
CP001509  ECD_01884  predicted DNA-binding response regulator in two-component system with YedV  53.39 
 
 		 223 aa  242  3.9999999999999997e-63  Escherichia coli BL21(DE3)  Bacteria  unclonable  0.000000533293  n/a   
 
 
-
 
NC_002947  PP_5383  two component heavy metal response transcriptional regulator  56.5 
 
 		 225 aa  242  3.9999999999999997e-63  Pseudomonas putida KT2440  Bacteria  normal  1  normal  0.651671 
 
 
-
 
NC_007650  BTH_II1376  DNA-binding response regulator IrlR  54.5 
 
 		 229 aa  242  3.9999999999999997e-63  Burkholderia thailandensis E264  Bacteria  normal  0.498923  n/a   
 
 
-
 
NC_012892  B21_01874  hypothetical protein  53.39 
 
 		 223 aa  242  3.9999999999999997e-63  Escherichia coli BL21  Bacteria  unclonable  0.00000061937  n/a   
 
 
-
 
NC_007005  Psyr_3374  heavy metal response regulator  54.5 
 
 		 229 aa  241  5e-63  Pseudomonas syringae pv. syringae B728a  Bacteria  normal  1  normal  1 
 
 
-
 
NC_011353  ECH74115_2748  transcriptional regulatory protein YedW  53.39 
 
 		 233 aa  241  5e-63  Escherichia coli O157:H7 str. EC4115  Bacteria  hitchhiker  0.00000892088  normal  0.227347 
 
 
-
 
NC_010682  Rpic_1841  two component heavy metal response transcriptional regulator, winged helix family  55.07 
 
 		 228 aa  241  5e-63  Ralstonia pickettii 12J  Bacteria  normal  0.99331  hitchhiker  0.00000222676 
 
 
-
 
NC_010070  Bmul_6173  two component heavy metal response transcriptional regulator  54.95 
 
 		 228 aa  241  5e-63  Burkholderia multivorans ATCC 17616  Bacteria  normal  1  normal  0.400074 
 
 
-
 
NC_010084  Bmul_2337  two component heavy metal response transcriptional regulator  56 
 
 		 227 aa  241  5e-63  Burkholderia multivorans ATCC 17616  Bacteria  normal  1  normal  1 
 
 
-
 
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